Yanjun Mao, Qinglin Yang, Junhong Liu, Yuxin Fu, Shuaishuai Zhou, Jiayan Liu, Linlin Ying, Yao Li
{"title":"槲皮素提高断奶仔猪的生长性能并降低腹泻发生率及其作用机制","authors":"Yanjun Mao, Qinglin Yang, Junhong Liu, Yuxin Fu, Shuaishuai Zhou, Jiayan Liu, Linlin Ying, Yao Li","doi":"10.1155/2024/5632260","DOIUrl":null,"url":null,"abstract":"<p><p>This study aimed to investigate the mechanism of quercetin increasing growth performance and decreasing incidence of diarrhea in weaned piglets. Forty-eight Duroc × Landrace × Large White weaned piglets with similar body weight (7.48 ± 0.20 kg, 28 days of age) were randomly divided into four treatments (control, 250 mg/kg quercetin, 500 mg/kg quercetin, and 750 mg/kg quercetin treatments) and fed with basal diet or experimental diet supplemented with quercetin. Performance, diarrhea rate and index, and content of serum anti-inflammatory factors were determined and calculated in weaned piglets; colonic flora and signaling pathways related to anti-inflammation were measured using 16S rDNA sequencing and RNA-seq, respectively. The results showed that compared with control, feed-to-gain ratio and content of serum interferon gamma (IFN-<i>γ</i>) were significantly decreased in the 500 and 750 mg/kg quercetin treatments (<i>P</i> < 0.05); quercetin significantly decreased diarrhea rate and diarrhea index (<i>P</i> < 0.05) and significantly increased the content of serum transforming growth factor (TGF-<i>β</i>) in weaned piglets (<i>P</i> < 0.05); the content of serum NF-<i>κ</i>B was significantly decreased in the 750 mg/kg quercetin treatment (<i>P</i> < 0.05); moreover, quercetin significantly increased diversity of colonic flora (<i>P</i> < 0.05), and at the phylum level, the relative abundance of Actinobacteria in the 500 and 750 mg/kg treatments was significantly increased (<i>P</i> < 0.05), and the relative abundance of Proteobacteria in the three quercetin treatments were significantly decreased (<i>P</i> < 0.05) in the colon of weaned piglets; at the genus level, the relative abundance of <i>Clostridium-sensu-stricto-1</i>, <i>Turicibacter</i>, <i>unclassified_f_Lachnospiraceae</i>, <i>Phascolarctobacterium</i>, and <i>Family_XIII _AD3011_group</i> was significantly increased (<i>P</i> < 0.05); the relative abundance of <i>Subdollgranulum</i> and <i>Blautia</i> was significantly decreased in the 500 and 750 mg/kg treatments (<i>P</i> < 0.05); the relative abundance of <i>Eschericha-Shigella</i>, <i>Terrisporobacter</i>, and <i>Eubacterium-coprostanoligenes</i> was significantly increased (<i>P</i> < 0.05); the relative abundance of <i>Streptocococcus</i>, <i>Sarcina</i>, <i>Staphylococcus</i>, and <i>Ruminococcaceae_UCG-008</i> was significantly decreased in the three quercetin treatments (<i>P</i> < 0.05); the relative abundance of <i>Ruminococcaceae_UCG_014</i> was significantly increased in the 250 mg/kg quercetin treatment in the colon of weaned piglets (<i>P</i> < 0.05). The results of Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that differentially expressed genes (DEGs) from the quercetin treatments were significantly enriched in nuclear transcription factor-<i>κ</i>B (NF-<i>κ</i>B) signal pathway (<i>P</i> < 0.05); mRNA expression of tumor necrosis factor-<i>α</i> (TNF-<i>α</i>), interleukin-1R1 (IL-1R1), conserved helix-loop-helix ubiquitous kinase (CHUK), toll-like receptor 4 (TLR4), and IL-1<i>β</i> from quercetin treatments were significantly decreased in colonic mucosa of weaned piglets (<i>P</i> < 0.05). In summary, quercetin increased feed conversion ratio and decreased diarrhea through regulating NF-<i>κ</i>B signaling pathway, controlling the balance between anti-inflammatory and proinflammatory factors, and modulating intestinal flora, thus promoting the absorption of nutrients in weaned piglets. These results provided the theoretical foundation for applying quercetin in preventing weaning piglets' diarrhea and animal husbandry practices.</p>","PeriodicalId":19657,"journal":{"name":"Oxidative Medicine and Cellular Longevity","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11321896/pdf/","citationCount":"0","resultStr":"{\"title\":\"Quercetin Increases Growth Performance and Decreases Incidence of Diarrhea and Mechanism of Action in Weaned Piglets.