新型 FRI 型碳青霉烯酶的出现；位于 IncR 质粒上的 Asburiae 肠杆菌中的 blaFRI-12。

IF 3.7 3区医学 Q2 INFECTIOUS DISEASES

European Journal of Clinical Microbiology & Infectious Diseases Pub Date : 2024-10-01 Epub Date: 2024-07-24 DOI:10.1007/s10096-024-04907-7

Laura F Mataseje, Florence Doualla-Bell, Ken Fakharuddin, Simon Wong, Ariane Yechouron

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引用次数: 0

摘要

肠杆菌因获得流动性碳青霉烯酶而产生的碳青霉烯耐药性令人担忧。一种肠杆菌在 ChromID CARBA 培养基上生长，并在 mCIM 碳青霉烯酶检测试验中呈阳性。药敏试验显示该菌对阿曲南有抗药性，对亚胺培南的敏感性降低。使用 FRI 引物的常规 PCR 检测出了 blaFRI 基因。全基因组测序发现了一种新的变异体；blaFRI-12 与 blaFRI-5 的序列最接近，相差 13 个氨基酸，并存在于一个独特的 110Kb IncR 质粒上。鉴于肠杆菌属本身对碳青霉烯类无敏感性，全球范围内对 blaFRI 类型的报告可能不足。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Emergence of a novel FRI-type carbapenemase; blaFRI-12 in Enterobacter asburiae located on an IncR plasmid.

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Emergence of a novel FRI-type carbapenemase; blaFRI-12 in Enterobacter asburiae located on an IncR plasmid.

Carbapenem-resistance in Enterobacter spp due to acquisition of mobile carbapenemases is of concern. An Enterobacter spp grew on ChromID CARBA medium and was positive for the mCIM carbapenemase detection assay. Susceptibility testing showed resistance to aztreonam and reduced susceptibility to imipenem. Conventional PCR using FRI primers detected a bla_FRI gene. Whole genome sequencing reveled a new variant; bla_FRI-12 was closest in sequence to bla_FRI-5 differing by 13 amino acids and was found on a unique 110Kb IncR plasmid. Given the intrinsic nature of Enterobacter spp. to be carbapenem non-susceptible, blaFRI-types may be under reported globally.

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来源期刊

European Journal of Clinical Microbiology & Infectious Diseases 医学-传染病学

CiteScore

10.40

自引率

2.20%

发文量

138

审稿时长

1 months

期刊介绍： EJCMID is an interdisciplinary journal devoted to the publication of communications on infectious diseases of bacterial, viral and parasitic origin.