利用 CRISPR-Combo 系统对番茄进行正交基因组编辑和转录激活。

IF 5.3 2区生物学 Q1 PLANT SCIENCES

Plant Cell Reports Pub Date : 2024-09-05 DOI:10.1007/s00299-024-03316-6

Innocent Byiringiro, Changtian Pan, Yiping Qi

引用次数: 0

摘要

关键信息CRISPR-Combo系统（Cas9-Combo和CBE-Combo）设计用于综合遗传操作，可在番茄稳定品系中实现基于Cas9的靶向诱变或胞嘧啶碱基编辑，并同时激活基因。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Orthogonal genome editing and transcriptional activation in tomato using CRISPR-Combo systems.

查看原文本刊更多论文

Orthogonal genome editing and transcriptional activation in tomato using CRISPR-Combo systems.

Key message: The CRISPR-Combo systems (Cas9-Combo and CBE-Combo) are designed for comprehensive genetic manipulation, enabling Cas9-based targeted mutagenesis or cytosine base editing with simultaneous gene activation in tomato stable lines.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Plant Cell Reports 生物-植物科学

CiteScore

10.80

自引率

1.60%

发文量

135

审稿时长

3.2 months

期刊介绍： Plant Cell Reports publishes original, peer-reviewed articles on new advances in all aspects of plant cell science, plant genetics and molecular biology. Papers selected for publication contribute significant new advances to clearly identified technological problems and/or biological questions. The articles will prove relevant beyond the narrow topic of interest to a readership with broad scientific background. The coverage includes such topics as: - genomics and genetics - metabolism - cell biology - abiotic and biotic stress - phytopathology - gene transfer and expression - molecular pharming - systems biology - nanobiotechnology - genome editing - phenomics and synthetic biology The journal also publishes opinion papers, review and focus articles on the latest developments and new advances in research and technology in plant molecular biology and biotechnology.