A Saedi, S Zarei, M Vatanparast, M R Hajizadeh, R Hosseiniara, O S Esmaeili, M Mohammad-Sadeghipour, Z Mirzaei, M Mahmoodi
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SAE and Met were orally administered at doses of 400mg/kg/d and 250mg/kg/d on day 22 and continued for an additional 28 days. Vaginal smears were analyzed, and gene expression levels of GLUT4, SIRT1, TNF-α, and INSR were evaluated using RT-qPCR. Antioxidant parameters were assessed using detection kits.</p><p><strong>Results: </strong>Treatment with SAE and Met restored a regular estrous cycle pattern in PCOS rats. Furthermore, SAE and Met treatment improved hormonal balance, dyslipidemia, and hyperglycemia in the rats. Administration of SAE and Met significantly elevated levels of antioxidant enzymes SOD and GPx in ovarian tissue (P<0.001). Additionally, mRNA levels of GLUT4, SIRT1, and INSR were significantly increased in ovarian tissue following SAE and Met treatment, while TNF-α gene expression decreased significantly (P<0.0001).</p><p><strong>Conclusion: </strong>The findings suggest that SAE and Met aqueous extract exert protective effects on letrozole-induced PCOS in rats by modulating gene expression associated with insulin signaling and oxidative stress.</p>","PeriodicalId":8332,"journal":{"name":"Annales pharmaceutiques francaises","volume":null,"pages":null},"PeriodicalIF":1.0000,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Therapeutic effects of stevia aqueous extract alone or in combination with metformin in induced polycystic ovary syndrome rats: Gene expression, hormonal balance, and metabolomics aspects.\",\"authors\":\"A Saedi, S Zarei, M Vatanparast, M R Hajizadeh, R Hosseiniara, O S Esmaeili, M Mohammad-Sadeghipour, Z Mirzaei, M Mahmoodi\",\"doi\":\"10.1016/j.pharma.2024.08.008\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objectives: </strong>This study aimed to assess the individual and combined effects of SAE and Met on the expression of genes related to insulin signaling, oxidative stress, hormonal imbalance, insulin resistance, and dyslipidemia in rats with induced PCOS.</p><p><strong>Methods: </strong>The estrous cycle of 50 adult Wistar female rats was monitored through vaginal smears. 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引用次数: 0
摘要
研究目的本研究旨在评估SAE和Met对诱导多囊卵巢综合征大鼠胰岛素信号转导、氧化应激、内分泌失调、胰岛素抵抗和血脂异常相关基因表达的单独和联合影响:方法:通过阴道涂片监测50只成年Wistar雌性大鼠的发情周期。随后,大鼠被随机分为5组,每组10只,包括对照组(接受1毫升羧甲基纤维素,共49天)、诱导组(来曲唑,1毫克/千克/天,共21天)、SAE组、Met组和SAE/Met组。第22天开始口服SAE和Met,剂量分别为400毫克/千克/天和250毫克/千克/天,并持续28天。对阴道涂片进行分析,并使用 RT-qPCR 评估 GLUT4、SIRT1、TNF-α 和 INSR 的基因表达水平。使用检测试剂盒评估了抗氧化参数:结果:用SAE和Met治疗后,多囊卵巢综合征大鼠恢复了正常的发情周期模式。此外,SAE 和 Met 治疗还改善了大鼠的激素平衡、血脂异常和高血糖。服用 SAE 和 Met 能明显提高卵巢组织中抗氧化酶 SOD 和 GPx 的水平(PC 结论:研究结果表明,SAE 和 Met 能提高卵巢组织中抗氧化酶 SOD 和 GPx 的水平:研究结果表明,SAE和Met水提取物通过调节与胰岛素信号传导和氧化应激相关的基因表达,对来曲唑诱导的多囊卵巢综合征大鼠具有保护作用。
Therapeutic effects of stevia aqueous extract alone or in combination with metformin in induced polycystic ovary syndrome rats: Gene expression, hormonal balance, and metabolomics aspects.
Objectives: This study aimed to assess the individual and combined effects of SAE and Met on the expression of genes related to insulin signaling, oxidative stress, hormonal imbalance, insulin resistance, and dyslipidemia in rats with induced PCOS.
Methods: The estrous cycle of 50 adult Wistar female rats was monitored through vaginal smears. Subsequently, the rats were randomly assigned into five groups of 10, including control (receiving 1ml of carboxymethyl cellulose for 49 days), induction (letrozole at 1mg/kg/d for 21 days), SAE, Met, and SAE/Met. SAE and Met were orally administered at doses of 400mg/kg/d and 250mg/kg/d on day 22 and continued for an additional 28 days. Vaginal smears were analyzed, and gene expression levels of GLUT4, SIRT1, TNF-α, and INSR were evaluated using RT-qPCR. Antioxidant parameters were assessed using detection kits.
Results: Treatment with SAE and Met restored a regular estrous cycle pattern in PCOS rats. Furthermore, SAE and Met treatment improved hormonal balance, dyslipidemia, and hyperglycemia in the rats. Administration of SAE and Met significantly elevated levels of antioxidant enzymes SOD and GPx in ovarian tissue (P<0.001). Additionally, mRNA levels of GLUT4, SIRT1, and INSR were significantly increased in ovarian tissue following SAE and Met treatment, while TNF-α gene expression decreased significantly (P<0.0001).
Conclusion: The findings suggest that SAE and Met aqueous extract exert protective effects on letrozole-induced PCOS in rats by modulating gene expression associated with insulin signaling and oxidative stress.
期刊介绍:
This journal proposes a scientific information validated and indexed to be informed about the last research works in all the domains interesting the pharmacy. The original works, general reviews, the focusing, the brief notes, subjected by the best academics and the professionals, propose a synthetic approach of the last progress accomplished in the concerned sectors. The thematic Sessions and the – life of the Academy – resume the communications which, presented in front of the national Academy of pharmacy, are in the heart of the current events.