COL5A1的过表达与人类宫颈癌的不良预后有关。

IF 2.3 4区 医学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Xiaoling Duan, Danjuan Ye, Jia Yuan, Bin Guan, Wencai Guan, Songping Liu, Jingyi Fang, Jimin Shi, Yan Zhu, Qinmei Li, Qi Lu, Guoxiong Xu
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引用次数: 0

摘要

背景:宫颈癌是女性最常见的恶性肿瘤:宫颈癌是女性最常见的恶性肿瘤。本研究旨在检测 V 型胶原蛋白 α1 链(COL5A1)的表达及其与宫颈癌患者预后的临床相关性:方法:将宫颈癌组织及其配对的邻近正常组织制备成组织芯片。免疫组化(IHC)染色评估 COL5A1 蛋白的表达及其评分。COL5A1的预后价值由R软件4.2.1版的 "survival, survminer, ggplot2 "软件包和基因表达谱交互式分析(GEPIA)进行分析。cBioPortal 数据库用于分析 COL5A1 基因突变:结果:通过 IHC(P = 0.031)和患者无病生存率(P = 0.042)检测发现,与配对的邻近正常组织相比,COL5A1 蛋白在人类宫颈癌组织中过表达。对癌症基因组图谱-宫颈鳞状细胞癌和GEPIA生存数据集的进一步分析证实,COL5A1高表达与患者总生存率低有关(分别为P = 0.040和P = 0.018)。利用cBioPortal工具对COL5A1基因组改变的分析表明,4%的宫颈癌患者的COL5A1基因发生了改变,COL5A1相应的蛋白质改变的翻译后修饰为羟基化:结论:COL5A1是一种与宫颈癌患者不良预后相关的组织生物标志物,可能会带来新的临床应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
COL5A1 overexpression correlates with poor prognosis in human cervical cancer.

Background: Cervical cancer is the most prevalent malignant tumor in women. This study aims to detect collagen type V α1 chain (COL5A1) expression and its clinical relevance in the prognosis of patients with cervical cancer.

Methods: Cervical cancer tissues and their paired adjacent normal tissues were prepared for tissue microarray. The expression of COL5A1 protein and the scores of the expression were evaluated by immunohistochemistry (IHC) staining. The prognostic value of COL5A1 was analyzed by R software version 4.2.1 with "survival, survminer, ggplot2" packages and Gene Expression Profiling Interactive Analysis (GEPIA). The cBioPortal database was utilized for the analysis of COL5A1 gene mutations.

Results: COL5A1 protein was overexpressed in human cervical cancer tissues compared to their paired adjacent normal tissues detected by IHC (P < 0.001). High expression of COL5A1 tends to be in elderly patients with cervical cancer. Survival analyses of clinical data of patients with cervical cancer showed that a high level of COL5A1 expression was significantly correlated with shorter overall survival (P = 0.031) and disease-free survival (P = 0.042) of patients. Further analyses of The Cancer Genome Atlas-Cervical Squamous Cell Carcinoma and the GEPIA survival datasets confirmed the association of high COL5A1 expression with poor overall survival of patients (P = 0.040 and P = 0.018, respectively). The analysis of genomic alterations of COL5A1 using the cBioPortal tool revealed that the COL5A1 gene was altered in 4% of cervical cancer patients and COL5A1 corresponding protein alterations with post-translational modifications were hydroxylation.

Conclusion: COL5A1 is a tissue biomarker correlated with the poor prognosis of patients with cervical cancer, which may lead to a new clinical application.

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来源期刊
International Journal of Biological Markers
International Journal of Biological Markers 医学-生物工程与应用微生物
CiteScore
4.10
自引率
0.00%
发文量
43
期刊介绍: IJBM is an international, online only, peer-reviewed Journal, which publishes original research and critical reviews primarily focused on cancer biomarkers. IJBM targets advanced topics regarding the application of biomarkers in oncology and is dedicated to solid tumors in adult subjects. The clinical scenarios of interests are screening and early diagnosis of cancer, prognostic assessment, prediction of the response to and monitoring of treatment.
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