Kyle Keepers, Kelsey Peterson, Andrew Raduski, Kathryn M. Turner, David Van Tassel, Kevin Smith, Alex Harkess, James D. Bever, Yaniv Brandvain
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引用次数: 0
摘要
植物对病原体的细胞内防御是由一类抗病基因(NB-LRRs 或 NLRs,R 基因)介导的。这些基因所抵御的许多病害在降雨量较高的地区更为普遍,这为病原体提供了更好的生长条件。因此,我们预计在适应较潮湿条件的植物中,R 基因的维持和增殖将面临更大的选择压力。在本研究中,我们使用 RenSeq 从主要由普通向日葵(Helianthus annuus)参考基因组开发的饵料中富集了 R 基因文库。我们采用 Illumina 短读数(n = 99)和 PacBio 长读数(n = 10)方法,对美国中部平原 12 个草原残留区的向日葵多年生亲缘植物 Silphium integrifolium Michx 的 R 基因文库进行了测序。我们发现,植物来源地草原残留物的年平均有效降水量与其基因组中的 R 基因数量之间存在正相关关系,这与植物病原体共同进化的强度随降水量增加而增加是一致的。我们发现 RenSeq 可用于研究模式生物非模式近缘种的生态假说。
Disease resistance gene count increases with rainfall in Silphium integrifolium
Intracellular plant defense against pathogens is mediated by a class of disease resistance genes known as NB-LRRs or NLRs (R genes). Many of the diseases these genes protect against are more prevalent in regions of higher rainfall, which provide better growth conditions for the pathogens. As such, we expect a higher selective pressure for the maintenance and proliferation of R genes in plants adapted to wetter conditions. In this study, we enriched libraries for R genes using RenSeq from baits primarily developed from the common sunflower (Helianthus annuus) reference genome. We sequenced the R gene libraries of Silphium integrifolium Michx, a perennial relative of sunflower, from 12 prairie remnants across a rainfall gradient in the Central Plains of the United States, with both Illumina short-read (n = 99) and PacBio long-read (n = 10) approaches. We found a positive relationship between the mean effective annual precipitation of a plant's source prairie remnant and the number of R genes in its genome, consistent with intensity of plant pathogen coevolution increasing with precipitation. We show that RenSeq can be applied to the study of ecological hypotheses in non-model relatives of model organisms.
期刊介绍:
Ecology and Evolution is the peer reviewed journal for rapid dissemination of research in all areas of ecology, evolution and conservation science. The journal gives priority to quality research reports, theoretical or empirical, that develop our understanding of organisms and their diversity, interactions between them, and the natural environment.
Ecology and Evolution gives prompt and equal consideration to papers reporting theoretical, experimental, applied and descriptive work in terrestrial and aquatic environments. The journal will consider submissions across taxa in areas including but not limited to micro and macro ecological and evolutionary processes, characteristics of and interactions between individuals, populations, communities and the environment, physiological responses to environmental change, population genetics and phylogenetics, relatedness and kin selection, life histories, systematics and taxonomy, conservation genetics, extinction, speciation, adaption, behaviour, biodiversity, species abundance, macroecology, population and ecosystem dynamics, and conservation policy.