PARK2 是非结核分枝杆菌肺病的易感因素。

IF 5.8 2区 医学 Q1 Medicine
Youngmok Park, Ji Won Hong, Eunsol Ahn, Heon Yung Gee, Young Ae Kang
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引用次数: 0

摘要

背景:与非结核分枝杆菌肺病(NTM-PD)易感性相关的基因特征仍然未知。在这项研究中,我们进行了 RNA 测序,以探索基因表达谱和代表 NTM-PD 特征因素的基因:方法:采集 NTM-PD 患者和健康人(对照组)的外周血样本。通过 RNA 测序确定差异表达基因(DEGs),并进行功能富集和免疫细胞解旋分析:我们招募了 48 名参与者,包括 26 名 NTM-PD 患者(中位年龄 58.0 岁;84.6% 为女性)和 22 名健康对照者(中位年龄 58.5 岁;90.9% 为女性)。与对照组相比,我们在 NTM-PD 组中发现了 21 个上调的 DEGs 和 44 个下调的 DEGs。与对照组相比,NTM-PD 组的基因表达没有受到 NTM 感染的显著影响,免疫细胞的比例也没有差异。然而,通过基因本体(GO)、基因组富集分析(GSEA)和蛋白相互作用(PPI)分析,我们发现 PARK2 是与 NTM-PD 相关的一个关键因素。与泛素化途径相关的 PARK2 基因在 NTM-PD 组中出现下调(折叠变化,- 1.314,P = 0.047)。在治疗取得良好效果后,PARK2 的表达水平保持不变,这表明该基因与宿主的易感性有关,而不是与感染或炎症的结果有关。PARK2 基因诊断 NTM-PD 的接收器操作特征曲线下面积为 0.813(95% 置信区间,0.694-0.932):结论:我们在一组韩国患者中发现了与 NTM-PD 相关的基因特征。PARK2基因是NTM-PD的潜在易感因素。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
PARK2 as a susceptibility factor for nontuberculous mycobacterial pulmonary disease.

Background: The genetic signatures associated with the susceptibility to nontuberculous mycobacterial pulmonary disease (NTM-PD) are still unknown. In this study, we performed RNA sequencing to explore gene expression profiles and represent characteristic factor in NTM-PD.

Methods: Peripheral blood samples were collected from patients with NTM-PD and healthy individuals (controls). Differentially expressed genes (DEGs) were identified by RNA sequencing and subjected to functional enrichment and immune cell deconvolution analyses.

Results: We enrolled 48 participants, including 26 patients with NTM-PD (median age, 58.0 years; 84.6% female), and 22 healthy controls (median age, 58.5 years; 90.9% female). We identified 21 upregulated and 44 downregulated DEGs in the NTM-PD group compared to those in the control group. NTM infection did not have a significant impact on gene expression in the NTM-PD group compared to the control group, and there were no differences in the proportion of immune cells. However, through gene ontology (GO), gene set enrichment analysis (GSEA), and protein-protein interaction (PPI) analysis, we discovered that PARK2 is a key factor associated with NTM-PD. The PARK2 gene, which is linked to the ubiquitination pathway, was downregulated in the NTM-PD group (fold change, - 1.314, P = 0.047). The expression levels of PARK2 remained unaltered after favorable treatment outcomes, suggesting that the gene is associated with host susceptibility rather than with the outcomes of infection or inflammation. The area under the receiver operating characteristic curve for the PARK2 gene diagnosing NTM-PD was 0.813 (95% confidence interval, 0.694-0.932).

Conclusion: We identified the genetic signatures associated with NTM-PD in a cohort of Korean patients. The PARK2 gene presents as a potential susceptibility factor in NTM-PD .

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来源期刊
Respiratory Research
Respiratory Research RESPIRATORY SYSTEM-
CiteScore
9.70
自引率
1.70%
发文量
314
审稿时长
4-8 weeks
期刊介绍: Respiratory Research publishes high-quality clinical and basic research, review and commentary articles on all aspects of respiratory medicine and related diseases. As the leading fully open access journal in the field, Respiratory Research provides an essential resource for pulmonologists, allergists, immunologists and other physicians, researchers, healthcare workers and medical students with worldwide dissemination of articles resulting in high visibility and generating international discussion. Topics of specific interest include asthma, chronic obstructive pulmonary disease, cystic fibrosis, genetics, infectious diseases, interstitial lung diseases, lung development, lung tumors, occupational and environmental factors, pulmonary circulation, pulmonary pharmacology and therapeutics, respiratory immunology, respiratory physiology, and sleep-related respiratory problems.
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