{"title":"用于治疗包皮手术后水肿的自组装生物活性寡肽水凝胶。","authors":"Jianyong Liu, Yu Pei, Yingying Huang, Haofei Jiang, Mike Pleass Tita Tadoh, Xixi Huang, Jintao Shen, Xiaoxi Zeng, Bin Zheng, Jingye Pan, Guoqiang Yang, Haihong Jiang, Limin Chen and Yunlong Zhou","doi":"10.1039/D4TB01456A","DOIUrl":null,"url":null,"abstract":"<p >Edema of the prepuce is a common clinical complication following circumcision in urology. Nevertheless, the principal method of gauze wrapping in clinical practice has not yet been demonstrated to be an effective solution to the problem of postoperative edema. It is therefore evident that the development of functional dressings for the treatment of post-circumcision edema has considerable potential for both clinical application and translational significance. The objective of this study was to develop a single-component bioactive oligopeptide hydrogel dressing with favorable anti-inflammatory, pro-angiogenesis properties and biosafety for the treatment of edema following prepuce surgery. A hexapeptide (Ac-FFFGHK-OH) hydrogelator was designed and synthesized through the N-terminal modification of a human-derived glycine–histidine–lysine tripeptide (GHK) with an <em>N</em>-acetylated phenylalanine tripeptide (Ac-FFF). The bioactive Ac-FFFGHK-OH hydrogel can be prepared through a facile self-assembly method. Furthermore, a novel experimental animal model of post-circumcision edema in Sprague–Dawley rats was constructed and the <em>in vivo</em> anti-edema effect of the Ac-FFFGHK-OH hydrogel was confirmed. The mechanism of action in relieving edema was also investigated by <em>in vitro</em> cell experiments. This work may inspire alternative thinking for the development of mono-component bioactive oligopeptide hydrogels for the treatment of edema in various diseases.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":null,"pages":null},"PeriodicalIF":6.1000,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A self-assembling bioactive oligopeptide hydrogel for the treatment of edema following prepuce surgery†\",\"authors\":\"Jianyong Liu, Yu Pei, Yingying Huang, Haofei Jiang, Mike Pleass Tita Tadoh, Xixi Huang, Jintao Shen, Xiaoxi Zeng, Bin Zheng, Jingye Pan, Guoqiang Yang, Haihong Jiang, Limin Chen and Yunlong Zhou\",\"doi\":\"10.1039/D4TB01456A\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >Edema of the prepuce is a common clinical complication following circumcision in urology. 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Furthermore, a novel experimental animal model of post-circumcision edema in Sprague–Dawley rats was constructed and the <em>in vivo</em> anti-edema effect of the Ac-FFFGHK-OH hydrogel was confirmed. The mechanism of action in relieving edema was also investigated by <em>in vitro</em> cell experiments. 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引用次数: 0
摘要
包皮水肿是泌尿外科包皮环切术后常见的临床并发症。然而,临床实践中主要的纱布包扎方法尚未证明能有效解决术后水肿问题。因此,开发治疗包皮环切术后水肿的功能性敷料显然具有相当大的临床应用潜力和转化意义。本研究的目的是开发一种单组分生物活性低聚肽水凝胶敷料,该敷料具有良好的抗炎、促进血管生成的特性和生物安全性,可用于治疗包皮手术后的水肿。通过对源自人类的甘氨酸-组氨酸-赖氨酸三肽(GHK)与 N-乙酰化苯丙氨酸三肽(Ac-FFF)进行 N 端修饰,设计并合成了六肽(Ac-FFFGHK-OH)水凝胶。生物活性 Ac-FFFGHK-OH 水凝胶可通过简便的自组装方法制备。此外,还构建了一种新型的 Sprague-Dawley 大鼠包皮环切后水肿实验动物模型,并证实了 Ac-FFFGHK-OH 水凝胶的体内抗水肿作用。此外,还通过体外细胞实验研究了水凝胶缓解水肿的作用机制。这项工作可能会为开发单组分生物活性寡肽水凝胶治疗各种疾病的水肿提供另一种思路。
A self-assembling bioactive oligopeptide hydrogel for the treatment of edema following prepuce surgery†
Edema of the prepuce is a common clinical complication following circumcision in urology. Nevertheless, the principal method of gauze wrapping in clinical practice has not yet been demonstrated to be an effective solution to the problem of postoperative edema. It is therefore evident that the development of functional dressings for the treatment of post-circumcision edema has considerable potential for both clinical application and translational significance. The objective of this study was to develop a single-component bioactive oligopeptide hydrogel dressing with favorable anti-inflammatory, pro-angiogenesis properties and biosafety for the treatment of edema following prepuce surgery. A hexapeptide (Ac-FFFGHK-OH) hydrogelator was designed and synthesized through the N-terminal modification of a human-derived glycine–histidine–lysine tripeptide (GHK) with an N-acetylated phenylalanine tripeptide (Ac-FFF). The bioactive Ac-FFFGHK-OH hydrogel can be prepared through a facile self-assembly method. Furthermore, a novel experimental animal model of post-circumcision edema in Sprague–Dawley rats was constructed and the in vivo anti-edema effect of the Ac-FFFGHK-OH hydrogel was confirmed. The mechanism of action in relieving edema was also investigated by in vitro cell experiments. This work may inspire alternative thinking for the development of mono-component bioactive oligopeptide hydrogels for the treatment of edema in various diseases.
期刊介绍:
Journal of Materials Chemistry A, B & C cover high quality studies across all fields of materials chemistry. The journals focus on those theoretical or experimental studies that report new understanding, applications, properties and synthesis of materials. Journal of Materials Chemistry A, B & C are separated by the intended application of the material studied. Broadly, applications in energy and sustainability are of interest to Journal of Materials Chemistry A, applications in biology and medicine are of interest to Journal of Materials Chemistry B, and applications in optical, magnetic and electronic devices are of interest to Journal of Materials Chemistry C.Journal of Materials Chemistry B is a Transformative Journal and Plan S compliant. Example topic areas within the scope of Journal of Materials Chemistry B are listed below. This list is neither exhaustive nor exclusive:
Antifouling coatings
Biocompatible materials
Bioelectronics
Bioimaging
Biomimetics
Biomineralisation
Bionics
Biosensors
Diagnostics
Drug delivery
Gene delivery
Immunobiology
Nanomedicine
Regenerative medicine & Tissue engineering
Scaffolds
Soft robotics
Stem cells
Therapeutic devices