糠醛对湾湾杆菌 ADP1 生理机能的影响。

IF 2.2 4区 生物学 Q3 MICROBIOLOGY
José Eduardo Arteaga, Ernesto Rivera-Becerril, Sylvie Le Borgne, Juan-Carlos Sigala
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引用次数: 0

摘要

木质纤维素生物质的预处理会产生抑制生长的物质,如对微生物有毒的糠醛。刺胞杆菌 ADP1 不能使用糠醛作为碳源,而是在有氧乙酸分解代谢过程中利用依赖于 NADH 的脱氢酶 AreB 和 FrmA 将这种化合物生物转化为二糠醇醚。然而,糠醛生物转化所消耗的 NADH 会影响巴氏杀菌杆菌 ADP1 的有氧生长。根据生长阶段的不同,与乙酸分解和氧化磷酸化有关的几个基因的表达发生了变化,这表明糠醛的存在影响了中心代谢途径。在指数生长阶段,当存在糠醛时,涉及 NADPH(icd 基因)和 NADH(sfcA 基因)形成的反应更受青睐。因此,较高的 NADH 和 NADPH 产量可能会分别支持糠醛的生物转化和生物量的产生。相反,在静止生长阶段,乙醛酸分流基因被过度表达,可能是为了节省碳化合物用于生物质的形成,只有 NADH 的再生受到重视。最后,巴氏杀菌杆菌 ADP1 中 frmA 或 areB 基因的破坏导致生长适应性和糠醛生物转化能力下降。这种生理行为的特征说明了糠醛对醋酸杆菌新陈代谢的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Influence of furfural on the physiology of Acinetobacter baylyi ADP1.

Pretreatment of lignocellulosic biomass produces growth inhibitory substances such as furfural which is toxic to microorganisms. Acinetobacter baylyi ADP1 cannot use furfural as a carbon source, instead it biotransforms this compound into difurfuryl ether using the reduced nicotinamide adenine dinucleotide (NADH)-dependent dehydrogenases AreB and FrmA during aerobic acetate catabolism. However, NADH consumption for furfural biotransformation compromises aerobic growth of A. baylyi ADP1. Depending on the growth phase, several genes related to acetate catabolism and oxidative phosphorylation changed their expression indicating that central metabolic pathways were affected by the presence of furfural. During the exponential growth phase, reactions involved in the formation of reduced nicotinamide adenine dinucleotide phosphate (NADPH) (icd gene) and NADH (sfcA gene) were preferred when furfural was present. Therefore a higher NADH and NADPH production might support furfural biotransformation and biomass production, respectively. In contrast, in the stationary growth phase genes of the glyoxylate shunt were overexpressed probably to save carbon compounds for biomass formation, and only NADH regeneration was appreciated. Finally, disruption of the frmA or areB gene in A. baylyi ADP1 led to a decrease in growth adaptation and in the capacity to biotransform furfural. The characterization of this physiological behavior clarifies the impact of furfural in Acinetobacter metabolism.

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来源期刊
Fems Microbiology Letters
Fems Microbiology Letters 生物-微生物学
CiteScore
4.30
自引率
0.00%
发文量
112
审稿时长
1.9 months
期刊介绍: FEMS Microbiology Letters gives priority to concise papers that merit rapid publication by virtue of their originality, general interest and contribution to new developments in microbiology. All aspects of microbiology, including virology, are covered. 2019 Impact Factor: 1.987, Journal Citation Reports (Source Clarivate, 2020) Ranking: 98/135 (Microbiology) The journal is divided into eight Sections: Physiology and Biochemistry (including genetics, molecular biology and ‘omic’ studies) Food Microbiology (from food production and biotechnology to spoilage and food borne pathogens) Biotechnology and Synthetic Biology Pathogens and Pathogenicity (including medical, veterinary, plant and insect pathogens – particularly those relating to food security – with the exception of viruses) Environmental Microbiology (including ecophysiology, ecogenomics and meta-omic studies) Virology (viruses infecting any organism, including Bacteria and Archaea) Taxonomy and Systematics (for publication of novel taxa, taxonomic reclassifications and reviews of a taxonomic nature) Professional Development (including education, training, CPD, research assessment frameworks, research and publication metrics, best-practice, careers and history of microbiology) If you are unsure which Section is most appropriate for your manuscript, for example in the case of transdisciplinary studies, we recommend that you contact the Editor-In-Chief by email prior to submission. Our scope includes any type of microorganism - all members of the Bacteria and the Archaea and microbial members of the Eukarya (yeasts, filamentous fungi, microbial algae, protozoa, oomycetes, myxomycetes, etc.) as well as all viruses.
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