移动中的脂质

IF 12.9 1区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Nature chemical biology Pub Date : 2024-08-20 DOI:10.1038/s41589-024-01725-y

Majda Bratovič

引用次数: 0

摘要

细胞器之间的脂质转运对许多生物过程都至关重要，通常通过脂质转移蛋白（LTPs）在膜接触部位进行。由于目前跟踪细胞内脂质转运的大多数方法都是间接的，因此研究这一过程具有挑战性，而且目前还不清楚体外获得的见解是否可以转化到细胞环境中。为了解决这个问题，Luan、Liang 等人开发了一种方法，用于成像内质网和质膜之间的脂质运输。该方法基于对 IMPACT（通过反磷脂酰化用可点击的醇成像 PLD 活性）技术的重新利用，该技术最初是为可视化磷脂酶 D (PLD) 活性而开发的。PLD 使用氧代-反式环辛烯伯醇探针进行反磷脂酰化，从而在质膜上形成含氧代-反式环辛烯的磷脂酰醇脂质。这些脂质通过与含氟四嗪-BODIPY试剂的生物正交反应进行标记，从而获得荧光磷脂酰醇脂质，并转运到内质网，可通过荧光成像进行观察。研究小组随后使用短干扰 RNA（siRNA）三重敲除了扩展突触素（E-Syts），结果表明这些 LTPs 参与了 IMPACT 脂质的细胞器间转运。此外，在内质网-线粒体接触点使用基于E-Syt的人工系链可将转运重定向至线粒体。研究小组还能在体外重建两个脂质体之间的 IMPACT 脂质运输，证实它需要 E-Syts。基于 IMPACT 的方法可用于监测其他 LTPs 的活性，并获得细胞内脂质运输的新见解：ACS Chem.https://doi.org/10.1021/acschembio.4c00345 (2024)

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来源期刊

Nature chemical biology 生物-生化与分子生物学

CiteScore

23.90

自引率

1.40%

发文量

238

审稿时长

12 months

期刊介绍： Nature Chemical Biology stands as an esteemed international monthly journal, offering a prominent platform for the chemical biology community to showcase top-tier original research and commentary. Operating at the crossroads of chemistry, biology, and related disciplines, chemical biology utilizes scientific ideas and approaches to comprehend and manipulate biological systems with molecular precision. The journal embraces contributions from the growing community of chemical biologists, encompassing insights from chemists applying principles and tools to biological inquiries and biologists striving to comprehend and control molecular-level biological processes. We prioritize studies unveiling significant conceptual or practical advancements in areas where chemistry and biology intersect, emphasizing basic research, especially those reporting novel chemical or biological tools and offering profound molecular-level insights into underlying biological mechanisms. Nature Chemical Biology also welcomes manuscripts describing applied molecular studies at the chemistry-biology interface due to the broad utility of chemical biology approaches in manipulating or engineering biological systems. Irrespective of scientific focus, we actively seek submissions that creatively blend chemistry and biology, particularly those providing substantial conceptual or methodological breakthroughs with the potential to open innovative research avenues. The journal maintains a robust and impartial review process, emphasizing thorough chemical and biological characterization.