监测果蝇卵子发生过程中的自噬作用

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2024-08-10 DOI:10.1007/7651_2024_563

Mrunmayee Kulkarni, Karan Selarka, Bhupendra V Shravage

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引用次数: 0

摘要

大自噬（以下简称 "自噬"）是一种进化保守的机制，它通过降解溶酶体中的有毒蛋白质和受损细胞器来维持细胞的健康。卵巢等组织由不同类型的细胞组成，而每种细胞类型的自噬程度各不相同。以特定细胞类型的方式研究自噬有助于更好地了解自噬在卵子发生过程中的作用。在这里，我们介绍了利用 Atg8a 和 Ref(2)P 这两种蛋白标记物监测果蝇卵子发生过程中自噬的检测方法。

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Monitoring Autophagy During Drosophila Oogenesis.

Macroautophagy (autophagy hereafter) is an evolutionarily conserved mechanism that maintains the health of cells by degrading toxic proteins and damaged organelles within the lysosomes. Tissues like ovary are made up of heterogeneous cell types and each cell type has distinct levels of autophagy. Studying autophagy in a cell-type specific manner helps better understand the role of autophagy during oogenesis. Here, we describe assays for monitoring autophagy during oogenesis in Drosophila using the two protein markers, Atg8a and Ref(2)P.

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.