日本鹌鹑原始生殖细胞的初始形成和性别差异。

IF 2.5 3区生物学 Q3 REPRODUCTIVE BIOLOGY

Reproductive biology Pub Date : 2024-08-09 DOI:10.1016/j.repbio.2024.100922

Shusei Mizushima , Yuya Ogawa , Asato Kuroiwa

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引用次数: 0

摘要

DEAD-box RNA螺旋酶4（DDX4）被认为是调节禽类生殖细胞形成的关键母源生殖细胞因子。我们在本文中利用 CRISPR/Cas9 系统方法结合体外受精技术在日本鹌鹑中发现，与推定原始生殖细胞（PGCs）中细胞周期蛋白 D1 的核定位相关的子代基因组激活的 DDX4 基因产物在原始生殖细胞的增殖过程中起着至关重要的作用。蛋白质组分析还揭示了早期雄性和雌性PGCs的分子特征差异。

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Initial formation of and sex differences in primordial germ cells in Japanese quail

DEAD-box RNA helicase 4 (DDX4) is posited to be a key maternal germ cell factor regulating avian germ cell formation. We herein showed that the DDX4 gene product of zygotic genome activation associated with the nuclear localization of the cyclin D1 protein in presumptive primordial germ cells (PGCs) plays an essential role in the proliferation of PGCs using a CRISPR/Cas9 system approach combined with in vitro fertilization techniques in Japanese quail. A proteome analysis also revealed molecular-based differences in the features of early male and female PGCs.

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来源期刊

Reproductive biology 生物-生殖生物学

CiteScore

3.90

自引率

0.00%

发文量

审稿时长

29 days

期刊介绍： An official journal of the Society for Biology of Reproduction and the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn, Poland. Reproductive Biology is an international, peer-reviewed journal covering all aspects of reproduction in vertebrates. The journal invites original research papers, short communications, review articles and commentaries dealing with reproductive physiology, endocrinology, immunology, molecular and cellular biology, receptor studies, animal breeding as well as andrology, embryology, infertility, assisted reproduction and contraception. Papers from both basic and clinical research will be considered.