建立测定大鼠全血中雷帕霉素浓度的 LC-MS/MS 方法

IF 1.6 4区 医学 Q4 BIOCHEMICAL RESEARCH METHODS
Yuanxia Yang, Miaoting Yang, Lijuan Wang, Liufang He, Tao Shi
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引用次数: 0

摘要

背景:建立和验证检测生物样本的方法是药代动力学研究的关键步骤。目前,有关雷帕霉素血浆浓度检测的方法学报告已发表数篇:本研究旨在探索一种检测大鼠全血生物样本中雷帕霉素的有效方法:本研究设计了一种快速、灵敏、特异的液相色谱-质谱/质谱联用仪(LC-MS/MS)检测大鼠全血生物样本中雷帕霉素的方法。我们全面验证了该方法的特异性、线性范围、定量下限(LLOQ)、精密度、准确度、回收率和稳定性:研究结果表明,LC-MS/MS 法检测雷帕霉素的灵敏度、特异性和定量分析的可靠性得到了证实。该方法通过我们经过验证的 LC-MS/MS 方法确保了后续研究数据的准确性和可靠性:研究结果表明,LC-MS/MS 方法可对大鼠全血样本中的雷帕霉素进行灵敏、特异和可靠的定量分析。该方法确保了后续研究数据的准确性和可靠性:本研究的重要意义在于成功建立了一种快速、灵敏、特异的 LC-MS/MS 方法,用于检测大鼠全血中雷帕霉素的浓度,确保了后续研究数据的准确性和可靠性。这为进一步了解雷帕霉素的体内代谢和药理作用提供了重要的工具和基础,有助于推进相关领域的药物研究和临床应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Establishment of LC-MS/MS Methodology for the Determination of Rapamycin Concentration in Rat Whole Blood.

Background: The establishment and validation of methods for testing biological samples are crucial steps in pharmacokinetic studies. Currently, several methodological reports have been published on the detection of rapamycin plasma concentrations.

Objective: The objective of this study was to explore an effective method for detecting rapamycin in rat whole blood biological samples.

Method: In this study, we designed a rapid, sensitive, and specific liquid chromatograph-mass spectrometer/mass spectrometer (LC-MS/MS) methodology for detecting rapamycin in rat whole blood biological samples. We comprehensively validated the specificity, linear range, lower limit of quantification (LLOQ), precision, accuracy, recovery, and stability of this method.

Results: The findings of this study confirmed the successful implementation of LC-MS/MS for the detection of rapamycin, demonstrating its sensitivity, specificity, and reliability in quantitative analysis. This method ensures the accuracy and reliability of subsequent study data through our validated LC-MS/MS approach.

Conclusion: The results demonstrated the successful implementation of an LC-MS/MS method for sensitive, specific, and reliable quantitative analysis of rapamycin in rat whole blood samples. This method ensures the accuracy and reliability of subsequent study data.

Significance: The importance of this study lies in the successful establishment of a rapid, sensitive, and specific LC-MS/MS method for detecting rapamycin concentration in rat whole blood, ensuring the accuracy and reliability of subsequent research data. This provides a crucial tool and foundation for further understanding the metabolism and pharmacological effects of rapamycin in vivo, aiding in the advancement of drug research and clinical applications in related fields.

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来源期刊
CiteScore
3.10
自引率
5.60%
发文量
327
审稿时长
7.5 months
期刊介绍: Combinatorial Chemistry & High Throughput Screening (CCHTS) publishes full length original research articles and reviews/mini-reviews dealing with various topics related to chemical biology (High Throughput Screening, Combinatorial Chemistry, Chemoinformatics, Laboratory Automation and Compound management) in advancing drug discovery research. Original research articles and reviews in the following areas are of special interest to the readers of this journal: Target identification and validation Assay design, development, miniaturization and comparison High throughput/high content/in silico screening and associated technologies Label-free detection technologies and applications Stem cell technologies Biomarkers ADMET/PK/PD methodologies and screening Probe discovery and development, hit to lead optimization Combinatorial chemistry (e.g. small molecules, peptide, nucleic acid or phage display libraries) Chemical library design and chemical diversity Chemo/bio-informatics, data mining Compound management Pharmacognosy Natural Products Research (Chemistry, Biology and Pharmacology of Natural Products) Natural Product Analytical Studies Bipharmaceutical studies of Natural products Drug repurposing Data management and statistical analysis Laboratory automation, robotics, microfluidics, signal detection technologies Current & Future Institutional Research Profile Technology transfer, legal and licensing issues Patents.
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