转录组分析揭示了胆管癌的氧化应激相关特征和分子亚型。

IF 2.3 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Zichao Wu
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引用次数: 0

摘要

胆管癌(CCA)是一种异质性侵袭性恶性肿瘤,治疗方案有限,预后较差。鉴定可靠的预后生物标志物和加深对分子亚型的了解对于开发靶向疗法和改善患者预后至关重要。本研究旨在发现CCA中的氧化应激相关基因(ORGs),并利用癌症基因组图谱(TCGA)的全面转录组分析建立预后风险模型。通过LASSO回归分析,我们确定了与预后相关的ORGs,并构建了由6个ORGs组成的预后特征。该特征在生存分析和 ROC 曲线评估中表现出很强的预测能力。功能富集和GSEA分析显示,免疫相关通路在不同风险组别中显著富集。GSVA分析表明,高风险亚组的炎症和氧化应激通路活性降低,xCell结果显示该组的免疫细胞浸润水平较低。此外,免疫检查点基因和免疫相关通路在高风险亚组中下调。我们的研究建立了一个独特的预后模型,重点关注氧化应激,能够准确预测患者的预后,并为 CCA 患者的预后提供重要的见解和建议。未来的研究应着眼于在临床环境中验证这些发现,并进一步探索氧化应激通路中的治疗靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Transcriptomic analysis reveals oxidative stress-related signature and molecular subtypes in cholangio carcinoma.

Transcriptomic analysis reveals oxidative stress-related signature and molecular subtypes in cholangio carcinoma.

Cholangiocarcinoma (CCA) is a heterogeneous and aggressive malignancy with limited therapeutic options and poor prognosis. The identification of reliable prognostic biomarkers and a deeper understanding of the molecular subtypes are critical for the development of targeted therapies and improvement of patient outcomes. This study aims to uncover oxidative stress-related genes (ORGs) in CCA and develop a prognostic risk model using comprehensive transcriptomic analysis from The Cancer Genome Atlas (TCGA). Through LASSO regression analysis, we identified prognosis-related ORGs and constructed a prognostic signature consisting of six ORGs. This signature demonstrated strong predictive performance in survival analysis and ROC curve assessment. Functional enrichment and GSEA analyses revealed significant enrichment of immune-related pathways among different risk groups. GSVA analysis indicated reduced activity in inflammation and oxidative stress pathways in the high-risk subgroup, and xCell results showed lower immune cell infiltration levels in this group. Additionally, immune checkpoint genes and immune-related pathways were downregulated in the high-risk subgroup. Our research has developed a unique prognostic model focusing on oxidative stress, enabling accurate forecasting of patient outcomes and providing crucial insights and recommendations for the prognosis of individuals with CCA. Future studies should aim to validate these findings in clinical settings and further explore therapeutic targets within oxidative stress pathways.

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来源期刊
Molecular Genetics and Genomics
Molecular Genetics and Genomics 生物-生化与分子生物学
CiteScore
5.10
自引率
3.20%
发文量
134
审稿时长
1 months
期刊介绍: Molecular Genetics and Genomics (MGG) publishes peer-reviewed articles covering all areas of genetics and genomics. Any approach to the study of genes and genomes is considered, be it experimental, theoretical or synthetic. MGG publishes research on all organisms that is of broad interest to those working in the fields of genetics, genomics, biology, medicine and biotechnology. The journal investigates a broad range of topics, including these from recent issues: mechanisms for extending longevity in a variety of organisms; screening of yeast metal homeostasis genes involved in mitochondrial functions; molecular mapping of cultivar-specific avirulence genes in the rice blast fungus and more.
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