Z Rahimi, H Haiarian, H Karamishabankareh, L Soltani
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By using liquid nitrogen vapor, the semen was frozen and stored at 196 degree C.</p><p><strong>Results: </strong>The total motility, viability, and DNA integrity were significantly improved after freeze-thawing with 28 mM inulin, compared to other treatment groups (P < 0.05). A Tris-based extender containing 16 and 28 mM of inulin displayed the highest levels of ram sperm membrane integrity when compared with the control (p <0.05). The abnormality of ram sperm was increased during freeze-thawing at control and 1 mM of inulin, compared to 16 and 28 mM of inulin (P < 0.05). Additionally, 28 mM of inulin decreased MDA and increased SOD activity in ram sperm in comparison with the other treatments (P < 0.05).</p><p><strong>Conclusion: </strong>As a result, 28 mM of inulin could be beneficial for the cryopreservation industry and reduce the harmful effects of freeze-thawing on ram sperm. 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Numerous protective chemicals, including fructans, have been used during sperm cryopreservation.</p><p><strong>Objectives: </strong>To evaluate the effect of different concentrations of the fructosan inulin on ram sperm quality parameters, superoxide dismutase (SOD) activity, and malondialdehyde (MDA) production after freezing and thawing.</p><p><strong>Materials and methods: </strong>The pooled samples from four healthy rams were divided into seven equal aliquots and diluted in a Tris-base extender supplemented with 1, 2, 4, 8, 16, and 28 mM of inulin or without inulin supplementation (control). By using liquid nitrogen vapor, the semen was frozen and stored at 196 degree C.</p><p><strong>Results: </strong>The total motility, viability, and DNA integrity were significantly improved after freeze-thawing with 28 mM inulin, compared to other treatment groups (P < 0.05). 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引用次数: 0
摘要
背景：在生殖生物技术中，精子冷冻保存起着至关重要的作用。冷冻保存精子会产生活性氧（ROS），从而破坏精子的功能和结构能力。在精子冷冻保存过程中使用了许多保护性化学物质，包括果聚糖：评估不同浓度的果聚糖菊粉对冷冻和解冻后公羊精子质量参数、超氧化物歧化酶（SOD）活性和丙二醛（MDA）生成的影响：将四只健康公羊的精子样品分成七份等量的等分样品，在添加了 1、2、4、8、16 和 28 mM 菊粉或未添加菊粉（对照组）的三碱扩增液中稀释。利用液氮蒸汽将精液冷冻并储存在 196 摄氏度的环境中：结果：与其他处理组相比，使用 28 mM 菊粉进行冻融后，精液的总活力、存活率和 DNA 完整性都有明显提高（P < 0.05）。与对照组相比，含有 16 mM 和 28 mM 菊粉的 Tris 基扩展剂显示出最高水平的公羊精子膜完整性（P 结论：28 mM 菊粉对公羊精子膜完整性的影响最大（P < 0.05）：因此，28 毫摩尔菊粉可能对冷冻保存业有益，并能减少冻融对公羊精子的有害影响。Doi.org/10.54680/fr24510110512.
Effect of different concentrations of inulin on ram sperm quality during cryopreservation.
Background: In reproductive biotechnology, sperm cryopreservation has a vital role to play. Cryopreservation of sperm produces reactive oxygen species (ROS), which disrupt sperm function and structural competence. Numerous protective chemicals, including fructans, have been used during sperm cryopreservation.
Objectives: To evaluate the effect of different concentrations of the fructosan inulin on ram sperm quality parameters, superoxide dismutase (SOD) activity, and malondialdehyde (MDA) production after freezing and thawing.
Materials and methods: The pooled samples from four healthy rams were divided into seven equal aliquots and diluted in a Tris-base extender supplemented with 1, 2, 4, 8, 16, and 28 mM of inulin or without inulin supplementation (control). By using liquid nitrogen vapor, the semen was frozen and stored at 196 degree C.
Results: The total motility, viability, and DNA integrity were significantly improved after freeze-thawing with 28 mM inulin, compared to other treatment groups (P < 0.05). A Tris-based extender containing 16 and 28 mM of inulin displayed the highest levels of ram sperm membrane integrity when compared with the control (p <0.05). The abnormality of ram sperm was increased during freeze-thawing at control and 1 mM of inulin, compared to 16 and 28 mM of inulin (P < 0.05). Additionally, 28 mM of inulin decreased MDA and increased SOD activity in ram sperm in comparison with the other treatments (P < 0.05).
Conclusion: As a result, 28 mM of inulin could be beneficial for the cryopreservation industry and reduce the harmful effects of freeze-thawing on ram sperm. Doi.org/10.54680/fr24510110512.
期刊介绍：
A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation
The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.
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