N. Krithiga, M. Vijayan, V. S. Vasantha, A. Jayachitra
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引用次数: 0
摘要
这项研究描述了壳聚糖/银纳米复合材料的精密沉积过程，并将其作为检测铜绿假单胞菌的免疫分析平台。该薄膜通过紫外（超可见光谱）、傅立叶变换红外（FTIR）、扫描电子显微镜（SEM）、能量色散 X 射线光谱（EDAX）和电化学检测方法进行表征。铜绿假单胞菌的电化学检测是基于银纳米粒子装饰的氧化石墨烯片标记的抗铜绿假单胞菌（Anti Ps）进行的，氧化石墨烯片反过来与抗原结合，并使用过氧化氢（H2O2）在磷酸盐缓冲液（PB）电解液中观察反应。从结果来看，灵敏度范围为 101-107 CFU/毫升，LOD 为 9.36 CFU/毫升。所开发的免疫传感器还具有高稳定性、可重复性和可重复使用性。
An Enzyme Free Electrochemical Assay Utilizing Graphene Oxide Sheets Decorated with Silver Nanoparticles for the Identification of Pseudomonas aeruginosa
This work describes the chronoamperometry deposition of Chitosan/silver nanocomposite and applied as platform for the development of immunoassay for the detection of Pseudomonas aeruginosa. The film was characterized by UV (Ultra visible spectroscopy), FTIR (Fourier transform infra red), Scanning Electron Microscopy (SEM), Energy-Dispersive X-ray spectroscopy (EDAX) and electrochemical detection methods. The electrochemical detection of Pseudomonas aeruginosa was done based on the Anti Ps tagged with silver nanoparticles decorated graphene oxide sheets which in turn binds to the antigen and the response was observed using Hydrogen peroxide (H2O2) in PB (Phosphate Buffer) electrolyte. From the results, the sensitivity range is from 101 to 107 CFU/mL and LOD is calculated as 9.36 CFU/ml. The developed immunosensor also have high stability, reproducibility and reusability.
期刊介绍：
Indian Journal of Microbiology is the official organ of the Association of Microbiologists of India (AMI). It publishes full-length papers, short communication reviews and mini reviews on all aspects of microbiological research, published quarterly (March, June, September and December). Areas of special interest include agricultural, food, environmental, industrial, medical, pharmaceutical, veterinary and molecular microbiology.
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