Heba A S Bazid, Alaa H Marae, Bassant Farag, Rania Abdallah Abdallah
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引用次数: 0
摘要
背景：斑秃（AA）是一种免疫介导的疾病，以暂时性、非瘢痕性脱发为特征。隆起区受到免疫特权的保护，免受免疫攻击；但最近的研究表明，免疫细胞浸润了隆起区：本研究旨在调查 AA 患者中性决定区 Y-box 9（SOX9）和分化簇 34（CD34）分别作为毛囊干细胞（HFSCs）和祖细胞标志物的免疫组化表达情况：方法：对20名AA患者和20名健康对照者的皮肤样本进行SOX9和CD34免疫组化染色：结果：与皮损周围和对照组皮肤活检样本相比，病例皮损样本中的 SOX9 和 CD34 水平明显较低。此外，在所研究的 AA 患者中，SOX9 水平与脱发严重程度工具评分（SALT 评分）呈负相关。此外，复发患者的SOX9表达也有所降低：结论：在我们研究的AA病例中，干细胞标志物（SOX9和CD34）明显减少，这表明AA患者的高频间充质干细胞及其后代发生了病理变化。研究的局限性：本研究的样本量较小。
The value of immunohistochemical expression of SOX9 and CD34 in alopecia areata.
Background: Alopecia areata (AA), an immune-mediated disorder, is marked by temporary, nonscarring hair loss. The bulge area is protected from immune attacks by immune privilege; however, recent studies demonstrated immune cells infiltrating the bulge area.
Objective: This study aims to investigate the immunohistochemical expression of the sex-determining region Y-box 9 (SOX9) and cluster of differentiation 34 (CD34) in AA patients as markers of hair follicle stem cells (HFSCs) and progenitor cells, respectively.
Methods: Immunohistochemical staining of SOX9 and CD34 was applied on skin samples of 20 AA patients and 20 healthy controls.
Results: SOX9 and CD34 were significantly lower in lesional samples of cases compared to perilesional and control skin biopsies. Furthermore, SOX9 level was negatively correlated with the severity of alopecia tool score (SALT score) among the studied AA patients. Moreover, lowered SOX9 expression was present in patients with recurrent attacks.
Conclusions: The significant reduction of stem cell markers (SOX9 and CD34) in our studied AA cases signifies the pathological affection of HFSCs and their progeny in AA. This is thought to cause a loss of competence in generating new hair in some AA cases, which needs to be validated in further research.
Limitations of the study: This study has a small sample size.
期刊介绍：
The Journal of Immunoassay & Immunochemistry is an international forum for rapid dissemination of research results and methodologies dealing with all aspects of immunoassay and immunochemistry, as well as selected aspects of immunology. They include receptor assay, enzyme-linked immunosorbent assay (ELISA) in all of its embodiments, ligand-based assays, biological markers of ligand-receptor interaction, in vivo and in vitro diagnostic reagents and techniques, diagnosis of AIDS, point-of-care testing, clinical immunology, antibody isolation and purification, and others.
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