在光谱紧凑型 CE 系统上验证 PowerPlex®35GY 系统:新型八染 STR 多路试剂盒。

IF 2.2 3区 医学 Q1 MEDICINE, LEGAL
International Journal of Legal Medicine Pub Date : 2024-11-01 Epub Date: 2024-08-13 DOI:10.1007/s00414-024-03308-x
Weifeng Qu, Jinjie Liu, Lei Guo, Feng Wang, Zheng Gong, Yanan Liu, Yi Liu, Hongtao Jia, Haibo Rong, Mao Li, Penghua Wei, Dan Wen, Chudong Wang, Ruyi Xu, Xuan Tang, Siqi Chen, Xiaoyi Fu, Xue Li, Yue Wang, Yuepeng Wang, Tao Zhang, Yuguang Wang, Li Chen, Jienan Li, Ying Liu, Jifeng Cai, Bowei Jiang, Lagabaiyila Zha
{"title":"在光谱紧凑型 CE 系统上验证 PowerPlex®35GY 系统:新型八染 STR 多路试剂盒。","authors":"Weifeng Qu, Jinjie Liu, Lei Guo, Feng Wang, Zheng Gong, Yanan Liu, Yi Liu, Hongtao Jia, Haibo Rong, Mao Li, Penghua Wei, Dan Wen, Chudong Wang, Ruyi Xu, Xuan Tang, Siqi Chen, Xiaoyi Fu, Xue Li, Yue Wang, Yuepeng Wang, Tao Zhang, Yuguang Wang, Li Chen, Jienan Li, Ying Liu, Jifeng Cai, Bowei Jiang, Lagabaiyila Zha","doi":"10.1007/s00414-024-03308-x","DOIUrl":null,"url":null,"abstract":"<p><p>The PowerPlex<sup>®</sup> 35GY System (Promega, USA) is an advanced eight-dye multiplex STR kit, incorporating twenty-three autosomal STR loci, eleven Y chromosome STR loci, one sex determining marker Amelogenin, and two quality indicators. This multiplex system includes 20 CODIS loci and up to 15 mini-STR loci with sizing values less than 250 bases. In this study, validation for PowerPlex<sup>®</sup> 35GY System was conducted following the guidelines of SWGDAM, encompassing sensitivity, precision, accuracy, concordance, species specificity, stutter, mixture, stability, and degraded DNA. The results from experiments demonstrated that the PowerPlex<sup>®</sup> 35GY System could effectively amplify DNA samples, with complete allele detection achieved at 125 pg. Moreover, over 90% of alleles from minor contributors were detected at a mixed ratio of 1:4. Additionally, the system was found to yield full profiles even in the presence of hematin, humic acid, and indigo. The PowerPlex<sup>®</sup> 35GY System demonstrated superior performance in the sensitivity and degraded DNA studies compared to a six-dye STR kit. Hence, it is evident that the PowerPlex<sup>®</sup> 35GY System is well-suited for forensic practice, whether in casework or for database samples. These findings provide strong support for the efficacy and reliability of the PowerPlex<sup>®</sup> 35GY System in forensic applications.</p>","PeriodicalId":14071,"journal":{"name":"International Journal of Legal Medicine","volume":" ","pages":"2299-2314"},"PeriodicalIF":2.2000,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Validation of the PowerPlex<sup>®</sup>35GY System: a novel eight-dye STR multiplex kit on the Spectrum Compact CE System.\",\"authors\":\"Weifeng Qu, Jinjie Liu, Lei Guo, Feng Wang, Zheng Gong, Yanan Liu, Yi Liu, Hongtao Jia, Haibo Rong, Mao Li, Penghua Wei, Dan Wen, Chudong Wang, Ruyi Xu, Xuan Tang, Siqi Chen, Xiaoyi Fu, Xue Li, Yue Wang, Yuepeng Wang, Tao Zhang, Yuguang Wang, Li Chen, Jienan Li, Ying Liu, Jifeng Cai, Bowei Jiang, Lagabaiyila Zha\",\"doi\":\"10.1007/s00414-024-03308-x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The PowerPlex<sup>®</sup> 35GY System (Promega, USA) is an advanced eight-dye multiplex STR kit, incorporating twenty-three autosomal STR loci, eleven Y chromosome STR loci, one sex determining marker Amelogenin, and two quality indicators. This multiplex system includes 20 CODIS loci and up to 15 mini-STR loci with sizing values less than 250 bases. In this study, validation for PowerPlex<sup>®</sup> 35GY System was conducted following the guidelines of SWGDAM, encompassing sensitivity, precision, accuracy, concordance, species specificity, stutter, mixture, stability, and degraded DNA. The results from experiments demonstrated that the PowerPlex<sup>®</sup> 35GY System could effectively amplify DNA samples, with complete allele detection achieved at 125 pg. Moreover, over 90% of alleles from minor contributors were detected at a mixed ratio of 1:4. Additionally, the system was found to yield full profiles even in the presence of hematin, humic acid, and indigo. The PowerPlex<sup>®</sup> 35GY System demonstrated superior performance in the sensitivity and degraded DNA studies compared to a six-dye STR kit. Hence, it is evident that the PowerPlex<sup>®</sup> 35GY System is well-suited for forensic practice, whether in casework or for database samples. These findings provide strong support for the efficacy and reliability of the PowerPlex<sup>®</sup> 35GY System in forensic applications.</p>\",\"PeriodicalId\":14071,\"journal\":{\"name\":\"International Journal of Legal Medicine\",\"volume\":\" \",\"pages\":\"2299-2314\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2024-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Legal Medicine\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1007/s00414-024-03308-x\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/8/13 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"MEDICINE, LEGAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Legal Medicine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s00414-024-03308-x","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/8/13 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"MEDICINE, LEGAL","Score":null,"Total":0}
引用次数: 0

