低温电子显微镜：了解 RNA 分子动态世界的窗口

IF 6.1 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Current opinion in structural biology Pub Date : 2024-09-03 DOI:10.1016/j.sbi.2024.102916

Xiaojing Zhang, Shanshan Li, Kaiming Zhang

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引用次数: 0

摘要

RNA 对于复杂的细胞功能至关重要，其特点是结构多变，能够根据细胞线索发生构象转变。由于结构生物学技术的进步，包括核磁共振（NMR）、X 射线晶体学、冷冻电镜（cryo-EM）等，难以捉摸的 RNA 结构正以前所未有的清晰度被揭开。这篇综述重点探讨了冷冻电镜技术对我们理解 RNA 结构动力学的革命性影响，强调了该技术对结构生物学的贡献，并展望了这一快速发展领域的未来轨迹。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Cryo-EM: A window into the dynamic world of RNA molecules

RNAs are critical for complex cellular functions, characterized by their structural versatility and ability to undergo conformational transitions in response to cellular cues. The elusive structures of RNAs are being unraveled with unprecedented clarity, thanks to the technological advancements in structural biology, including nuclear magnetic resonance (NMR), X-ray crystallography, cryo-electron microscopy (cryo-EM) etc. This review focuses on examining the revolutionary impact of cryo-EM on our comprehension of RNA structural dynamics, underscoring the technique's contributions to structural biology and envisioning the future trajectory of this rapidly evolving field.

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来源期刊

Current opinion in structural biology 生物-生化与分子生物学

CiteScore

12.20

自引率

2.90%

发文量

179

审稿时长

6-12 weeks

期刊介绍： Current Opinion in Structural Biology (COSB) aims to stimulate scientifically grounded, interdisciplinary, multi-scale debate and exchange of ideas. It contains polished, concise and timely reviews and opinions, with particular emphasis on those articles published in the past two years. In addition to describing recent trends, the authors are encouraged to give their subjective opinion of the topics discussed. In COSB, we help the reader by providing in a systematic manner: 1. The views of experts on current advances in their field in a clear and readable form. 2. Evaluations of the most interesting papers, annotated by experts, from the great wealth of original publications. [...] The subject of Structural Biology is divided into twelve themed sections, each of which is reviewed once a year. Each issue contains two sections, and the amount of space devoted to each section is related to its importance. -Folding and Binding- Nucleic acids and their protein complexes- Macromolecular Machines- Theory and Simulation- Sequences and Topology- New constructs and expression of proteins- Membranes- Engineering and Design- Carbohydrate-protein interactions and glycosylation- Biophysical and molecular biological methods- Multi-protein assemblies in signalling- Catalysis and Regulation