Yang Gao, Shiyao Wu, Xuanye Wu, Wulin Liu, Dong Zheng, Xuedong Liu, Kun Jin
{"title":"基于线粒体 ND4 的双链 PCR 鉴定老虎物种","authors":"Yang Gao, Shiyao Wu, Xuanye Wu, Wulin Liu, Dong Zheng, Xuedong Liu, Kun Jin","doi":"10.1007/s12686-024-01364-4","DOIUrl":null,"url":null,"abstract":"<p>To identify tiger species on the basis of suspected feces or fur samples collected during field surveys, we developed a duplex PCR system that co-amplified two mitochondrial DNA (mtDNA) amplicons to simultaneously generate a mtDNA quality marker (221 bp) from the <i>cytochrome b (MT-CYB)</i> gene and a tiger marker (314 bp) from the <i>NADH dehydrogenase subunit 4 (MT-ND4)</i> gene. Appearance of the former marker indicated sample-derived mtDNA quality, and the tiger marker was present in the tiger samples but absent from non-tiger samples. A blind test of a suspected tiger fecal sample validated reliability of our system, and a parallel experiment using a previously published approach further confirmed the accuracy of our system. This method has significant practical value for the field management and conservation of tigers because it can be used to analyze suspected samples.</p>","PeriodicalId":0,"journal":{"name":"","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Mitochondrial ND4-based duplex PCR for identifying tiger species\",\"authors\":\"Yang Gao, Shiyao Wu, Xuanye Wu, Wulin Liu, Dong Zheng, Xuedong Liu, Kun Jin\",\"doi\":\"10.1007/s12686-024-01364-4\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>To identify tiger species on the basis of suspected feces or fur samples collected during field surveys, we developed a duplex PCR system that co-amplified two mitochondrial DNA (mtDNA) amplicons to simultaneously generate a mtDNA quality marker (221 bp) from the <i>cytochrome b (MT-CYB)</i> gene and a tiger marker (314 bp) from the <i>NADH dehydrogenase subunit 4 (MT-ND4)</i> gene. Appearance of the former marker indicated sample-derived mtDNA quality, and the tiger marker was present in the tiger samples but absent from non-tiger samples. A blind test of a suspected tiger fecal sample validated reliability of our system, and a parallel experiment using a previously published approach further confirmed the accuracy of our system. This method has significant practical value for the field management and conservation of tigers because it can be used to analyze suspected samples.</p>\",\"PeriodicalId\":0,\"journal\":{\"name\":\"\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0,\"publicationDate\":\"2024-07-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://doi.org/10.1007/s12686-024-01364-4\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1007/s12686-024-01364-4","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Mitochondrial ND4-based duplex PCR for identifying tiger species
To identify tiger species on the basis of suspected feces or fur samples collected during field surveys, we developed a duplex PCR system that co-amplified two mitochondrial DNA (mtDNA) amplicons to simultaneously generate a mtDNA quality marker (221 bp) from the cytochrome b (MT-CYB) gene and a tiger marker (314 bp) from the NADH dehydrogenase subunit 4 (MT-ND4) gene. Appearance of the former marker indicated sample-derived mtDNA quality, and the tiger marker was present in the tiger samples but absent from non-tiger samples. A blind test of a suspected tiger fecal sample validated reliability of our system, and a parallel experiment using a previously published approach further confirmed the accuracy of our system. This method has significant practical value for the field management and conservation of tigers because it can be used to analyze suspected samples.
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