通过 LC-MS/MS 对抗凝剂治疗与人体内维生素 K 同源物之间的联系进行研究。

IF 1.9 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS

Bioanalysis Pub Date : 2024-08-14 DOI:10.1080/17576180.2024.2383109

Xiemin Qi, Ning Chen, Qinxin Song, Yanan Chu, Haiping Wu, Jingwen Shan, Huijie Yue, Guohua Zhou

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引用次数: 0

摘要

目的：各种维生素 K（VK）的生物活性存在差异。为了研究初始抗凝的临床参数与血浆中 VK1 和 VK2（MK-4 和 MK-7）水平之间的相关性，有必要建立一种同时测定的定量方法：用环己烷提取心血管病人的血浆样本，然后用 C18 色谱柱进行分析。VK1、MK-4 和 MK-7 的基线浓度分别为 0.98 ± 0.52 纳克/毫升、0.45 ± 0.13 纳克/毫升和 0.65 ± 0.31 纳克/毫升。MK-7和总VK的浓度分别与INR0显著相关（分别为p = 0.010和p = 0.048）：因此，在调整抗凝剂量时，可考虑各种 VK 同源物的浓度。

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The investigation of connection between anticoagulant therapy and vitamin K homologues in human determined by LC-MS/MS.

Aim: Differences are existed in the bioactivity among various vitamin K (VK) forms. To investigate the correlation between clinical parameters of initial anticoagulation and plasma levels of VK1 and VK2 (MK-4 and MK-7), it was necessary to establish a quantitative method for simultaneous determination.Materials & methods: Plasma samples in cardiovascular patients were extracted by cyclohexane and analyzed using a C18 column. Baseline concentrations of VK1, MK-4 and MK-7 were 0.98 ± 0.52 ng/ml, 0.45 ± 0.13 ng/ml and 0.65 ± 0.31 ng/ml, respectively. The concentrations of MK-7 and total VKs were significantly relevant to INR0, respectively (p = 0.010 and p = 0.048, respectively).Conclusion: Thus, when adjusting anticoagulation dosage, concentrations of various VK homologues might be considered.

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来源期刊

Bioanalysis BIOCHEMICAL RESEARCH METHODS-CHEMISTRY, ANALYTICAL

CiteScore

3.30

自引率

16.70%

发文量

审稿时长

2 months

期刊介绍： Reliable data obtained from selective, sensitive and reproducible analysis of xenobiotics and biotics in biological samples is a fundamental and crucial part of every successful drug development program. The same principles can also apply to many other areas of research such as forensic science, toxicology and sports doping testing. The bioanalytical field incorporates sophisticated techniques linking sample preparation and advanced separations with MS and NMR detection systems, automation and robotics. Standards set by regulatory bodies regarding method development and validation increasingly define the boundaries between speed and quality. Bioanalysis is a progressive discipline for which the future holds many exciting opportunities to further reduce sample volumes, analysis cost and environmental impact, as well as to improve sensitivity, specificity, accuracy, efficiency, assay throughput, data quality, data handling and processing. The journal Bioanalysis focuses on the techniques and methods used for the detection or quantitative study of analytes in human or animal biological samples. Bioanalysis encourages the submission of articles describing forward-looking applications, including biosensors, microfluidics, miniaturized analytical devices, and new hyphenated and multi-dimensional techniques. Bioanalysis delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for the modern bioanalyst.