\",\"authors\":\"Yanjun Mao, Qinglin Yang, Junhong Liu, Yuxin Fu, Shuaishuai Zhou, Jiayan Liu, Linlin Ying, Yao Li\",\"doi\":\"10.1155/2024/5632260\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>This study aimed to investigate the mechanism of quercetin increasing growth performance and decreasing incidence of diarrhea in weaned piglets. Forty-eight Duroc × Landrace × Large White weaned piglets with similar body weight (7.48 ± 0.20 kg, 28 days of age) were randomly divided into four treatments (control, 250 mg/kg quercetin, 500 mg/kg quercetin, and 750 mg/kg quercetin treatments) and fed with basal diet or experimental diet supplemented with quercetin. Performance, diarrhea rate and index, and content of serum anti-inflammatory factors were determined and calculated in weaned piglets; colonic flora and signaling pathways related to anti-inflammation were measured using 16S rDNA sequencing and RNA-seq, respectively. The results showed that compared with control, feed-to-gain ratio and content of serum interferon gamma (IFN-<i>γ</i>) were significantly decreased in the 500 and 750 mg/kg quercetin treatments (<i>P</i> < 0.05); quercetin significantly decreased diarrhea rate and diarrhea index (<i>P</i> < 0.05) and significantly increased the content of serum transforming growth factor (TGF-<i>β</i>) in weaned piglets (<i>P</i> < 0.05); the content of serum NF-<i>κ</i>B was significantly decreased in the 750 mg/kg quercetin treatment (<i>P</i> < 0.05); moreover, quercetin significantly increased diversity of colonic flora (<i>P</i> < 0.05), and at the phylum level, the relative abundance of Actinobacteria in the 500 and 750 mg/kg treatments was significantly increased (<i>P</i> < 0.05), and the relative abundance of Proteobacteria in the three quercetin treatments were significantly decreased (<i>P</i> < 0.05) in the colon of weaned piglets; at the genus level, the relative abundance of <i>Clostridium-sensu-stricto-1</i>, <i>Turicibacter</i>, <i>unclassified_f_Lachnospiraceae</i>, <i>Phascolarctobacterium</i>, and <i>Family_XIII _AD3011_group</i> was significantly increased (<i>P</i> < 0.05); the relative abundance of <i>Subdollgranulum</i> and <i>Blautia</i> was significantly decreased in the 500 and 750 mg/kg treatments (<i>P</i> < 0.05); the relative abundance of <i>Eschericha-Shigella</i>, <i>Terrisporobacter</i>, and <i>Eubacterium-coprostanoligenes</i> was significantly increased (<i>P</i> < 0.05); the relative abundance of <i>Streptocococcus</i>, <i>Sarcina</i>, <i>Staphylococcus</i>, and <i>Ruminococcaceae_UCG-008</i> was significantly decreased in the three quercetin treatments (<i>P</i> < 0.05); the relative abundance of <i>Ruminococcaceae_UCG_014</i> was significantly increased in the 250 mg/kg quercetin treatment in the colon of weaned piglets (<i>P</i> < 0.05). The results of Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that differentially expressed genes (DEGs) from the quercetin treatments were significantly enriched in nuclear transcription factor-<i>κ</i>B (NF-<i>κ</i>B) signal pathway (<i>P</i> < 0.05); mRNA expression of tumor necrosis factor-<i>α</i> (TNF-<i>α</i>), interleukin-1R1 (IL-1R1), conserved helix-loop-helix ubiquitous kinase (CHUK), toll-like receptor 4 (TLR4), and IL-1<i>β</i> from quercetin treatments were significantly decreased in colonic mucosa of weaned piglets (<i>P</i> < 0.05). In summary, quercetin increased feed conversion ratio and decreased diarrhea through regulating NF-<i>κ</i>B signaling pathway, controlling the balance between anti-inflammatory and proinflammatory factors, and modulating intestinal flora, thus promoting the absorption of nutrients in weaned piglets. These results provided the theoretical foundation for applying quercetin in preventing weaning piglets' diarrhea and animal husbandry practices.</p>\",\"PeriodicalId\":19657,\"journal\":{\"name\":\"Oxidative Medicine and Cellular Longevity\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-08-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11321896/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Oxidative Medicine and Cellular Longevity\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1155/2024/5632260\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q1\",\"JCRName\":\"Biochemistry, Genetics and Molecular Biology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Oxidative Medicine and Cellular Longevity","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1155/2024/5632260","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
Quercetin Increases Growth Performance and Decreases Incidence of Diarrhea and Mechanism of Action in Weaned Piglets.
