利用酵母工程技术从单一甲醇中高水平生产 β-法呢烯。

IF 6.8 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
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引用次数: 0

摘要

甲醇是一种丰富的一碳原料,可通过液态阳光路线从二氧化碳中大量生产甲醇,有助于实现碳中和。β-法呢烯广泛应用于聚合物、表面活性剂、润滑剂的生产,也可作为航空燃料的合适替代品。构建高效的细胞工厂是通过甲醇生物转化生产 β-法呢烯的可行方法。在此,我们广泛改造了养甲酵母 Ogataea polymorpha,以甲醇为唯一碳源,高效生物生产 β-法呢烯。我们的研究表明,在优良的酵母底盘中提供充足的前体乙酰-CoA 和辅助因子 NADPH,β-法呢烯的产量比野生型背景菌株高出 1.3 倍。通过进一步优化甲羟戊酸途径和增加乙酰-CoA的供应,β-法呢烯的积累量增加了7倍,在生物反应器中进行饲料批量发酵时,单碳原料的倍半萜类化合物产量(14.7克/升,甲醇产量为46毫克/克)达到了目前报道的最高水平。这项研究表明,O. polymorpha 在利用甲醇生产高级萜类化合物方面具有巨大的工程潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Engineering yeast for high-level production of β-farnesene from sole methanol

Methanol, a rich one-carbon feedstock, can be massively produced from CO2 by the liquid sunshine route, which is helpful to realize carbon neutrality. β-Farnesene is widely used in the production of polymers, surfactants, lubricants, and also serves as a suitable substitute for jet fuel. Constructing an efficient cell factory is a feasible approach for β-farnesene production through methanol biotransformation. Here, we extensively engineered the methylotrophic yeast Ogataea polymorpha for the efficient bio-production of β-farnesene using methanol as the sole carbon source. Our study demonstrated that sufficient supply of precursor acetyl-CoA and cofactor NADPH in an excellent yeast chassis had a 1.3-fold higher β-farnesene production than that of wild-type background strain. Further optimization of the mevalonate pathway and enhancement of acetyl-CoA supply led to a 7-fold increase in β-farnesene accumulation, achieving the highest reported sesquiterpenoids production (14.7 g/L with a yield of 46 mg/g methanol) from one-carbon feedstock under fed-batch fermentation in bioreactor. This study demonstrates the great potential of engineering O. polymorpha for high-level terpenoid production from methanol.

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来源期刊
Metabolic engineering
Metabolic engineering 工程技术-生物工程与应用微生物
CiteScore
15.60
自引率
6.00%
发文量
140
审稿时长
44 days
期刊介绍: Metabolic Engineering (MBE) is a journal that focuses on publishing original research papers on the directed modulation of metabolic pathways for metabolite overproduction or the enhancement of cellular properties. It welcomes papers that describe the engineering of native pathways and the synthesis of heterologous pathways to convert microorganisms into microbial cell factories. The journal covers experimental, computational, and modeling approaches for understanding metabolic pathways and manipulating them through genetic, media, or environmental means. Effective exploration of metabolic pathways necessitates the use of molecular biology and biochemistry methods, as well as engineering techniques for modeling and data analysis. MBE serves as a platform for interdisciplinary research in fields such as biochemistry, molecular biology, applied microbiology, cellular physiology, cellular nutrition in health and disease, and biochemical engineering. The journal publishes various types of papers, including original research papers and review papers. It is indexed and abstracted in databases such as Scopus, Embase, EMBiology, Current Contents - Life Sciences and Clinical Medicine, Science Citation Index, PubMed/Medline, CAS and Biotechnology Citation Index.
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