Thi Minh Chau Ngo, Antonella Santona, Phuong Anh Ton Nu, Le Chi Cao, Giang Tran Thi, Thi Bich Thao Do, Thi Ngoc Thuy Ha, Tiep Vo Minh, Phuoc Vinh Nguyen, Dong Duong Ton That, My Nguyen Thi Tra, Duc Bui Van
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引用次数: 0
摘要
脑癣毛癣菌（Trichophyton mentagrophytes complex）是一类皮癣真菌，可引起各种皮肤感染。该复合菌种的耐药性不断增加，尤其是吲哚癣菌对特比萘芬的耐药性，这是皮肤科医生在临床实践中的一个主要问题。本研究全面分析了从越南顺化市患者身上分离出的嗜皮毛癣菌复合菌株，重点研究了它们的表型和遗传特征、抗真菌药敏谱和分子流行病学。从皮真菌病培养样本中分离出的嗜角质真菌通过形态和表型进行了鉴定，并通过 ITS 测序和系统发育分析确认了菌种和基因型。抗真菌药敏试验评估了这些真菌对伊曲康唑、伏立康唑和特比萘芬的药敏性。24%（n=27/114）的浅表真菌病从表型上看是由曼地夫线虫复合体分离物引起的。主要是基因型II*的T.interigitale占主导地位（44.4%），其次是基因型III*的T.mentagrophytes（22.2%）、T.indotineae（14.8%）、T.tonsurans（11.2%）和T.mentagrophytes（7.4%）。虽然所有分离株都对伊曲康唑和伏立康唑敏感，但一半的吲哚癣菌分离株对特比萘芬表现出抗药性，这与 SQLE 蛋白中的 Phe397Leu 突变有关。这项研究强调了越南存在对特比萘芬耐药的吲哚癣菌分离株，强调了调查皮癣菌耐药性并在临床实践中采取有效措施的必要性。
Detection of terbinafine-resistant Trichophyton indotineae isolates within the Trichophyton mentagrophytes species complex isolated from patients in Hue City, Vietnam: A comprehensive analysis.
The Trichophyton mentagrophytes complex comprises a group of dermatophyte fungi responsible for various dermatological infections. The increasing drug resistance of this species complex, especially terbinafine resistance of Trichophyton indotineae, is a major concern in dermatologist practice. This study provides a comprehensive analysis of T. mentagrophytes complex strains isolated from patients in Hue City, Vietnam, focusing on their phenotypic and genetic characteristics, antifungal susceptibility profiles, and molecular epidemiology. Keratinophilic fungi from dermatophytosis culture samples were identified morphologically and phenotypically, with species and genotypes confirmed by internal transcribed spacer sequencing and phylogenetic analysis. Antifungal susceptibility testing was carried out to evaluate their susceptibility to itraconazole, voriconazole, and terbinafine. The 24% (n = 27/114) of superficial mycoses were phenotypically attributed to T. mentagrophytes complex isolates. Trichophyton interdigitale, mainly genotype II*, was predominant (44.4%), followed by T. mentagrophytes genotype III* (22.2%), T. indotineae (14.8%), T. tonsurans (11.2%), and T. mentagrophytes (7.4%). While all isolates were susceptible to itraconazole and voriconazole, half of T. indotineae isolates exhibited resistance to terbinafine, linked to the Phe397Leu mutation in the SQLE protein. This study highlighted the presence of terbinafine-resistant T. indotineae isolates in Vietnam, emphasizing the need to investigate dermatophyte drug resistance and implement effective measures in clinical practice.
期刊介绍：
Medical Mycology is a peer-reviewed international journal that focuses on original and innovative basic and applied studies, as well as learned reviews on all aspects of medical, veterinary and environmental mycology as related to disease. The objective is to present the highest quality scientific reports from throughout the world on divergent topics. These topics include the phylogeny of fungal pathogens, epidemiology and public health mycology themes, new approaches in the diagnosis and treatment of mycoses including clinical trials and guidelines, pharmacology and antifungal susceptibilities, changes in taxonomy, description of new or unusual fungi associated with human or animal disease, immunology of fungal infections, vaccinology for prevention of fungal infections, pathogenesis and virulence, and the molecular biology of pathogenic fungi in vitro and in vivo, including genomics, transcriptomics, metabolomics, and proteomics. Case reports are no longer accepted. In addition, studies of natural products showing inhibitory activity against pathogenic fungi are not accepted without chemical characterization and identification of the compounds responsible for the inhibitory activity.
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