使用羟基磷灰石/海藻酸盐复合颗粒进行临界骨缺损再生的生物学评估。

Acta cirurgica brasileira Pub Date : 2024-07-22 eCollection Date: 2024-01-01 DOI:10.1590/acb392824
Iorrana Índira Dos Anjos Ribeiro, Renata Dos Santos Almeida, Ana Maria Guerreiro Braga da Silva, Aryon de Almeida Barbosa Júnior, Alexandre Malta Rossi, Fúlvio Borges Miguel, Fabiana Paim Rosa
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引用次数: 0

摘要

目的:评估羟基磷灰石/海藻酸盐复合材料植入大鼠腓肠肌临界骨缺损后的生物相容性和成骨潜力:结果:GHA组的组织形态学分析表明,在整个研究过程中,类骨质基质(OM)沉积在颗粒之间,并向缺损中心向心方向沉积,120天时有明显的新骨形成,填充了初始骨缺损的4/5。对于 CG,这一发现仅限于骨边缘的边缘,在所有生物点的残留区域都发现了结缔组织的形成。所有生物点的 GHA 炎症反应均为慢性肉芽肿型、离散性和退行性。在整个研究过程中,CG 呈弥漫性和退行性单核炎症浸润。组织形态学分析表明,与 CG 组相比,GHA 组在所有分析期间的 OM 百分比都很明显(P > 0.05)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Biological evaluation of critical bone defect regeneration using hydroxyapatite/ alginate composite granules.

Purpose: to evaluate biocompatibility and osteogenic potential of hydroxyapatite/alginate composite after its implantation on rat calvarian critical bone defect.

Methods: thirty adults male Wistar rats were randomly distributed into two groups: GHA - critical bone defect filled with hydroxyapatite/alginate composite granules (HA/Alg) and CG - critical bone defect without biomaterial; evaluated at biological points of 15, 45 and 120 days.

Results: the histomorphometrically analyses for GHA showed osteoid matrix deposition (OM) among the granules and towards the center of the defect in centripetal direction throughout the study, with evident new bone formation at 120 days, resulting in filling 4/5 of the initial bone defect. For CG, this finding was restricted to the edges of the bone margins and formation of connective tissue on the residual area was found in all biological points. Inflammatory response on GHA was chronic granulomatous type, discrete and regressive for all biological points. Throughout the study, the CG presented mononuclear inflammatory infiltrate diffuse and regressive. Histomorphometry analyses showed that OM percentage was evident for GHA group when compared to CG group in all analyzed periods (p > 0.05).

Conclusions: the biomaterial evaluated at this study showed to be biocompatible, bioactive, osteoconductive and biodegradable synchronously with bone formation.

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