以往对开普山斑马（Equus zebra zebra）粪便中糖皮质激素代谢物的评估存在缺陷

IF 4.6 1区环境科学与生态学 Q1 ECOLOGY

Functional Ecology Pub Date : 2024-07-25 DOI:10.1111/1365-2435.14621

Jake A. Britnell, Rupert Palme, Graham I. H. Kerley, John Jackson, Susanne Shultz

引用次数: 0

摘要

类固醇激素，尤其是糖皮质激素（GCs）被广泛用于评估对压力源的生理反应。由于类固醇激素在排泄前会进行大量代谢，因此必须对测量粪便糖皮质激素代谢物（fGCMs）的酶免疫测定（EIAs）进行验证。尽管未经验证的检测方法所存在的问题已被多次提出，但其仍被广泛使用。Lea 等人（2017 年）使用未经验证的皮质酮测定法（CJM006）将粪便糖皮质激素浓度与开普山斑马（Equus zebra zebra）的栖息地质量、人口统计和种群表现联系起来。在此，我们将重新审视他们的研究结果，并使用有效的 EIA 评估其结论的有效性。首先，我们通过对开普山斑马和哈特曼山斑马（E. z. hartmannae）这两个山斑马亚种进行生物验证实验（迁移），评估了六种 EIA（三种群体特异性代谢物测定和三种皮质酮测定，包括 CJM006）的生物敏感性。在使用两种 11-oxoaetiocholanolone（实验室代码：72T 和 72a）和一种 11ß-hydroxyaetiocholanolone (69a) EIA 时，移位后 fGCM 的浓度持续上升，而使用三种不同的皮质酮 EIA 时则没有上升。所有皮质酮 EIA（包括 CJM006）都未能检测到转位后 48-72 小时关键时期内 fGCM 的增加。因此，Lea 等人（2017 年）使用的 CJM006 EIA 无法灵敏地测量 CMZ 粪便中的下丘脑-垂体-肾上腺（HPA）轴活性。使用经过验证的检测方法（72T），fGCM 的浓度不再与成年动物性别比或栖息地质量（以草度衡量）相关，因此这些变量已从预测模型中剔除。因此，我们可以得出结论，之前的研究结果并不可靠。我们引入了 "不敏感 "和 "次优 "这两个术语，分别用来描述那些经过测试但未能通过验证的检测方法，以及那些在检测相关激素变化方面相对较差的检测方法。我们将讨论 "不敏感 "和 "次优 "检测方法会如何导致对种群压力因素的错误推断和适得其反的保护建议。在期刊博客上免费阅读本文的通俗摘要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Previous assessments of faecal glucocorticoid metabolites in Cape mountain zebra (Equus zebra zebra) were flawed

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Previous assessments of faecal glucocorticoid metabolites in Cape mountain zebra (Equus zebra zebra) were flawed

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来源期刊

Functional Ecology 环境科学-生态学

CiteScore

9.00

自引率

1.90%

发文量

243

审稿时长

4 months

期刊介绍： Functional Ecology publishes high-impact papers that enable a mechanistic understanding of ecological pattern and process from the organismic to the ecosystem scale. Because of the multifaceted nature of this challenge, papers can be based on a wide range of approaches. Thus, manuscripts may vary from physiological, genetics, life-history, and behavioural perspectives for organismal studies to community and biogeochemical studies when the goal is to understand ecosystem and larger scale ecological phenomena. We believe that the diverse nature of our journal is a strength, not a weakness, and we are open-minded about the variety of data, research approaches and types of studies that we publish. Certain key areas will continue to be emphasized: studies that integrate genomics with ecology, studies that examine how key aspects of physiology (e.g., stress) impact the ecology of animals and plants, or vice versa, and how evolution shapes interactions among function and ecological traits. Ecology has increasingly moved towards the realization that organismal traits and activities are vital for understanding community dynamics and ecosystem processes, particularly in response to the rapid global changes occurring in earth’s environment, and Functional Ecology aims to publish such integrative papers.