给育肥兔补充桑叶 1-脱氧野尻霉素可缩短绒毛高度并改善肠道屏障

IF 2.4 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Animal Bioscience Pub Date : 2024-12-01 Epub Date: 2024-08-22 DOI:10.5713/ab.24.0109
Shaocong Li, Tao Li, Zijie Jiang, Wenyu Hou, Qirui Hou, Boris Ramos Serrano, Adileidys Ruiz Barcenas, Yuhua Wang, Weiguo Zhao
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引用次数: 0

摘要

目的本研究探讨桑椹1-脱氧野尻霉素(DNJ)对家兔消化能力、肠道形态和肠道屏障的影响:饲养36只45日龄左右的新西兰白兔(雄性)(平均体重为1.05 0.04 kg),采用商品日粮,然后将其分为三组(n= 12),在饲料中添加不同水平的DNJ提取物:结果表明:T0(0 克/千克)、T1(0.35 克/千克)、T2(0.7 克/千克)饲养 28 d:结果表明,T2 降低了平均日增重(ADG)(P < 0.05)。与 T0 相比,T1 和 T2 降低了绒毛高度和炎症因子水平(P < 0.05)。DNJ 明显降低了戊酸的含量(P < 0.05)。T1 中乙酸、丙酸、异丁酸和异戊酸的含量高于 T0 和 T2(P < 0.05)。T2 中丁酸的含量低于 T0 和 T1(P < 0.05)。随着 DNJ 浓度的提高,己酸的含量先提高后降低(P < 0.05)。与 T0 相比,T2 明显增加了 dgA-11_gut_group 和 Christensenellaceae_R-7_group 的数量,而减少了 Bacteroide 和 Ralstonia 的数量(P < 0.05)。与 T0 相比,T1 和 T2 显著提高了 JAM2、JAM3、粘蛋白 4、粘蛋白 6 的基因表达量(P < 0.05),T1 显著降低了闭塞蛋白的表达量,而 T2 则显著提高了闭塞蛋白的表达量(P < 0.05),T2 显著提高了 claudin1 和 claudin2 的表达量(P < 0.05):结论:高水平的DNJ通过调节短链脂肪酸(SCFA)组成,改变了家兔空肠微生物组的组成,抑制了炎症,改善了肠道屏障,同时降低了家兔空肠的生长性能,缩短了绒毛高度。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Dietary Mulberry leaf 1-deoxynijirimycin supplementation shortens villus height and improves intestinal barrier in fattening rabbits.

Objective: The current study investigated the effects of mulberry 1-deoxynijirimycin (DNJ) on the digestion ability, intestinal morphology, and intestinal barrier of rabbits.

Methods: A total of 36 New Zealand White rabbits (male) about 45 days old (mean body weight of 1.05±0.04 kg) were reared and commercial diets were employed, and afterwards divided into three groups (n = 12) with different levels of DNJ extract additive in feed: T0 (0 g/kg), T1 (0.35 g/kg), T2 (0.7 g/kg) for 28 d.

Results: The results demonstrated that T2 decreased the average daily gain (p<0.05). T1 and T2 decreased villus height and inflammatory factor levels as compared with T0 (p<0.05). DNJ significantly decreased the content of valeric acid (p<0.05). The content of acetic acid, propionic acid, iso butyric acid, iso valeric acid in T1 were higher than those in T0 and T2 (p<0.05). The content of butyric acid in T2 was lower than it in T0 and T1 (p<0.05). The content of caproic acid was firstly improved then reduced as the DNJ concentration improved (p<0.05). T2 significantly increased the abundance of dgA-11_gut_group and Christensenellaceae_R-7_group while decreased Bacteroide and Ralstonia as compared with T0 (p<0.05). Compared with T0, T1, and T2 significantly improved the gene expression of JAM2, JAM3, mucin4, mucin6 (p<0.05), T1 significantly decreased the expression of occluding while T2 significantly increased (p<0.05), T2 significantly increased the expression of claudin1 and claudin2 (p<0.05).

Conclusion: DNJ at high level changed microbiome compositions, inhibited inflammation, and improved intestinal barrier while it decreased the growth performance and shorted villus height in rabbit jejunum by regulating short chain fatty acid compositions in rabbits.

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来源期刊
Animal Bioscience
Animal Bioscience AGRICULTURE, DAIRY & ANIMAL SCIENCE-
CiteScore
5.00
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223
审稿时长
3 months
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