间充质干细胞条件培养基调节 HUVEC 对 H2O2 的反应:对基因表达的影响和动脉粥样硬化干预的潜力。

IF 2.6 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BioMed Research International Pub Date : 2024-07-17 eCollection Date: 2024-01-01 DOI:10.1155/2024/7726493
Abdulmajeed Alqasoumi, Mansour Alsharidah, Amer Mahmood, Mona Elsafadi, Osamah Al Rugaie, Khalid M Mohany, Khalid A Al-Regaiey, Khaleel I Alyahya, Alaa A Alanteet, Norah K Algarzae, Hanan K AlGhibiwi, Adel AlHomaidi, Mohammad Abumaree
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引用次数: 0

摘要

背景:我们研究了人骨髓间充质干细胞条件培养基(hBMSC CM)在保护内皮细胞特性(活力、增殖和迁移)免受 H2O2 炎症环境产生的有害影响方面的潜力。此外,我们还研究了它们对内皮细胞某些炎症相关基因(即 TGF-β1、FOS、ATF3、RAF-1 和 SMAD3)表达的影响。方法:在三种条件下单独培养人脐静脉内皮细胞(HUVECs):单独培养、与不同浓度的 H2O2 一起培养或与不同浓度的 H2O2 和 hBMSC CM 一起培养。使用 xCELLigence 系统对 HUVEC 的粘附、增殖和迁移进行了评估。实时聚合酶链反应(RT-PCR)对 HUVEC 的基因表达进行了评估。结果:总体而言,我们观察到在添加了 H2O2 和 hBMSC CM 的培养基中培养的 HUVEC 的活力、增殖和迁移均有所增强。此外,CM 还能调节 HUVEC 中所研究的炎症相关基因的表达,促进更强大的细胞反应。结论本研究揭示了 hBMSC CM 在减轻 H2O2 对内皮细胞功能的破坏作用方面的保护作用。我们的数据表明,即使在氧化应激条件下,hBMSC CM 也能增强 HUVEC 的活力、增殖和迁移。此外,我们还发现条件培养基能调节与炎症有关的关键标志物的基因表达,这表明它对细胞反应机制产生了有利影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Mesenchymal Stem Cell-Conditioned Media Modulate HUVEC Response to H2O2: Impact on Gene Expression and Potential for Atherosclerosis Intervention.

Background: We studied the potential of human bone marrow-derived mesenchymal stem cell conditioned media (hBMSC CM) in protecting endothelial cell properties (viability, proliferation, and migrations) from the deleterious effects produced by the inflammatory environment of H2O2. Additionally, we investigated their impact on the endothelial cells' gene expression of some inflammatory-related genes, namely, TGF-β1, FOS, ATF3, RAF-1, and SMAD3. Methods: Human umbilical vein endothelial cells (HUVECs) were cultured individually under three conditions: alone, with varying concentrations of H2O2, or with varying concentrations of H2O2 and hBMSC CM. HUVEC adhesion, proliferation, and migration were evaluated using the xCELLigence system. The HUVECs' gene expressions were evaluated by real-time polymerase chain reaction (RT-PCR). Results: Generally, we observed enhanced HUVEC viability, proliferation, and migration when cultured in media supplemented with H2O2 and hBMSC CM. Furthermore, the CM modulated the expressions of the studied inflammatory-related genes in HUVECs, promoting a more robust cellular response. Conclusion: This study has illuminated the protective role of hBMSC CM in mitigating the damaging effects of H2O2 on endothelial cell function. Our data demonstrate that hBMSC CM enhances the viability, proliferation, and migration of HUVECs even under oxidative stress conditions. Additionally, the conditioned medium was found to modulate the gene expression of pivotal markers related to inflammation, suggesting a favorable influence on cellular response mechanisms.

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来源期刊
BioMed Research International
BioMed Research International BIOTECHNOLOGY & APPLIED MICROBIOLOGY-MEDICINE, RESEARCH & EXPERIMENTAL
CiteScore
6.70
自引率
0.00%
发文量
1942
审稿时长
19 weeks
期刊介绍: BioMed Research International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies covering a wide range of subjects in life sciences and medicine. The journal is divided into 55 subject areas.
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