用 ARTP 诱变方法诱变 Aureobasidium pullulans RM1603，使其产生高产的纤维素，并对突变体进行转录组分析。

IF 2.3 3区生物学 Q3 MICROBIOLOGY

Archives of Microbiology Pub Date : 2024-08-14 DOI:10.1007/s00203-024-04094-1

Ruoxuan Bai, Jiale Chen, Yaqiao Hao, Yiheng Dong, Keyao Ren, Ting Gao, Shuting Zhang, Fangxu Xu, Hongxin Zhao

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引用次数: 0

摘要

普鲁兰是由金杆菌（Aureobasidium spp.）产生的一种微生物外多糖，具有优异的物理和化学性质，因而具有极大的应用价值。本研究对从根瘤土壤中分离到的一株高产普鲁兰（51.0 ± 1.0 g-L- 1）的新型普鲁兰Aureobasidium菌株RM1603进行了大气和室温等离子体（ARTP）诱变，然后选择突变体以获得普鲁兰高产菌株。最终，获得了两个突变体 Mu0816 和 Mu1519，发酵 72 小时后多糖产量分别为 58.7 ± 0.8 和 60.0 ± 0.8 g∙L- 1，与原始菌株相比分别增加了 15.1% 和 17.6%。对两个突变体和原始菌株的转录组分析表明，突变体中的α/β-水解酶（ABHD）、α-淀粉酶（AMY1）和糖搬运工家族MFS转运体（SPF-MFS）的高表达可能与葡聚糖的合成和分泌有关。这些结果表明，ARTP诱变技术在A. pullulans中是有效的，为研究与pullulan合成和分泌有关的基因提供了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

ARTP mutagenesis of Aureobasidium pullulans RM1603 for high pullulan production and transcriptome analysis of mutants

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ARTP mutagenesis of Aureobasidium pullulans RM1603 for high pullulan production and transcriptome analysis of mutants

Pullulan is a microbial exopolysaccharide produced by Aureobasidium spp. with excellent physical and chemical properties, resulting in great application value. In this study, a novel strain RM1603 of Aureobasidium pullulans with high pullulan production of 51.0 ± 1.0 g·L^− 1 isolated from rhizosphere soil was subjected to atmospheric and room temperature plasma (ARTP) mutagenesis, followed by selection of mutants to obtain pullulan high-producing strains. Finally, two mutants Mu0816 and Mu1519 were obtained, with polysaccharide productions of 58.7 ± 0.8 and 60.0 ± 0.8 g∙L^− 1 after 72-h fermentation, representing 15.1 and 17.6% increases compared with the original strain, respectively. Transcriptome analysis of the two mutants and the original strain revealed that the high expression of α/β-hydrolase (ABHD), α-amylase (AMY1), and sugar porter family MFS transporters (SPF-MFS) in the mutants may be related to the synthesis and secretion of pullulan. These results demonstrated the effectiveness of ARTP mutagenesis in A. pullulans, providing a basis for the investigation of genes related to pullulan synthesis and secretion.

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来源期刊

Archives of Microbiology 生物-微生物学

CiteScore

4.90

自引率

3.60%

发文量

601

审稿时长

3 months

期刊介绍： Research papers must make a significant and original contribution to microbiology and be of interest to a broad readership. The results of any experimental approach that meets these objectives are welcome, particularly biochemical, molecular genetic, physiological, and/or physical investigations into microbial cells and their interactions with their environments, including their eukaryotic hosts. Mini-reviews in areas of special topical interest and papers on medical microbiology, ecology and systematics, including description of novel taxa, are also published. Theoretical papers and those that report on the analysis or ''mining'' of data are acceptable in principle if new information, interpretations, or hypotheses emerge.