利用暗视野超光谱显微镜无荧光跟踪蚊子幼虫体内的聚苯乙烯微塑料

IF 1.4 4区化学 Q4 CHEMISTRY, PHYSICAL

Colloid Journal Pub Date : 2024-07-15 DOI:10.1134/s1061933x24600167

M. A. Kryuchkova, I. R. Ishmukhametov, Y. A. Frank, A. V. Simakova, V. V. Yartsev, D. A. Nadueva, A. A. Varenitsina, Y. V. Andreeva, R. F. Fakhrullin

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引用次数: 0

摘要

摘要利用暗视野高光谱显微镜，我们分析了尺寸为 2 μm 的荧光标记聚苯乙烯微球在蚊子幼虫体内的分布，并观察了荧光淬灭和组织学处理引起的光谱特性变化。我们建议将增强型暗视野显微镜与高光谱成像相结合，作为分析聚合物胶体（微塑料）在复杂组织中生物分布的多功能方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Fluorescence-Free Tracking of Polystyrene Microplastics in Mosquito Larvae Using Dark-Field Hyperspectral Microscopy

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Fluorescence-Free Tracking of Polystyrene Microplastics in Mosquito Larvae Using Dark-Field Hyperspectral Microscopy

Abstract

Using dark-field hyperspectral microscopy, we analyzed the distribution of fluorescently-labelled polystyrene microspheres with a size of 2 μm in mosquito larvae and observed the quenching of fluorescence and changes in spectral properties due to histological processing. We propose enhanced dark-field microscopy coupled with hyperspectral imaging as a versatile method for analysing polymer colloids (microplastics) biodistribution in complex tissues.

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来源期刊

Colloid Journal 化学-物理化学

CiteScore

2.20

自引率

18.20%

发文量

审稿时长

6-12 weeks

期刊介绍： Colloid Journal (Kolloidnyi Zhurnal) is the only journal in Russia that publishes the results of research in the area of chemical science dealing with the disperse state of matter and surface phenomena in disperse systems. The journal covers experimental and theoretical works on a great variety of colloid and surface phenomena: the structure and properties of interfaces; adsorption phenomena and structure of adsorption layers of surfactants; capillary phenomena; wetting films; wetting and spreading; and detergency. The formation of colloid systems, their molecular-kinetic and optical properties, surface forces, interaction of colloidal particles, stabilization, and criteria of stability loss of different disperse systems (lyosols and aerosols, suspensions, emulsions, foams, and micellar systems) are also topics of the journal. Colloid Journal also includes the phenomena of electro- and diffusiophoresis, electro- and thermoosmosis, and capillary and reverse osmosis, i.e., phenomena dealing with the existence of diffusion layers of molecules and ions in the vicinity of the interface.