{"title":"LncRNA-Snhg3调控正常进食条件下的小鼠肝糖生成","authors":"Xianghong Xie, Mingyue Gao, Heping Wang, Minglong Zhang, Wei Zhao, Chunmei Li, Weihong Zhang, Jiahui Yang, Yinliang Zhang, Enhui Chen, Yanfang Guo, Zeyu Guo, Ebenezeri Erasto Ngowi, Xiaoman Wang, Yinghan Zhu, Yiting Wang, Xiaolu Li, Hong Yao, Li Yan, Fude Fang, Meixia Li, Aijun Qiao, Xiaojun Liu","doi":"10.1096/fj.202401064R","DOIUrl":null,"url":null,"abstract":"<p>Long noncoding RNAs (lncRNAs) are strongly associated with glucose homeostasis, but their roles remain largely unknown. In this study, the potential role of lncRNA-<i>Snhg3</i> in glucose metabolism was evaluated both in vitro and in vivo. Here, we found a positive relationship between <i>Snhg3</i> and hepatic glycogenesis. Glucose tolerance improved in hepatocyte-specific <i>Snhg3</i> knock-in (<i>Snhg3</i>-HKI) mice, while it worsened in hepatocyte-specific <i>Snhg3</i> knockout (<i>Snhg3</i>-HKO) mice. Furthermore, hepatic glycogenesis had shown remarkable increase in <i>Snhg3</i>-HKI mice and reduction in <i>Snhg3</i>-HKO mice, respectively. Mechanistically, <i>Snhg3</i> increased mRNA and protein expression levels of PPP1R3B through inducing chromatin remodeling and promoting the phosphorylation of protein kinase B. Collectively, these results suggested that lncRNA-<i>Snhg3</i> plays a critical role in hepatic glycogenesis.</p>","PeriodicalId":50455,"journal":{"name":"FASEB Journal","volume":null,"pages":null},"PeriodicalIF":4.4000,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"LncRNA-Snhg3 regulates mouse hepatic glycogenesis under normal chow diet\",\"authors\":\"Xianghong Xie, Mingyue Gao, Heping Wang, Minglong Zhang, Wei Zhao, Chunmei Li, Weihong Zhang, Jiahui Yang, Yinliang Zhang, Enhui Chen, Yanfang Guo, Zeyu Guo, Ebenezeri Erasto Ngowi, Xiaoman Wang, Yinghan Zhu, Yiting Wang, Xiaolu Li, Hong Yao, Li Yan, Fude Fang, Meixia Li, Aijun Qiao, Xiaojun Liu\",\"doi\":\"10.1096/fj.202401064R\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Long noncoding RNAs (lncRNAs) are strongly associated with glucose homeostasis, but their roles remain largely unknown. In this study, the potential role of lncRNA-<i>Snhg3</i> in glucose metabolism was evaluated both in vitro and in vivo. Here, we found a positive relationship between <i>Snhg3</i> and hepatic glycogenesis. Glucose tolerance improved in hepatocyte-specific <i>Snhg3</i> knock-in (<i>Snhg3</i>-HKI) mice, while it worsened in hepatocyte-specific <i>Snhg3</i> knockout (<i>Snhg3</i>-HKO) mice. Furthermore, hepatic glycogenesis had shown remarkable increase in <i>Snhg3</i>-HKI mice and reduction in <i>Snhg3</i>-HKO mice, respectively. Mechanistically, <i>Snhg3</i> increased mRNA and protein expression levels of PPP1R3B through inducing chromatin remodeling and promoting the phosphorylation of protein kinase B. Collectively, these results suggested that lncRNA-<i>Snhg3</i> plays a critical role in hepatic glycogenesis.</p>\",\"PeriodicalId\":50455,\"journal\":{\"name\":\"FASEB Journal\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.4000,\"publicationDate\":\"2024-08-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"FASEB Journal\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1096/fj.202401064R\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"FASEB Journal","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1096/fj.202401064R","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
LncRNA-Snhg3 regulates mouse hepatic glycogenesis under normal chow diet
Long noncoding RNAs (lncRNAs) are strongly associated with glucose homeostasis, but their roles remain largely unknown. In this study, the potential role of lncRNA-Snhg3 in glucose metabolism was evaluated both in vitro and in vivo. Here, we found a positive relationship between Snhg3 and hepatic glycogenesis. Glucose tolerance improved in hepatocyte-specific Snhg3 knock-in (Snhg3-HKI) mice, while it worsened in hepatocyte-specific Snhg3 knockout (Snhg3-HKO) mice. Furthermore, hepatic glycogenesis had shown remarkable increase in Snhg3-HKI mice and reduction in Snhg3-HKO mice, respectively. Mechanistically, Snhg3 increased mRNA and protein expression levels of PPP1R3B through inducing chromatin remodeling and promoting the phosphorylation of protein kinase B. Collectively, these results suggested that lncRNA-Snhg3 plays a critical role in hepatic glycogenesis.
期刊介绍：
The FASEB Journal publishes international, transdisciplinary research covering all fields of biology at every level of organization: atomic, molecular, cell, tissue, organ, organismic and population. While the journal strives to include research that cuts across the biological sciences, it also considers submissions that lie within one field, but may have implications for other fields as well. The journal seeks to publish basic and translational research, but also welcomes reports of pre-clinical and early clinical research. In addition to research, review, and hypothesis submissions, The FASEB Journal also seeks perspectives, commentaries, book reviews, and similar content related to the life sciences in its Up Front section.
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