PpEIL2/3-PpNAC1-PpWRKY14 模块通过调节乙烯的产生来调控桃的果实成熟。

IF 9.3 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Yudi Liu, Wen Xiao, Liao Liao, Beibei Zheng, Yunpeng Cao, Yun Zhao, Ruo-Xi Zhang, Yuepeng Han
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引用次数: 0

摘要

WRKY 转录因子在植物抵抗各种胁迫的过程中发挥着关键作用,但它们在果实成熟过程中的作用在很大程度上仍不为人所知。在此,我们报告了参与调控桃果实成熟的 WRKY 基因 PpWRKY14。在整个果实发育过程中,PpWRKY14 的表达呈上升趋势。PpWRKY14 是桃果实成熟主调控因子 PpNAC1 的靶基因。PpWRKY14 可直接与 PpACS1 和 PpACO1 的启动子结合,诱导它们的表达,当 PpWRKY14 与 PpNAC1 形成二聚体时,这种诱导作用大大增强。然而,PpNAC1 的转录可被两个 EIN3/EIL1 基因 PpEIL2 和 PpEIL3 直接抑制。PpEIL2/3 基因在果实发育早期高表达,但在成熟期其表达量会显著下降,从而抑制 PpNAC1 的表达。这些结果表明,PpEIL2/3-PpNAC1-PpWRKY14 模块通过调节乙烯的产生来调控桃果实的成熟。我们的研究结果有助于深入了解EIN3/EIL1和WRKY基因在果实成熟过程中的调控作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A PpEIL2/3-PpNAC1-PpWRKY14 module regulates fruit ripening by modulating ethylene production in peach.

WRKY transcription factors play key roles in plant resistance to various stresses, but their roles in fruit ripening remain largely unknown. Here, we report a WRKY gene PpWRKY14 involved in the regulation of fruit ripening in peach. The expression of PpWRKY14 showed an increasing trend throughout fruit development. PpWRKY14 was a target gene of PpNAC1, a master regulator of peach fruit ripening. PpWRKY14 could directly bind to the promoters of PpACS1 and PpACO1 to induce their expression, and this induction was greatly enhanced when PpWRKY14 formed a dimer with PpNAC1. However, the transcription of PpNAC1 could be directly suppressed by two EIN3/EIL1 genes, PpEIL2 and PpEIL3. The PpEIL2/3 genes were highly expressed at the early stages of fruit development, but their expression was programmed to decrease significantly during the ripening stage, thus derepressing the expression of PpNAC1. These results suggested a PpEIL2/3-PpNAC1-PpWRKY14 module that regulates fruit ripening by modulating ethylene production in peach. Our results provided an insight into the regulatory roles of EIN3/EIL1 and WRKY genes in fruit ripening.

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来源期刊
Journal of Integrative Plant Biology
Journal of Integrative Plant Biology 生物-生化与分子生物学
CiteScore
18.00
自引率
5.30%
发文量
220
审稿时长
3 months
期刊介绍: Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.
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