Xiaoyan Zhu, Chao Ma, Rina Sa, Yaxuan Wang, Chaohui Zhu, Yajiao Zhao, Juan Luo, Xiaochuan Liu
{"title":"中国人群幽门螺杆菌 CagA 3' 区多态性及其与慢性胃炎的关系。","authors":"Xiaoyan Zhu, Chao Ma, Rina Sa, Yaxuan Wang, Chaohui Zhu, Yajiao Zhao, Juan Luo, Xiaochuan Liu","doi":"10.1099/jmm.0.001880","DOIUrl":null,"url":null,"abstract":"<p><p><b>Introduction</b>. Cytotoxin-associated gene A (CagA) from <i>Helicobacter pylori</i> is highly related to chronic gastritis. Tyrosine phosphorylation of Glu-Pro-Ile-Tyr-Ala (EPIYA) motifs from CagA determines the pathogenicity of <i>H. pylori</i>.<b>Gap statement</b>. The precise amino acid variations surrounding the EPIYA motifs and their correlation with clinical outcomes have been poorly explored.<b>Aim</b>. The purpose of this study was to examine the CagA 3' region polymorphism of <i>H. pylori</i> and its association with chronic gastritis in the Chinese population.<b>Method</b>. A total of 86 <i>cagA</i>-positive <i>H. pylori</i> strains were isolated from patients with chronic gastritis in two different hospitals in Beijing, PR China. Genomic DNA was extracted commercial kits, and the <i>cagA</i> 3' variable region of <i>H. pylori</i> was amplified by polymerase chain reaction (PCR). The PCR products were sequenced and analysed using the CLC Sequence Viewer, BioEdit, and WebLogo 3.<b>Results</b>. Two hundred and fifty-nine EPIYA motifs were identified from <i>cagA</i>-positive <i>H. pylori</i> strains. Notably, EPIYA-B exhibited a higher frequency of variation in comparison to EPIYA-A, EPIYA-C, and EPIYA-D. The prevalent sequences for East-Asian-type CagA were QVNK and TIDF, while KVNK and TIDD were most commonly observed for Western-type CagA. The CRPIA motifs of East-Asian-type CagA and Western-type CagA varied at positions 4, 6, 7, 8, and 10. CagA-ABD (73.2%) was the most prevalent type, followed by CagA-ABC (18.6%) and CagA-AB (3.4%). The ratio of CagA-ABD was observed to be higher in cases of chronic non-atrophic gastritis with erosive (NAGE) or chronic atrophic gastritis (AG) compared to chronic non-atrophic gastritis (NAG), and the difference was found to be statistically significant (χ2=59.000/64.000, <i>P</i><0.001).<b>Conclusions</b>. The EPIYA segments of Western-type CagA and East-Asian-type CagA differ significantly and the presence of CagA-ABD may be associated with severe chronic gastritis from this study.</p>","PeriodicalId":94093,"journal":{"name":"Journal of medical microbiology","volume":"73 8","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"CagA 3' region polymorphism of <i>Helicobacter pylori</i> and its association with chronic gastritis in the Chinese population.\",\"authors\":\"Xiaoyan Zhu, Chao Ma, Rina Sa, Yaxuan Wang, Chaohui Zhu, Yajiao Zhao, Juan Luo, Xiaochuan Liu\",\"doi\":\"10.1099/jmm.0.001880\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><b>Introduction</b>. Cytotoxin-associated gene A (CagA) from <i>Helicobacter pylori</i> is highly related to chronic gastritis. Tyrosine phosphorylation of Glu-Pro-Ile-Tyr-Ala (EPIYA) motifs from CagA determines the pathogenicity of <i>H. pylori</i>.<b>Gap statement</b>. The precise amino acid variations surrounding the EPIYA motifs and their correlation with clinical outcomes have been poorly explored.<b>Aim</b>. The purpose of this study was to examine the CagA 3' region polymorphism of <i>H. pylori</i> and its association with chronic gastritis in the Chinese population.<b>Method</b>. A total of 86 <i>cagA</i>-positive <i>H. pylori</i> strains were isolated from patients with chronic gastritis in two different hospitals in Beijing, PR China. Genomic DNA was extracted commercial kits, and the <i>cagA</i> 3' variable region of <i>H. pylori</i> was amplified by polymerase chain reaction (PCR). The PCR products were sequenced and analysed using the CLC Sequence Viewer, BioEdit, and WebLogo 3.