镰状细胞贫血症白细胞的吞噬能力和氧化呼吸爆发活动受损

IF 1.5 Q2 MEDICINE, GENERAL & INTERNAL
{"title":"镰状细胞贫血症白细胞的吞噬能力和氧化呼吸爆发活动受损","authors":"","doi":"10.1016/j.jtumed.2024.07.010","DOIUrl":null,"url":null,"abstract":"<div><h3>Objectives</h3><p>This case-control study investigated the mode of leukocyte function in sickle cell anemia (SCA) to delineate the underlying immunopathology for early diagnosis and mitigate the increased bacterial infection risk in this patient population.</p></div><div><h3>Method</h3><p>In total, 90 participants comprising 24 hemoglobin (Hb)-AA, 22 Hb-AS, 23 steady state Hb-SS and 21 vaso-occlusive crisis state Hb-SS subjects were recruited for this study. The subjects were further divided into the following six groups: Hb-AA and Hb-AS subjects as control groups, Hb-SS subjects at steady state, Hb-SS subjects in a vaso-occlusive crisis state, Hb-SS subjects undergoing medication (Meds), and Hb-SS subjects undergoing medication plus blood transfusion (Meds/BT) group, respectively. Hematological analysis, Hb electrophoresis, leukocyte ratios, and leukocyte functional assays were assessed with standard methods, and interleukin 8 (IL-8) and L-selectin levels were evaluated using enzyme-linked immunosorbent assays.</p></div><div><h3>Results</h3><p>Total leukocyte and monocyte counts were increased in the Hb-SS groups compared to the control groups. However, the Hb-SS groups had lower lymphocyte counts than the other groups (p &lt; 0.005). Leukocyte viability was increased in the SCA groups, while phagocytic activities and oxidative respiratory burst were both reduced in the SCA groups (p &lt; 0.005). Increased IL-8 levels were observed in all SCA groups (p &lt; 0.05), whereas L-selectin levels of the Hb-SS steady and Hb-SS on Meds groups were decreased compared to the other groups (p &lt; 0.05). The neutrophil-to-lymphocyte ratio, monocyte-to-lymphocyte ratio, and platelet-to-lymphocyte ratio were higher in the SCA groups than the control groups (p &lt; 0.05).</p></div><div><h3>Conclusion</h3><p>Impaired leukocyte phagocytic and oxidative respiratory burst activities constitute altered leukocyte function in SCA, which can increase their susceptibility to infections and the risk of mortality, especially during the crisis state. Novel therapeutic approaches can be tailored specifically to enhance these leukocyte functions and mitigate the increased infection risk in SCA.</p></div>","PeriodicalId":46806,"journal":{"name":"Journal of Taibah University Medical Sciences","volume":null,"pages":null},"PeriodicalIF":1.5000,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1658361224000726/pdfft?md5=a76d6a8937fd2774df903f9c1c2071dd&pid=1-s2.0-S1658361224000726-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Impaired phagocytosis and oxidative respiratory burst activity in sickle cell anemia leukocytes\",\"authors\":\"\",\"doi\":\"10.1016/j.jtumed.2024.07.010\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objectives</h3><p>This case-control study investigated the mode of leukocyte function in sickle cell anemia (SCA) to delineate the underlying immunopathology for early diagnosis and mitigate the increased bacterial infection risk in this patient population.</p></div><div><h3>Method</h3><p>In total, 90 participants comprising 24 hemoglobin (Hb)-AA, 22 Hb-AS, 23 steady state Hb-SS and 21 vaso-occlusive crisis state Hb-SS subjects were recruited for this study. The subjects were further divided into the following six groups: Hb-AA and Hb-AS subjects as control groups, Hb-SS subjects at steady state, Hb-SS subjects in a vaso-occlusive crisis state, Hb-SS subjects undergoing medication (Meds), and Hb-SS subjects undergoing medication plus blood transfusion (Meds/BT) group, respectively. Hematological analysis, Hb electrophoresis, leukocyte ratios, and leukocyte functional assays were assessed with standard methods, and interleukin 8 (IL-8) and L-selectin levels were evaluated using enzyme-linked immunosorbent assays.</p></div><div><h3>Results</h3><p>Total leukocyte and monocyte counts were increased in the Hb-SS groups compared to the control groups. However, the Hb-SS groups had lower lymphocyte counts than the other groups (p &lt; 0.005). Leukocyte viability was increased in the SCA groups, while phagocytic activities and oxidative respiratory burst were both reduced in the SCA groups (p &lt; 0.005). Increased IL-8 levels were observed in all SCA groups (p &lt; 0.05), whereas L-selectin levels of the Hb-SS steady and Hb-SS on Meds groups were decreased compared to the other groups (p &lt; 0.05). The neutrophil-to-lymphocyte ratio, monocyte-to-lymphocyte ratio, and platelet-to-lymphocyte ratio were higher in the SCA groups than the control groups (p &lt; 0.05).</p></div><div><h3>Conclusion</h3><p>Impaired leukocyte phagocytic and oxidative respiratory burst activities constitute altered leukocyte function in SCA, which can increase their susceptibility to infections and the risk of mortality, especially during the crisis state. Novel therapeutic approaches can be tailored specifically to enhance these leukocyte functions and mitigate the increased infection risk in SCA.</p></div>\",\"PeriodicalId\":46806,\"journal\":{\"name\":\"Journal of Taibah University Medical Sciences\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2024-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S1658361224000726/pdfft?md5=a76d6a8937fd2774df903f9c1c2071dd&pid=1-s2.0-S1658361224000726-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Taibah University Medical Sciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1658361224000726\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MEDICINE, GENERAL & INTERNAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Taibah University Medical Sciences","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1658361224000726","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MEDICINE, GENERAL & INTERNAL","Score":null,"Total":0}
引用次数: 0

