重组人粒细胞集落刺激因子 pegfilgrastim 对雌雄鹿鼠(Peromyscus maniculatus bairdii)中性粒细胞数量的不同影响。

IF 2.8 3区 医学 Q2 PHARMACOLOGY & PHARMACY
J P Strydom, Linda Brand, Francois P Viljoen, De Wet Wolmarans
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引用次数: 0

摘要

背景:越来越多的研究表明,炎症过程(包括中性粒细胞-淋巴细胞比率(NLR)的改变)与精神疾病的病理生理学有关。鹿鼠(Peromyscus maniculatus bairdii)因其强迫行为的自然表现而被广泛研究。为了了解先天性免疫过程和适应性免疫过程在该模型中的参与情况,我们旨在研究聚乙二醇化重组人粒细胞集落刺激因子(g-CSF)类似物 pegfilgrastim 对雄性和雌性鹿小鼠 NLR 的影响:简言之,54 只鹿小鼠(雌雄各半)接受对照组或 pegfilgrastim(0.1 或 1 毫克/千克)(每组 18 只)的单次注射。每组 6 只小鼠(雌雄各 3 只)在注射后第 2、4 和 7 天被安乐死,采集其血液并计算 NLR。数据分析采用普通的三方方差分析,然后进行Bonferroni事后检验:结果:无论剂量大小,在测试的第四天和第七天,培非格司亭都能使雌雄小鼠的NLR值升高。然而,与暴露于相同剂量的雄性小鼠相比,暴露于较高剂量的雌性小鼠在任何时间都表现出明显较高的 NLR 值:结论:这项研究得出的数据强调了培吉瑞司群对剂量和性别的重要特异性,雌性小鼠仅在服用高剂量培吉瑞司群后才表现出 NLR 的升高。由于雌雄鹿小鼠的先天免疫成分对 g-CSF 刺激的敏感性不同,我们的研究结果为进一步研究鹿小鼠的性别特异性免疫过程提供了有用的依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Differential impact of pegfilgrastim, a recombinant human granulocyte colony stimulating factor, on the neutrophil count of male and female deer mice (Peromyscus maniculatus bairdii).

Background: An increasing body of research implicates inflammatory processes, including alterations in the neutrophil-lymphocyte ratio (NLR), in the pathophysiology of psychiatric illness. The deer mouse (Peromyscus maniculatus bairdii) is commonly studied for its naturalistic expression of compulsive-like behaviour. Towards future efforts to gain an understanding of how innate and adaptive immune processes might be involved in this model, we aimed to study the effects of pegfilgrastim, a pegylated recombinant human granulocyte colony-stimulating factor (g-CSF) analogue, on the NLR of both male and female deer mice.

Methods: Briefly, 54 deer mice (equally distributed between sexes) were exposed to a single injection with either control or pegfilgrastim (0.1 or 1 mg/kg) (n = 18 per group). Six mice of each group (three per sex) were euthanized on days two, four and seven post-administration, their blood collected and the NLR calculated. Data were analysed by means of ordinary three-way ANOVA, followed by Bonferroni post-hoc testing.

Results: Irrespective of dose, pegfilgrastim resulted in higher NLR values in mice of both sexes at days four and seven of testing. However, female mice exposed to the higher dose, presented with significantly higher NLR values irrespective of time, compared to male mice exposed to the same.

Conclusion: The data generated from this work highlight important dose- and sex-specific aspects of pegfilgrastim with female mice showing heighted elevation of the NLR in response to high-dose pegfilgrastim administration only. Since the innate immune components of male and female deer mice is differentially sensitive to g-CSF stimulation, our results provide a useful basis for further study of sex-specific immunological processes in deer mice.

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来源期刊
BMC Pharmacology & Toxicology
BMC Pharmacology & Toxicology PHARMACOLOGY & PHARMACYTOXICOLOGY&nb-TOXICOLOGY
CiteScore
4.80
自引率
0.00%
发文量
87
审稿时长
12 weeks
期刊介绍: BMC Pharmacology and Toxicology is an open access, peer-reviewed journal that considers articles on all aspects of chemically defined therapeutic and toxic agents. The journal welcomes submissions from all fields of experimental and clinical pharmacology including clinical trials and toxicology.
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