<b>Results</b>. Two hundred and fifty-nine EPIYA motifs were identified from <i>cagA</i>-positive <i>H. pylori</i> strains. Notably, EPIYA-B exhibited a higher frequency of variation in comparison to EPIYA-A, EPIYA-C, and EPIYA-D. The prevalent sequences for East-Asian-type CagA were QVNK and TIDF, while KVNK and TIDD were most commonly observed for Western-type CagA. The CRPIA motifs of East-Asian-type CagA and Western-type CagA varied at positions 4, 6, 7, 8, and 10. CagA-ABD (73.2%) was the most prevalent type, followed by CagA-ABC (18.6%) and CagA-AB (3.4%). The ratio of CagA-ABD was observed to be higher in cases of chronic non-atrophic gastritis with erosive (NAGE) or chronic atrophic gastritis (AG) compared to chronic non-atrophic gastritis (NAG), and the difference was found to be statistically significant (χ2=59.000/64.000, <i>P</i><0.001).<b>Conclusions</b>. The EPIYA segments of Western-type CagA and East-Asian-type CagA differ significantly and the presence of CagA-ABD may be associated with severe chronic gastritis from this study.</p>\",\"PeriodicalId\":94093,\"journal\":{\"name\":\"Journal of medical microbiology\",\"volume\":\"73 8\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of medical microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1099/jmm.0.001880\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of medical microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1099/jmm.0.001880","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
CagA 3' region polymorphism of Helicobacter pylori and its association with chronic gastritis in the Chinese population.
Introduction. Cytotoxin-associated gene A (CagA) from Helicobacter pylori is highly related to chronic gastritis. Tyrosine phosphorylation of Glu-Pro-Ile-Tyr-Ala (EPIYA) motifs from CagA determines the pathogenicity of H. pylori.Gap statement. The precise amino acid variations surrounding the EPIYA motifs and their correlation with clinical outcomes have been poorly explored.Aim. The purpose of this study was to examine the CagA 3' region polymorphism of H. pylori and its association with chronic gastritis in the Chinese population.Method. A total of 86 cagA-positive H. pylori strains were isolated from patients with chronic gastritis in two different hospitals in Beijing, PR China. Genomic DNA was extracted commercial kits, and the cagA 3' variable region of H. pylori was amplified by polymerase chain reaction (PCR). The PCR products were sequenced and analysed using the CLC Sequence Viewer, BioEdit, and WebLogo 3.Results. Two hundred and fifty-nine EPIYA motifs were identified from cagA-positive H. pylori strains. Notably, EPIYA-B exhibited a higher frequency of variation in comparison to EPIYA-A, EPIYA-C, and EPIYA-D. The prevalent sequences for East-Asian-type CagA were QVNK and TIDF, while KVNK and TIDD were most commonly observed for Western-type CagA. The CRPIA motifs of East-Asian-type CagA and Western-type CagA varied at positions 4, 6, 7, 8, and 10. CagA-ABD (73.2%) was the most prevalent type, followed by CagA-ABC (18.6%) and CagA-AB (3.4%). The ratio of CagA-ABD was observed to be higher in cases of chronic non-atrophic gastritis with erosive (NAGE) or chronic atrophic gastritis (AG) compared to chronic non-atrophic gastritis (NAG), and the difference was found to be statistically significant (χ2=59.000/64.000, P<0.001).Conclusions. The EPIYA segments of Western-type CagA and East-Asian-type CagA differ significantly and the presence of CagA-ABD may be associated with severe chronic gastritis from this study.
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