通过真空沉积技术获得的掺铜羟基磷灰石涂层的物理化学特性

Materials Pub Date : 2024-07-25 DOI:10.3390/ma17153681
Yassine Benali, D. Predoi, Krzysztof Rokosz, C. Ciobanu, S. Iconaru, S. Raaen, C. Negrila, C. Cîmpeanu, R. Trușcǎ, Liliana Ghegoiu, C. Bleotu, I. Marinaș, M. Stan, Khaled Boughzala
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引用次数: 0

摘要

通过真空沉积技术获得了羟基磷灰石和掺铜羟基磷灰石涂层(Ca10-xCux(PO4)6(OH)2;xCu = 0,0.03;HAp 和 3CuHAp)。然后,通过 X 射线衍射 (XRD)、扫描电子显微镜 (SEM)、原子力显微镜 (AFM)、X 射线光电子能谱 (XPS)、傅立叶变换红外光谱 (FTIR) 和水接触角技术对这两种涂层进行了分析。研究还获得了有关体外抗菌活性和生物评价的信息。XRD 研究证实,所获得的薄膜由与羟基磷灰石(HAp)相关的单相组成。获得的二维和三维扫描电镜图像未显示裂缝或其他类型的表面缺陷。傅立叶变换红外光谱研究结果表明,所研究的涂层中存在羟基磷灰石结构特征的振动带。此外,还通过水接触角测量获得了有关 HAp 和 3CuHAp 表面润湿性的信息。使用 HeLa 和 MG63 细胞系评估了 HAp 和 3CuHAp 涂层的生物相容性。涂层的细胞毒性评估是在与 HAp 和 3CuHAp 涂层培养 24、48 和 72 小时后,通过 MTT 法评估细胞活力。使用原子力显微镜研究了铜绿假单胞菌 27853 ATCC(铜绿假单胞菌)细胞附着在 HAp 和 3CuHAp 涂层表面并在其上生长的能力。原子力显微镜研究表明,3CuHAp 涂层抑制了铜绿假单胞菌生物膜的形成。原子力显微镜数据表明,铜绿假单胞菌在 3CuHAp 涂层上的附着和发展在最初的 24 小时内受到了明显的抑制。二维和三维拓扑图都显示,随着时间的推移,附着的细菌细胞迅速减少,暴露 72 小时后观察到明显减少。我们的研究表明,3CuHAp 涂层可作为生物医学用途的合适候选材料,如开发新的抗菌剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Physico-Chemical Properties of Copper-Doped Hydroxyapatite Coatings Obtained by Vacuum Deposition Technique
The hydroxyapatite and copper-doped hydroxyapatite coatings (Ca10−xCux(PO4)6(OH)2; xCu = 0, 0.03; HAp and 3CuHAp) were obtained by the vacuum deposition technique. Then, both coatings were analyzed by the X-ray diffraction (XRD), scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR) and water contact angle techniques. Information regarding the in vitro antibacterial activity and biological evaluation were obtained. The XRD studies confirmed that the obtained thin films consist of a single phase associated with hydroxyapatite (HAp). The obtained 2D and 3D SEM images did not show cracks or other types of surface defects. The FTIR studies’ results proved the presence of vibrational bands characteristic of the hydroxyapatite structure in the studied coating. Moreover, information regarding the HAp and 3CuHAp surface wettability was obtained by water contact angle measurements. The biocompatibility of the HAp and 3CuHAp coatings was evaluated using the HeLa and MG63 cell lines. The cytotoxicity evaluation of the coatings was performed by assessing the cell viability through the MTT assay after incubation with the HAp and 3CuHAp coatings for 24, 48, and 72 h. The results proved that the 3CuHAp coatings exhibited good biocompatible activity for all the tested intervals. The ability of Pseudomonas aeruginosa 27853 ATCC (P. aeruginosa) cells to adhere to and develop on the surface of the HAp and 3CuHAp coatings was investigated using AFM studies. The AFM studies revealed that the 3CuHAp coatings inhibited the formation of P. aeruginosa biofilms. The AFM data indicated that P. aeruginosa’s attachment and development on the 3CuHAp coatings were significantly inhibited within the first 24 h. Both the 2D and 3D topographies showed a rapid decrease in attached bacterial cells over time, with a significant reduction observed after 72 h of exposure. Our studies suggest that 3CuHAp coatings could be suitable candidates for biomedical uses such as the development of new antimicrobial agents.
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