This study aimed to investigate the mechanism of quercetin increasing growth performance and decreasing incidence of diarrhea in weaned piglets. Forty-eight Duroc × Landrace × Large White weaned piglets with similar body weight (7.48 ± 0.20 kg, 28 days of age) were randomly divided into four treatments (control, 250 mg/kg quercetin, 500 mg/kg quercetin, and 750 mg/kg quercetin treatments) and fed with basal diet or experimental diet supplemented with quercetin. Performance, diarrhea rate and index, and content of serum anti-inflammatory factors were determined and calculated in weaned piglets; colonic flora and signaling pathways related to anti-inflammation were measured using 16S rDNA sequencing and RNA-seq, respectively. The results showed that compared with control, feed-to-gain ratio and content of serum interferon gamma (IFN-γ) were significantly decreased in the 500 and 750 mg/kg quercetin treatments (P < 0.05); quercetin significantly decreased diarrhea rate and diarrhea index (P < 0.05) and significantly increased the content of serum transforming growth factor (TGF-β) in weaned piglets (P < 0.05); the content of serum NF-κB was significantly decreased in the 750 mg/kg quercetin treatment (P < 0.05); moreover, quercetin significantly increased diversity of colonic flora (P < 0.05), and at the phylum level, the relative abundance of Actinobacteria in the 500 and 750 mg/kg treatments was significantly increased (P < 0.05), and the relative abundance of Proteobacteria in the three quercetin treatments were significantly decreased (P < 0.05) in the colon of weaned piglets; at the genus level, the relative abundance of Clostridium-sensu-stricto-1, Turicibacter, unclassified_f_Lachnospiraceae, Phascolarctobacterium, and Family_XIII _AD3011_group was significantly increased (P < 0.05); the relative abundance of Subdollgranulum and Blautia was significantly decreased in the 500 and 750 mg/kg treatments (P < 0.05); the relative abundance of Eschericha-Shigella, Terrisporobacter, and Eubacterium-coprostanoligenes was significantly increased (P < 0.05); the relative abundance of Streptocococcus, Sarcina, Staphylococcus, and Ruminococcaceae_UCG-008 was significantly decreased in the three quercetin treatments (P < 0.05); the relative abundance of Ruminococcaceae_UCG_014 was significantly increased in the 250 mg/kg quercetin treatment in the colon of weaned piglets (P < 0.05). The results of Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that differentially expressed genes (DEGs) from the quercetin treatments were significantly enriched in nuclear transcription factor-κB (NF-κB) signal pathway (P < 0.05); mRNA expression of tumor necrosis factor-α (TNF-α), interleukin-1R1 (IL-1R1), conserved helix-loop-helix ubiquitous kinase (CHUK), toll-like receptor 4 (TLR4), and IL-1β from quercetin treatments were significantly decreased in colonic mucosa of weaned piglets (P < 0.05). In summary, quercetin increased feed conversion ratio and decreased diarrhea through regulating NF-κB signaling pathway, controlling the balance between anti-inflammatory and proinflammatory factors, and modulating intestinal flora, thus promoting the absorption of nutrients in weaned piglets. These results provided the theoretical foundation for applying quercetin in preventing weaning piglets' diarrhea and animal husbandry practices.
期刊介绍:
Oxidative Medicine and Cellular Longevity is a unique peer-reviewed, Open Access journal that publishes original research and review articles dealing with the cellular and molecular mechanisms of oxidative stress in the nervous system and related organ systems in relation to aging, immune function, vascular biology, metabolism, cellular survival and cellular longevity. Oxidative stress impacts almost all acute and chronic progressive disorders and on a cellular basis is intimately linked to aging, cardiovascular disease, cancer, immune function, metabolism and neurodegeneration. The journal fills a significant void in today’s scientific literature and serves as an international forum for the scientific community worldwide to translate pioneering “bench to bedside” research into clinical strategies.