肌动蛋白马达蛋白 OsMYA1 与 OsExo70H1 相关联,并通过调节 OsSyp121 的分布促进水稻的分泌防御。

IF 9.3 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Yuan-Bao Li, Chengyu Liu, Ningning Shen, Shuai Zhu, Xianya Deng, Zixuan Liu, Li-Bo Han, Dingzhong Tang
{"title":"肌动蛋白马达蛋白 OsMYA1 与 OsExo70H1 相关联,并通过调节 OsSyp121 的分布促进水稻的分泌防御。","authors":"Yuan-Bao Li,&nbsp;Chengyu Liu,&nbsp;Ningning Shen,&nbsp;Shuai Zhu,&nbsp;Xianya Deng,&nbsp;Zixuan Liu,&nbsp;Li-Bo Han,&nbsp;Dingzhong Tang","doi":"10.1111/jipb.13744","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p><i>Magnaporthe oryzae</i> (<i>M. oryzae</i>) is a devastating hemibiotrophic pathogen. Its biotrophic invasive hyphae (IH) are enclosed in the extrainvasive hyphal membrane produced by plant cells, thus generating a front line of the battlefield between the pathogen and the host plants. In plants, defense-related complexes such as proteins, callose-rich materials and vesicles, are directionally secreted to this interface to confer defense responses, but the underlying molecular mechanism is poorly understood. In this study, we found that a Myosin gene, <i>Myosin A1</i> (<i>OsMYA1</i>), contributed to rice defense. The <i>OsMYA1</i> knockout mutant exhibited decreased resistance to <i>M. oryzae</i> infection. OsMYA1 localizes to the actin cytoskeleton and surrounds the IH of <i>M. oryzae</i>. OsMYA1 interacts with an exocyst subunit, OsExo70H1, and regulates its accumulation at the plasma membrane (PM) and pathogen–plant interface. Furthermore, OsExo70H1 interacted with the rice syntaxin of the plants121 protein (OsSyp121), and the distribution of OsSyp121 to the PM or the pathogen–plant interface was disrupted in both the <i>OsMYA1</i> and <i>OsExo70H1</i> mutants. Overall, these results not only reveal a new function of OsMYA1 in rice blast resistance, but also uncover a molecular mechanism by which plants regulate defense against <i>M. oryzae</i> by OsMYA1-initiated vesicle secretory pathway, which originates from the actin cytoskeleton to the PM.</p></div>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":null,"pages":null},"PeriodicalIF":9.3000,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13744","citationCount":"0","resultStr":"{\"title\":\"The actin motor protein OsMYA1 associates with OsExo70H1 and contributes to rice secretory defense by modulating OsSyp121 distribution\",\"authors\":\"Yuan-Bao Li,&nbsp;Chengyu Liu,&nbsp;Ningning Shen,&nbsp;Shuai Zhu,&nbsp;Xianya Deng,&nbsp;Zixuan Liu,&nbsp;Li-Bo Han,&nbsp;Dingzhong Tang\",\"doi\":\"10.1111/jipb.13744\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n <p><i>Magnaporthe oryzae</i> (<i>M. oryzae</i>) is a devastating hemibiotrophic pathogen. Its biotrophic invasive hyphae (IH) are enclosed in the extrainvasive hyphal membrane produced by plant cells, thus generating a front line of the battlefield between the pathogen and the host plants. In plants, defense-related complexes such as proteins, callose-rich materials and vesicles, are directionally secreted to this interface to confer defense responses, but the underlying molecular mechanism is poorly understood. In this study, we found that a Myosin gene, <i>Myosin A1</i> (<i>OsMYA1</i>), contributed to rice defense. The <i>OsMYA1</i> knockout mutant exhibited decreased resistance to <i>M. oryzae</i> infection. OsMYA1 localizes to the actin cytoskeleton and surrounds the IH of <i>M. oryzae</i>. OsMYA1 interacts with an exocyst subunit, OsExo70H1, and regulates its accumulation at the plasma membrane (PM) and pathogen–plant interface. Furthermore, OsExo70H1 interacted with the rice syntaxin of the plants121 protein (OsSyp121), and the distribution of OsSyp121 to the PM or the pathogen–plant interface was disrupted in both the <i>OsMYA1</i> and <i>OsExo70H1</i> mutants. Overall, these results not only reveal a new function of OsMYA1 in rice blast resistance, but also uncover a molecular mechanism by which plants regulate defense against <i>M. oryzae</i> by OsMYA1-initiated vesicle secretory pathway, which originates from the actin cytoskeleton to the PM.</p></div>\",\"PeriodicalId\":195,\"journal\":{\"name\":\"Journal of Integrative Plant Biology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":9.3000,\"publicationDate\":\"2024-07-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13744\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Integrative Plant Biology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/jipb.13744\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Integrative Plant Biology","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jipb.13744","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

