牛精液冷冻保存介质中添加抗氧化剂双羧乙基二氧化锗对体外评估精子形态功能参数的影响

IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Tairini E Cruz, Luan Sitó-Silva, Rogério A Almeida Filho, Alicio Martins, Fernanda N Marqui, Diego G Souza, Tatiana I U Berton, Camila P Freita-Dell'Aqua, Eunice Oba
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引用次数: 0

摘要

本研究调查了不同浓度的 Ge132(双羧乙基二氧化锗)对冷冻牛精液的影响。将三头公牛的精液集中起来,分成六组,每组都有不同的 Ge132 浓度(0、500 和 1000 μg/mL),每组都在不同的条件下(33°C 孵育 30 分钟(D:D0、D500 和 D1000),另一组立即冷却至 4°C(R:R0-对照组;R500 和 R1000))。解冻后的精液通过 CASA 和流式细胞仪评估精子特性。结果显示,与高浓度 Ge132 相比,不含 Ge132 的立即冷却组精子活力更好。高浓度 Ge132 组和一些对照组的总活力值在 5 分钟和 60 分钟后下降。线性度随着 1000 μg/mL Ge132 浓度的升高而增加,而直线度在多个组的不同时刻存在差异。对照组和某些 Ge132 组的膜完整性更高。没有 Ge132 的情况下,O2 生成量较低。氧化应激诱导后,脂质过氧化强度随花生四烯酸的增加而增加,但 D1000 的过氧化程度低于 R0。总之,Ge132 似乎能保护精子在受到氧化应激时免受 PLM 的影响,因为即使在高浓度下,它也能维持精子的新陈代谢。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of antioxidant Bis-carboxyethyl germanium sesquioxide added to bovine semen cryopreservation medium on in vitro assessed morphofunctional sperm parameters.

This study investigated the impact of various Ge132 (Bis-carboxyethyl germanium sesquioxide) concentrations on frozen bovine semen. Ejaculates from three bulls were pooled and divided into six groups, each one with different Ge132 concentrations (0, 500, and 1000 μg/mL) and each group was incubated in different conditions (33°C for 30 min (D: D0, D500, and D1000), and the other was immediately cooled to 4°C (R: R0-control; R500 and R1000)). Thawed semen was evaluated for sperm characteristics by CASA and flow cytometer. Results showed better motility in the immediate cooling group without Ge132 compared with high Ge132 concentrations. Values for total motility dropped after 5 and 60 min in groups with high Ge132 levels and some control groups. Linearity increased with 1000 μg/mL Ge132, while straightness differed between moments in multiple groups. Membrane integrity was higher in a control group and certain Ge132 groups. Lower O2 - generation occurred without Ge132. After oxidative stress induction, lipid peroxidation intensity increased with arachidonic acid, but D1000 had lower peroxidation than R0. Overall, Ge132 appears to have provided protection against PLM when subjected to oxidative stress, since even at high concentrations it maintained sperm metabolism.

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来源期刊
Reproduction in Domestic Animals
Reproduction in Domestic Animals 农林科学-奶制品与动物科学
CiteScore
3.00
自引率
5.90%
发文量
238
审稿时长
4-8 weeks
期刊介绍: The journal offers comprehensive information concerning physiology, pathology, and biotechnology of reproduction. Topical results are currently published in original papers, reviews, and short communications with particular attention to investigations on practicable techniques. Carefully selected reports, e. g. on embryo transfer and associated biotechnologies, gene transfer, and spermatology provide a link between basic research and clinical application. The journal applies to breeders, veterinarians, and biologists, and is also of interest in human medicine. Interdisciplinary cooperation is documented in the proceedings of the joint annual meetings. Fields of interest: Animal reproduction and biotechnology with special regard to investigations on applied and clinical research.
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