右美托咪定可诱导肝细胞癌患者外周血中的 B 淋巴细胞分泌 IL-10

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Miaomiao Qin , Yining Chen , Xinxin Wang , Xiaobao Zhang , Xiongxiong Pan
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引用次数: 0

摘要

背景/目的 研究肝细胞癌(HCC)患者外周血B淋巴细胞亚群的分布,并评估右美托咪定(DEX)对HCC患者体外B淋巴细胞分化的影响。方法 收集 HCC 组和健康组的外周血单核细胞(PBMCs),用流式细胞术(FCM)检测两组外周血 B 淋巴细胞亚群的分布。根据培养基中 DEX 的不同剂量(0 μM、1 μM、2 μM 和 4 μM),将 HCC 组外周血中提取的 B 淋巴细胞分为 D0、D1、D2 和 D4 组。结果与健康组相比,HCC 组 CD19 + CD73 + B 淋巴细胞亚群比例的增加有统计学意义(P<0.05)。在体外培养实验中,各组 B 淋巴细胞凋亡率和 TGF-β 表达比例差异无统计学意义;与对照组相比,D1、D2 和 D4 组 B 淋巴细胞表达 IL-10 的比例显著增加(P<0.05)。结论 HCC 患者外周血中 CD19 + CD73 + B 淋巴细胞亚群升高;DEX 可能通过促进 HCC 患者外周血 B 淋巴细胞分泌 IL-10 而产生免疫抑制作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Dexmedetomidine induces IL-10 secretion by B lymphocytes in the peripheral blood of patients with hepatocellular carcinoma

Background/aim

To investigate the distribution of subpopulations of peripheral blood B lymphocytes in individuals with hepatocellular carcinoma (HCC), and to evaluate the effect of dexmedetomidine (DEX) on B lymphocyte differentiation in patients with HCC in vitro.

Methods

Peripheral blood mononuclear cells (PBMCs) were collected from the HCC group and the healthy group, and the distribution of peripheral blood B-lymphocyte subpopulations in the two groups was examined by Flow Cytometry (FCM). B lymphocytes extracted from the peripheral blood of the HCC group were divided into D0, D1, D2 and D4 groups according to the different dose of DEX in the culture medium (0 μM, 1 μM, 2 μM and 4 μM). After 72 h of in vitro culture, FCM was used to detect differences in the percentage of apoptotic B lymphocytes and the percentage of B lymphocytes that can express interleukin 10(IL-10) and transforming growth factor-β (TGF-β) in each group.

Results

In contrast to the healthy group, the HCC group exhibited a statistically significant increase in the proportion of CD19 + CD73 + B lymphocyte subpopulation (P<0.05). In the in vitro culture experiment, the differences in apoptosis of B lymphocytes and the percentage of TGF-β expression in each group were not statistically significant; When compared to the control group, there was a significant increase in the percentage of B lymphocytes expressing IL-10 across the D1, D2, and D4 groups (P<0.05).

Conclusion

The peripheral blood of HCC patients is characterized by an elevated presence of CD19 + CD73 + B lymphocyte subpopulations; DEX may have an immunosuppressive effect by promoting IL-10 secretion from peripheral blood B lymphocytes of HCC patients.

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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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