{"title":"转录因子 ABI5 对拟南芥生长和发育的影响","authors":"Xin Chen, Changze Han, Rongrong Yang, Xinwen Wang, Jianzhong Ma, Yonggang Wang","doi":"10.1016/j.jplph.2024.154316","DOIUrl":null,"url":null,"abstract":"<div><p>ABA-insensitive 5 (ABI5) belongs to the basic leucine zipper class of transcription factors and is named for being the fifth identified <em>Arabidopsis</em> mutant unresponsive to ABA. To understand the influence of ABI5 in its active state on downstream gene expression and plant growth and development, we overexpressed the full-length ABI5 (<em>A.t.</em>MX-4) and the active forms of ABI5 with deleted transcriptional repression domains (<em>A.t.</em>MX-1, <em>A.t.</em>MX-2, and <em>A.t.</em>MX-3). Compared with the wild type, <em>A.t.</em>MX-1, <em>A.t.</em>MX-2, and <em>A.t.</em>MX-3 exhibited an increase in rosette leaf number and size, earlier flowering, increased thousand-seed weight, and significantly enhanced drought resistance. Thirty-five upregulated/downregulated proteins in the <em>A.t.</em>MX-1 were identified by proteomic analysis, and these proteins were involved in ABA biosynthesis and degradation, abiotic stress, fatty acid synthesis, and energy metabolism. These proteins participate in the regulation of plant drought resistance, flowering timing, and seed size at the levels of transcription and post-translational modification.</p></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"302 ","pages":"Article 154316"},"PeriodicalIF":4.0000,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Influence of the transcription factor ABI5 on growth and development in Arabidopsis\",\"authors\":\"Xin Chen, Changze Han, Rongrong Yang, Xinwen Wang, Jianzhong Ma, Yonggang Wang\",\"doi\":\"10.1016/j.jplph.2024.154316\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>ABA-insensitive 5 (ABI5) belongs to the basic leucine zipper class of transcription factors and is named for being the fifth identified <em>Arabidopsis</em> mutant unresponsive to ABA. To understand the influence of ABI5 in its active state on downstream gene expression and plant growth and development, we overexpressed the full-length ABI5 (<em>A.t.</em>MX-4) and the active forms of ABI5 with deleted transcriptional repression domains (<em>A.t.</em>MX-1, <em>A.t.</em>MX-2, and <em>A.t.</em>MX-3). Compared with the wild type, <em>A.t.</em>MX-1, <em>A.t.</em>MX-2, and <em>A.t.</em>MX-3 exhibited an increase in rosette leaf number and size, earlier flowering, increased thousand-seed weight, and significantly enhanced drought resistance. Thirty-five upregulated/downregulated proteins in the <em>A.t.</em>MX-1 were identified by proteomic analysis, and these proteins were involved in ABA biosynthesis and degradation, abiotic stress, fatty acid synthesis, and energy metabolism. These proteins participate in the regulation of plant drought resistance, flowering timing, and seed size at the levels of transcription and post-translational modification.</p></div>\",\"PeriodicalId\":16808,\"journal\":{\"name\":\"Journal of plant physiology\",\"volume\":\"302 \",\"pages\":\"Article 154316\"},\"PeriodicalIF\":4.0000,\"publicationDate\":\"2024-07-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of plant physiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0176161724001470\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of plant physiology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0176161724001470","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
摘要
拟南芥对 ABA 不敏感的 5（ABI5）属于碱性亮氨酸拉链类转录因子，因其是拟南芥中第五个确定的对 ABA 不敏感的突变体而得名。为了了解活性状态下的 ABI5 对下游基因表达和植物生长发育的影响，我们过表达了全长 ABI5（A.t.MX-4）和删除了转录抑制结构域的 ABI5 活性形式（A.t.MX-1、A.t.MX-2 和 A.t.MX-3）。与野生型相比，A.t.MX-1、A.t.MX-2 和 A.t.MX-3 表现出莲座叶数量和大小增加、开花提前、千粒重增加以及抗旱性显著增强。通过蛋白质组分析发现，A.t.MX-1 中有 35 个上调/下调的蛋白质，这些蛋白质参与了 ABA 的生物合成和降解、非生物胁迫、脂肪酸合成和能量代谢。这些蛋白质在转录和翻译后修饰水平上参与植物抗旱性、开花时间和种子大小的调控。
Influence of the transcription factor ABI5 on growth and development in Arabidopsis
ABA-insensitive 5 (ABI5) belongs to the basic leucine zipper class of transcription factors and is named for being the fifth identified Arabidopsis mutant unresponsive to ABA. To understand the influence of ABI5 in its active state on downstream gene expression and plant growth and development, we overexpressed the full-length ABI5 (A.t.MX-4) and the active forms of ABI5 with deleted transcriptional repression domains (A.t.MX-1, A.t.MX-2, and A.t.MX-3). Compared with the wild type, A.t.MX-1, A.t.MX-2, and A.t.MX-3 exhibited an increase in rosette leaf number and size, earlier flowering, increased thousand-seed weight, and significantly enhanced drought resistance. Thirty-five upregulated/downregulated proteins in the A.t.MX-1 were identified by proteomic analysis, and these proteins were involved in ABA biosynthesis and degradation, abiotic stress, fatty acid synthesis, and energy metabolism. These proteins participate in the regulation of plant drought resistance, flowering timing, and seed size at the levels of transcription and post-translational modification.
期刊介绍：
The Journal of Plant Physiology is a broad-spectrum journal that welcomes high-quality submissions in all major areas of plant physiology, including plant biochemistry, functional biotechnology, computational and synthetic plant biology, growth and development, photosynthesis and respiration, transport and translocation, plant-microbe interactions, biotic and abiotic stress. Studies are welcome at all levels of integration ranging from molecules and cells to organisms and their environments and are expected to use state-of-the-art methodologies. Pure gene expression studies are not within the focus of our journal. To be considered for publication, papers must significantly contribute to the mechanistic understanding of physiological processes, and not be merely descriptive, or confirmatory of previous results. We encourage the submission of papers that explore the physiology of non-model as well as accepted model species and those that bridge basic and applied research. For instance, studies on agricultural plants that show new physiological mechanisms to improve agricultural efficiency are welcome. Studies performed under uncontrolled situations (e.g. field conditions) not providing mechanistic insight will not be considered for publication.
The Journal of Plant Physiology publishes several types of articles: Original Research Articles, Reviews, Perspectives Articles, and Short Communications. Reviews and Perspectives will be solicited by the Editors; unsolicited reviews are also welcome but only from authors with a strong track record in the field of the review. Original research papers comprise the majority of published contributions.
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