MpR2R3-MYB2 是马钱子油体形成的关键调节因子。

IF 3.6 3区 生物学 Q1 PLANT SCIENCES
Planta Pub Date : 2024-08-09 DOI:10.1007/s00425-024-04498-9
Hiroyoshi Kubo, Kim Sunhwa, Hiroki Teramori, Kojiro Takanashi
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引用次数: 0

摘要

主要结论MpMYB02是行进素积累的调控因子,也是油体形成的关键调控因子。MpMYB02 可诱导 MpSYP12B 的表达,促进油体的形成,进而导致雄黄素的积累。Marchantia polymorpha 中观察到的油体是一个由单位膜包围的细胞器,其中积累了多种次生代谢产物,如马桑亭素和萜烯。我们观察到油体的形成受 MpMYB02 的调控,MpMYB02 是马钱子苷积累的关键调控因子。在 Mpmyb02 突变体中,虽然在 gemma 中出现了特幼细胞,但没有观察到油体。我们将MpMYB02-糖皮质激素受体(GR)(一种类固醇诱导的转录激活剂)引入Mpmyb02,并评估了地塞米松(DEX)对油体形成的影响。在油体发育的最初阶段,我们观察到小球状结构的聚集。DEX处理上调了几个与油体形成有关的基因,包括MpSYP12B。我们的研究结果表明,MpMYB02 不仅在马钱子素积累过程中起着关键作用,而且在油体形成过程中也起着关键作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

MpR2R3-MYB2 is a key regulator of oil body formation in Marchantia polymorpha.

MpR2R3-MYB2 is a key regulator of oil body formation in Marchantia polymorpha.

Main conclusion: MpMYB02, a regulator of marchantin accumulation, also acts as a key regulator of oil body formation. MpMYB02 induces the expression of MpSYP12B and promotes oil body formation, subsequently leading to marchantin accumulation. The oil body observed in Marchantia polymorpha is a cellular organelle surrounded by a unit membrane, accumulating various secondary metabolites such as marchantins and terpenes. We observed that oil body formation is regulated by MpMYB02, a key regulator of marchantin accumulation. In the Mpmyb02 mutant, no oil bodies were observed, although idioblast-like cells were present in the gemma. We introduced MpMYB02-glucocorticoid receptor (GR), a steroid-inducible transcriptional activator, into Mpmyb02 and assessed the effect of dexamethasone (DEX) on oil body formation. Following DEX treatment, transformed liverworts began forming oil bodies within 12 h. During the initial stages of oil body development, we observed the aggregation of small globular structures. DEX treatment upregulated several genes implicated in oil body formation, including MpSYP12B. Our findings underscore that MpMYB02 plays a crucial role not only in marchantin accumulation but also in oil body formation.

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来源期刊
Planta
Planta 生物-植物科学
CiteScore
7.20
自引率
2.30%
发文量
217
审稿时长
2.3 months
期刊介绍: Planta publishes timely and substantial articles on all aspects of plant biology. We welcome original research papers on any plant species. Areas of interest include biochemistry, bioenergy, biotechnology, cell biology, development, ecological and environmental physiology, growth, metabolism, morphogenesis, molecular biology, new methods, physiology, plant-microbe interactions, structural biology, and systems biology.
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