细胞分裂阻断微核试验在辐射分流和生物剂量测定中的潜力

IF 3.5 Q3 Biochemistry, Genetics and Molecular Biology
G. Tamizh Selvan , P. Venkatachalam
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引用次数: 0

摘要

在细胞分裂受阻的双核细胞中测量微核(MN)已被广泛用作许多辐射生物学应用中的生物标记,特别是生物剂量测定。发生辐射伤亡后,对受辐射人员的医疗管理始于分流和生物剂量测定。细胞分裂受阻微核(CBMN)检测是辐射剂量评估中黄金标准双中心染色体检测的替代方法。近年来,细胞因子阻断微核试验已得到广泛认可,并成为评估职业辐照和意外辐照情况的首选方法。与传统的染色体畸变检测法相比,CBMN 检测法具有成本低、操作简单、剂量评估迅速等优点。使用 PubMed 搜索工具,关键词为 MN、生物模拟、放射治疗,并仅限于人体样本。自 Fenech 和 Morely 开发出该检测方法以来,它作为一种生物标志物在各种应用中经历了许多技术和工艺改革。在这篇综述中,我们简要介绍了 CBMN 检测法在辐射分流和生物剂量学方面的最新发展,重点是:(a) 变量对剂量估算的影响;(b) 基线频率和不同实验室间报告的剂量反应系数值的重要性;(c) 实验室间比较;(d) 其局限性和克服这些局限性的方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Potentials of cytokinesis blocked micronucleus assay in radiation triage and biological dosimetry

The measurement of micronucleus (MN) in the cytokinesis-block arrested binucleated cells has been extensively used as a biomarker in many radiation biology applications in specific biodosimetry. Following radiation casualties, medical management of exposed individuals begins with triage and biological dosimetry. The cytokinesis blocked micronucleus (CBMN) assay is the alternate for the gold standard dicentric chromosome assay in radiation dose assessment. In recent years, the CBMN assay has become well-validated and emerged as a method of choice for evaluating occupational and accidental exposures scenario. It is feasible due to its cost-effective, simple, and rapid dose assessment rather than a conventional chromosome aberration assay. PubMed search tool was used with keywords of MN, biodosimetry, radiotherapy and restricted to human samples. Since Fenech and Morely developed the assay, it has undergone many technical and technological reforms as a biomarker of various applications. In this review, we have abridged recent developments of the CBMN assay in radiation triage and biodosimetry, focusing on (a) the influence of variables on dose estimation, (b) the importance of baseline frequency and reported dose–response coefficient values among different laboratories, (c) inter-laboratory comparison and (d) its limitations and means to overcome them.

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来源期刊
Journal of Genetic Engineering and Biotechnology
Journal of Genetic Engineering and Biotechnology Biochemistry, Genetics and Molecular Biology-Biotechnology
CiteScore
5.70
自引率
5.70%
发文量
159
审稿时长
16 weeks
期刊介绍: Journal of genetic engineering and biotechnology is devoted to rapid publication of full-length research papers that leads to significant contribution in advancing knowledge in genetic engineering and biotechnology and provide novel perspectives in this research area. JGEB includes all major themes related to genetic engineering and recombinant DNA. The area of interest of JGEB includes but not restricted to: •Plant genetics •Animal genetics •Bacterial enzymes •Agricultural Biotechnology, •Biochemistry, •Biophysics, •Bioinformatics, •Environmental Biotechnology, •Industrial Biotechnology, •Microbial biotechnology, •Medical Biotechnology, •Bioenergy, Biosafety, •Biosecurity, •Bioethics, •GMOS, •Genomic, •Proteomic JGEB accepts
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