埃及伊蚊采血和卵黄发生过程中的酪氨酸转运核糖核酸水平和修饰。

IF 2.3 2区 农林科学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Melissa Kelley, Christopher J Holmes, Cassandra Herbert, Asif Rayhan, Judd Joves, Melissa Uhran, Lucas Klaus, Ronja Frigard, Khwahish Singh, Patrick A Limbach, Balasubrahmanyam Addepalli, Joshua B Benoit
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引用次数: 0

摘要

埃及伊蚊等蚊子必须食用血餐以获得产卵所需的营养。血餐后,转录组和蛋白质组会发生一些变化,这些变化很可能与密码子使用的改变相对应。转运核糖核酸(tRNA)是一种适配器分子,在蛋白质合成过程中读取信使核糖核酸密码子以添加适当的氨基酸。对 tRNA 进行化学修饰可增强密码子解码,提高蛋白质合成的准确性和效率。在这里,我们研究了与埃及蝇血餐和随后的卵黄发生期相关的 tRNA 修饰和转录本。更具体地说,我们评估了血食后关键时期脂肪体中 tRNA 转录本的丰度和修饰水平。根据替代密码子的使用和特定修饰的鉴定,我们发现酪氨酸 tRNA 的转录增加可能是血食后脂肪体中卵黄蛋白合成过程中的关键。总之,tRNA丰度和修饰的变化是蚊子吸血后产生卵黄素过程中的重要因素。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Tyrosine transfer RNA levels and modifications during blood-feeding and vitellogenesis in the mosquito, Aedes aegypti.

Mosquitoes such as Aedes aegypti must consume a blood meal for the nutrients necessary for egg production. Several transcriptome and proteome changes occur post-blood meal that likely corresponds with codon usage alterations. Transfer RNA (tRNA) is the adapter molecule that reads messenger RNA codons to add the appropriate amino acid during protein synthesis. Chemical modifications to tRNA enhance codon decoding, improving the accuracy and efficiency of protein synthesis. Here, we examined tRNA modifications and transcripts associated with the blood meal and subsequent periods of vitellogenesis in A. aegypti. More specifically, we assessed tRNA transcript abundance and modification levels in the fat body at critical times post blood-feeding. Based on a combination of alternative codon usage and identification of particular modifications, we discovered that increased transcription of tyrosine tRNAs is likely critical during the synthesis of egg yolk proteins in the fat body following a blood meal. Altogether, changes in both the abundance and modification of tRNA are essential factors in the process of vitellogenin production after blood-feeding in mosquitoes.

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来源期刊
Insect Molecular Biology
Insect Molecular Biology 生物-昆虫学
CiteScore
4.80
自引率
3.80%
发文量
68
审稿时长
6-12 weeks
期刊介绍: Insect Molecular Biology has been dedicated to providing researchers with the opportunity to publish high quality original research on topics broadly related to insect molecular biology since 1992. IMB is particularly interested in publishing research in insect genomics/genes and proteomics/proteins. This includes research related to: • insect gene structure • control of gene expression • localisation and function/activity of proteins • interactions of proteins and ligands/substrates • effect of mutations on gene/protein function • evolution of insect genes/genomes, especially where principles relevant to insects in general are established • molecular population genetics where data are used to identify genes (or regions of genomes) involved in specific adaptations • gene mapping using molecular tools • molecular interactions of insects with microorganisms including Wolbachia, symbionts and viruses or other pathogens transmitted by insects Papers can include large data sets e.g.from micro-array or proteomic experiments or analyses of genome sequences done in silico (subject to the data being placed in the context of hypothesis testing).
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