流式细胞仪共享资源实验室用户咨询最佳实践。

IF 2.5 4区 生物学 Q3 BIOCHEMICAL RESEARCH METHODS
Kewal Asosingh, Alice Bayiyana, Michele C. Black, Uttara Chakraborty, Michael J. Clemente, Amy C. Graham, Michael D. Gregory, Karen G. Hogg, Gert Van Isterdael, ChunChun Liu, Lola Martínez, Charlotte C. Petersen, Ziv Porat, Kylie M. Price, Laura B. Prickett, Aja M. Rieger, Caroline E. Roe, Erica Smit
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引用次数: 0

摘要

这篇题为 "用户咨询的最佳实践 "的文章是 2022 年国际流式细胞仪促进会(ISAC)会员调查的结果,该调查收集了共享研究实验室(SRL)社区的宝贵意见,也是 2022 年 CYTO 同名研讨会小组讨论的结果。其中一个重要启示是在流式细胞仪项目开始时启动咨询的重要性,尤其是对实习生而言。这种方法可以改进从规划实验到解释数据的每一个步骤,并使之标准化。这种积极主动的方法能有效减少实验偏差,避免多余的试验和错误,从而节省宝贵的时间和资源。除指导方针外,用户咨询的最佳方法还规定了沟通渠道、方法和关键信息,从而建立了 SRL 与用户之间富有成效的通信结构。该框架是建立稳健、自主的合作关系的典范。用户咨询通过为研究人员提供必要的信息,帮助他们进行符合科学严谨性的可重现流式细胞仪实验,从而实现增值。通过遵循这些最佳实践中概述的步骤、说明和策略,SRL 可以根据自身情况对其进行调整,建立个性化的工作流程,使用户咨询服务正规化。这篇文章为提高流式细胞仪研究的质量和效率提供了实用指南,并汇集了流式细胞仪自学实验室社区在用户咨询方面的集体知识。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Best practices for user consultation in flow cytometry shared resource laboratories

This “Best Practices in User Consultation” article is the result of a 2022 International Society for the Advancement of Cytometry (ISAC) membership survey that collected valuable insights from the shared research laboratory (SRL) community and of a group discussion at the CYTO 2022 workshop of the same name. One key takeaway is the importance of initiating a consultation at the outset of a flow cytometry project, particularly for trainees. This approach enables the improvement and standardization of every step, from planning experiments to interpreting data. This proactive approach effectively mitigates experimental bias and avoids superfluous trial and error, thereby conserving valuable time and resources. In addition to guidelines, the optimal approaches for user consultation specify communication channels, methods, and critical information, thereby establishing a structure for productive correspondence between SRL and users. This framework functions as an exemplar for establishing robust and autonomous collaborative relationships. User consultation adds value by providing researchers with the necessary information to conduct reproducible flow cytometry experiments that adhere to scientific rigor. By following the steps, instructions, and strategies outlined in these best practices, an SRL can readily tailor them to its own setting, establishing a personalized workflow and formalizing user consultation services. This article provides a pragmatic guide for improving the caliber and efficacy of flow cytometry research and aggregates the flow cytometry SRL community's collective knowledge regarding user consultation.

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来源期刊
Cytometry Part A
Cytometry Part A 生物-生化研究方法
CiteScore
8.10
自引率
13.50%
发文量
183
审稿时长
4-8 weeks
期刊介绍: Cytometry Part A, the journal of quantitative single-cell analysis, features original research reports and reviews of innovative scientific studies employing quantitative single-cell measurement, separation, manipulation, and modeling techniques, as well as original articles on mechanisms of molecular and cellular functions obtained by cytometry techniques. The journal welcomes submissions from multiple research fields that fully embrace the study of the cytome: Biomedical Instrumentation Engineering Biophotonics Bioinformatics Cell Biology Computational Biology Data Science Immunology Parasitology Microbiology Neuroscience Cancer Stem Cells Tissue Regeneration.
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