摘要

本病例对照研究调查了镰状细胞性贫血(SCA)患者的白细胞功能模式,以确定潜在的免疫病理,从而进行早期诊断并降低该患者群体中增加的细菌感染风险。本研究共招募了 90 名受试者,包括 24 名血红蛋白(Hb)-AA 受试者、22 名血红蛋白-AS 受试者、23 名处于稳定状态的 Hb-SS 受试者和 21 名处于血管闭塞危象状态的 Hb-SS 受试者。受试者被进一步分为以下六组:Hb-AA 和 Hb-AS 受试者作为对照组、稳定状态 Hb-SS 受试者、血管闭塞危象状态 Hb-SS 受试者、接受药物治疗(Meds)的 Hb-SS 受试者和接受药物治疗加输血(Meds/BT)的 Hb-SS 受试者。结果与对照组相比,Hb-SS 组的白细胞总数和单核细胞计数增加。然而,Hb-SS 组的淋巴细胞计数低于其他组(p < 0.005)。SCA 组的白细胞活力增加,而 SCA 组的吞噬活性和氧化呼吸爆发均降低(p < 0.005)。在所有 SCA 组中均观察到 IL-8 水平升高(p <0.05),而 Hb-SS 稳定组和 Hb-SS 服药组的 L 选择素水平与其他组相比有所降低(p <0.05)。结论白细胞吞噬和氧化呼吸爆发活动受损构成了 SCA 中白细胞功能的改变,这会增加他们对感染的易感性和死亡风险,尤其是在危急状态下。新的治疗方法可以有针对性地增强这些白细胞功能,减轻 SCA 感染风险的增加。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Impaired phagocytosis and oxidative respiratory burst activity in sickle cell anemia leukocytes

Objectives

This case-control study investigated the mode of leukocyte function in sickle cell anemia (SCA) to delineate the underlying immunopathology for early diagnosis and mitigate the increased bacterial infection risk in this patient population.

Method

In total, 90 participants comprising 24 hemoglobin (Hb)-AA, 22 Hb-AS, 23 steady state Hb-SS and 21 vaso-occlusive crisis state Hb-SS subjects were recruited for this study. The subjects were further divided into the following six groups: Hb-AA and Hb-AS subjects as control groups, Hb-SS subjects at steady state, Hb-SS subjects in a vaso-occlusive crisis state, Hb-SS subjects undergoing medication (Meds), and Hb-SS subjects undergoing medication plus blood transfusion (Meds/BT) group, respectively. Hematological analysis, Hb electrophoresis, leukocyte ratios, and leukocyte functional assays were assessed with standard methods, and interleukin 8 (IL-8) and L-selectin levels were evaluated using enzyme-linked immunosorbent assays.

Results

Total leukocyte and monocyte counts were increased in the Hb-SS groups compared to the control groups. However, the Hb-SS groups had lower lymphocyte counts than the other groups (p < 0.005). Leukocyte viability was increased in the SCA groups, while phagocytic activities and oxidative respiratory burst were both reduced in the SCA groups (p < 0.005). Increased IL-8 levels were observed in all SCA groups (p < 0.05), whereas L-selectin levels of the Hb-SS steady and Hb-SS on Meds groups were decreased compared to the other groups (p < 0.05). The neutrophil-to-lymphocyte ratio, monocyte-to-lymphocyte ratio, and platelet-to-lymphocyte ratio were higher in the SCA groups than the control groups (p < 0.05).

Conclusion

Impaired leukocyte phagocytic and oxidative respiratory burst activities constitute altered leukocyte function in SCA, which can increase their susceptibility to infections and the risk of mortality, especially during the crisis state. Novel therapeutic approaches can be tailored specifically to enhance these leukocyte functions and mitigate the increased infection risk in SCA.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Journal of Taibah University Medical Sciences
Journal of Taibah University Medical Sciences MEDICINE, GENERAL & INTERNAL-
CiteScore
3.40
自引率
4.50%
发文量
130
审稿时长
29 days
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信