Magnaporthe oryzae(M. oryzae)是一种毁灭性的半生物营养型病原体。它的生物营养侵入性菌丝(IH)被包裹在植物细胞产生的外侵性脑膜中,从而成为病原体与寄主植物之间战场的前线。在植物体内,与防御相关的复合物(如蛋白质、富含胼胝质的物质和囊泡等)会定向分泌到这一界面上,从而产生防御反应,但人们对其潜在的分子机制却知之甚少。在这项研究中,我们发现肌球蛋白基因肌球蛋白 A1(OsMYA1)对水稻的防御有贡献。OsMYA1 基因敲除突变体对 M. oryzae 感染的抵抗力下降。OsMYA1 定位于肌动蛋白细胞骨架,并围绕着口轴霉菌的 IH。OsMYA1 与外囊亚基 OsExo70H1 相互作用,并调节其在质膜(PM)和病原体-植物界面的积累。此外,OsExo70H1 还与水稻植物的句法蛋白 121 蛋白(OsSyp121)相互作用,在 OsMYA1 和 OsExo70H1 突变体中,OsSyp121 在质膜或病原体-植物界面的分布均被破坏。总之,这些结果不仅揭示了OsMYA1在稻瘟病抗性中的新功能,而且揭示了植物通过OsMYA1启动的囊泡分泌途径(从肌动蛋白细胞骨架到PM)调控稻瘟病抗性的分子机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

The actin motor protein OsMYA1 associates with OsExo70H1 and contributes to rice secretory defense by modulating OsSyp121 distribution

The actin motor protein OsMYA1 associates with OsExo70H1 and contributes to rice secretory defense by modulating OsSyp121 distribution

Magnaporthe oryzae (M. oryzae) is a devastating hemibiotrophic pathogen. Its biotrophic invasive hyphae (IH) are enclosed in the extrainvasive hyphal membrane produced by plant cells, thus generating a front line of the battlefield between the pathogen and the host plants. In plants, defense-related complexes such as proteins, callose-rich materials and vesicles, are directionally secreted to this interface to confer defense responses, but the underlying molecular mechanism is poorly understood. In this study, we found that a Myosin gene, Myosin A1 (OsMYA1), contributed to rice defense. The OsMYA1 knockout mutant exhibited decreased resistance to M. oryzae infection. OsMYA1 localizes to the actin cytoskeleton and surrounds the IH of M. oryzae. OsMYA1 interacts with an exocyst subunit, OsExo70H1, and regulates its accumulation at the plasma membrane (PM) and pathogen–plant interface. Furthermore, OsExo70H1 interacted with the rice syntaxin of the plants121 protein (OsSyp121), and the distribution of OsSyp121 to the PM or the pathogen–plant interface was disrupted in both the OsMYA1 and OsExo70H1 mutants. Overall, these results not only reveal a new function of OsMYA1 in rice blast resistance, but also uncover a molecular mechanism by which plants regulate defense against M. oryzae by OsMYA1-initiated vesicle secretory pathway, which originates from the actin cytoskeleton to the PM.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Journal of Integrative Plant Biology
Journal of Integrative Plant Biology 生物-生化与分子生物学
CiteScore
18.00
自引率
5.30%
发文量
220
审稿时长
3 months
期刊介绍: Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信