摘要

PowerPlex® 35GY 系统(美国 Promega 公司)是一种先进的八染多重 STR 试剂盒,包含 23 个常染色体 STR 位点、11 个 Y 染色体 STR 位点、一个性别决定标记 Amelogenin 和两个质量指标。该多重系统包括 20 个 CODIS 位点和多达 15 个大小值小于 250 碱基的迷你 STR 位点。本研究按照 SWGDAM 的指导方针对 PowerPlex® 35GY 系统进行了验证,包括灵敏度、精确度、准确性、一致性、物种特异性、滞后、混合、稳定性和降解 DNA。实验结果表明,PowerPlex® 35GY 系统能有效扩增 DNA 样品,在 125 pg 时就能检测到完整的等位基因。此外,该系统还能在血红素、腐植酸和靛蓝存在的情况下生成完整的图谱。与六染 STR 试剂盒相比,PowerPlex® 35GY 系统在灵敏度和降解 DNA 研究中表现出更优越的性能。因此,PowerPlex® 35GY 系统显然非常适合法医实践,无论是在案件工作中还是在数据库样本中。这些研究结果有力地证明了 PowerPlex® 35GY 系统在法医应用中的有效性和可靠性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Validation of the PowerPlex<sup>®</sup>35GY System: a novel eight-dye STR multiplex kit on the Spectrum Compact CE System.

Validation of the PowerPlex®35GY System: a novel eight-dye STR multiplex kit on the Spectrum Compact CE System.

The PowerPlex® 35GY System (Promega, USA) is an advanced eight-dye multiplex STR kit, incorporating twenty-three autosomal STR loci, eleven Y chromosome STR loci, one sex determining marker Amelogenin, and two quality indicators. This multiplex system includes 20 CODIS loci and up to 15 mini-STR loci with sizing values less than 250 bases. In this study, validation for PowerPlex® 35GY System was conducted following the guidelines of SWGDAM, encompassing sensitivity, precision, accuracy, concordance, species specificity, stutter, mixture, stability, and degraded DNA. The results from experiments demonstrated that the PowerPlex® 35GY System could effectively amplify DNA samples, with complete allele detection achieved at 125 pg. Moreover, over 90% of alleles from minor contributors were detected at a mixed ratio of 1:4. Additionally, the system was found to yield full profiles even in the presence of hematin, humic acid, and indigo. The PowerPlex® 35GY System demonstrated superior performance in the sensitivity and degraded DNA studies compared to a six-dye STR kit. Hence, it is evident that the PowerPlex® 35GY System is well-suited for forensic practice, whether in casework or for database samples. These findings provide strong support for the efficacy and reliability of the PowerPlex® 35GY System in forensic applications.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
5.80
自引率
9.50%
发文量
165
审稿时长
1 months
期刊介绍: The International Journal of Legal Medicine aims to improve the scientific resources used in the elucidation of crime and related forensic applications at a high level of evidential proof. The journal offers review articles tracing development in specific areas, with up-to-date analysis; original articles discussing significant recent research results; case reports describing interesting and exceptional examples; population data; letters to the editors; and technical notes, which appear in a section originally created for rapid publication of data in the dynamic field of DNA analysis.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信