基于线粒体 ND4 的双链 PCR 鉴定老虎物种

IF 0.9 4区环境科学与生态学 Q4 BIODIVERSITY CONSERVATION

Conservation Genetics Resources Pub Date : 2024-07-29 DOI:10.1007/s12686-024-01364-4

Yang Gao, Shiyao Wu, Xuanye Wu, Wulin Liu, Dong Zheng, Xuedong Liu, Kun Jin

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引用次数: 0

摘要

为了根据野外调查中收集到的疑似粪便或皮毛样本鉴定老虎的种类，我们开发了一种双工 PCR 系统，该系统可共同扩增两个线粒体 DNA（mtDNA）扩增子，以同时生成细胞色素 b（MT-CYB）基因的 mtDNA 质量标记（221 bp）和 NADH 脱氢酶亚基 4（MT-ND4）基因的老虎标记（314 bp）。前一个标记的出现表明样本中的 mtDNA 质量，而虎标记出现在虎样本中，但不出现在非虎样本中。对疑似老虎粪便样本的盲测验证了我们系统的可靠性，而使用以前发表的方法进行的平行实验进一步证实了我们系统的准确性。这种方法可用于分析可疑样本，因此对老虎的野外管理和保护具有重要的实用价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Mitochondrial ND4-based duplex PCR for identifying tiger species

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Mitochondrial ND4-based duplex PCR for identifying tiger species

To identify tiger species on the basis of suspected feces or fur samples collected during field surveys, we developed a duplex PCR system that co-amplified two mitochondrial DNA (mtDNA) amplicons to simultaneously generate a mtDNA quality marker (221 bp) from the cytochrome b (MT-CYB) gene and a tiger marker (314 bp) from the NADH dehydrogenase subunit 4 (MT-ND4) gene. Appearance of the former marker indicated sample-derived mtDNA quality, and the tiger marker was present in the tiger samples but absent from non-tiger samples. A blind test of a suspected tiger fecal sample validated reliability of our system, and a parallel experiment using a previously published approach further confirmed the accuracy of our system. This method has significant practical value for the field management and conservation of tigers because it can be used to analyze suspected samples.

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来源期刊

Conservation Genetics Resources BIODIVERSITY CONSERVATION-GENETICS & HEREDITY

CiteScore

2.90

自引率

9.10%

发文量

审稿时长

3-6 weeks

期刊介绍： Conservation Genetics Resources promotes the conservation of genetic diversity and advances the study of conservation genetics by providing rapid publication of technical papers and reviews on methodological innovations or improvements, computer programs, and genomic resources, as well as on the practical application of these resources towards the development of effective conservation policy and practice.