评估作为植物生长促进菌的南极芽孢杆菌菌株

Ángela Zambrano-Solórzano, Ángel Guzmán-Cedeño, M. Pincay, Jonathan Chicaiza, Diego Zambrano
{"title":"评估作为植物生长促进菌的南极芽孢杆菌菌株","authors":"Ángela Zambrano-Solórzano, Ángel Guzmán-Cedeño, M. Pincay, Jonathan Chicaiza, Diego Zambrano","doi":"10.47280/revfacagron(luz).v41.n3.01","DOIUrl":null,"url":null,"abstract":"In agriculture, efficient microorganisms are used, among them plant growth-promoting bacteria. This work aimed to determine, in vitro, the mechanism of action in strains of Bacillus sp. isolated from Antarctica. The analyzed characteristics of the bacterium were: catalase and hemolysis tests, Gram stain, phosphate solubilization, growth without a nitrogen source, siderophore production, and survival at different values of pH, NaCl, and temperature, which confirmed the ecological plasticity and adaptation of these strains in environments other than their origin. According to the desirable characteristics, the T5, GB-70, and B-6 strains were chosen and added to two substrates: clay and clay-compost mixture, which were sterilized and placed in 200 mL glass bottles, and a corn seed was planted in each of them. After two weeks, the following parameters were evaluated: length of root (LR), seedling height (AP), and shoot diameter (DT). The simple effect of the strains as independent variables and their interaction did not significantly affect the response variables evaluated, recording the following averages: 12.84 cm (LR), 15.28 cm (AP), and 2.26 cm (DT). Considering the substrate, the compost + clay significantly (p<0.05) influenced the LR and DT characteristics of the seedlings, with averages of 14.44 and 2.38 cm, respectively. The observed mechanisms of action distinguish promising strains that could be validated at the field level in agricultural production systems when inoculated in organic fertilizers.\nKeywords: antarctic microorganisms, beneficial bacteria, plant growth.","PeriodicalId":509934,"journal":{"name":"Revista de la Facultad de Agronomía, Universidad del Zulia","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Evaluation of Antarctic strains of Bacillus sp. as plant growth promoting bacteria\",\"authors\":\"Ángela Zambrano-Solórzano, Ángel Guzmán-Cedeño, M. Pincay, Jonathan Chicaiza, Diego Zambrano\",\"doi\":\"10.47280/revfacagron(luz).v41.n3.01\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"In agriculture, efficient microorganisms are used, among them plant growth-promoting bacteria. This work aimed to determine, in vitro, the mechanism of action in strains of Bacillus sp. isolated from Antarctica. The analyzed characteristics of the bacterium were: catalase and hemolysis tests, Gram stain, phosphate solubilization, growth without a nitrogen source, siderophore production, and survival at different values of pH, NaCl, and temperature, which confirmed the ecological plasticity and adaptation of these strains in environments other than their origin. According to the desirable characteristics, the T5, GB-70, and B-6 strains were chosen and added to two substrates: clay and clay-compost mixture, which were sterilized and placed in 200 mL glass bottles, and a corn seed was planted in each of them. After two weeks, the following parameters were evaluated: length of root (LR), seedling height (AP), and shoot diameter (DT). The simple effect of the strains as independent variables and their interaction did not significantly affect the response variables evaluated, recording the following averages: 12.84 cm (LR), 15.28 cm (AP), and 2.26 cm (DT). Considering the substrate, the compost + clay significantly (p<0.05) influenced the LR and DT characteristics of the seedlings, with averages of 14.44 and 2.38 cm, respectively. The observed mechanisms of action distinguish promising strains that could be validated at the field level in agricultural production systems when inoculated in organic fertilizers.\\nKeywords: antarctic microorganisms, beneficial bacteria, plant growth.\",\"PeriodicalId\":509934,\"journal\":{\"name\":\"Revista de la Facultad de Agronomía, Universidad del Zulia\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Revista de la Facultad de Agronomía, Universidad del Zulia\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.47280/revfacagron(luz).v41.n3.01\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Revista de la Facultad de Agronomía, Universidad del Zulia","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.47280/revfacagron(luz).v41.n3.01","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

农业中使用了高效微生物,其中包括促进植物生长的细菌。这项工作旨在体外确定从南极洲分离的芽孢杆菌菌株的作用机制。所分析的细菌特征包括:过氧化氢酶和溶血试验、革兰氏染色、磷酸盐溶解、无氮源生长、嗜苷酸产生以及在不同 pH 值、NaCl 值和温度下的存活率,这些特征证实了这些菌株的生态可塑性和对非原产地环境的适应性。根据所需的特性,选择了 T5、GB-70 和 B-6 菌株,并将其添加到两种基质中:粘土和粘土-堆肥混合物,灭菌后置于 200 mL 玻璃瓶中,并在其中各种植一粒玉米种子。两周后,对以下参数进行评估:根长(LR)、苗高(AP)和芽直径(DT)。作为自变量的菌株的简单效应及其交互作用对所评估的响应变量没有显著影响,记录的平均值如下:根长 12.84 厘米(LR)、苗高 12.84 厘米(AP)、芽直径 12.84 厘米(DT):平均值分别为:12.84 厘米(LR)、15.28 厘米(AP)和 2.26 厘米(DT)。就基质而言,堆肥+粘土对秧苗的LR和DT特性有显著影响(p<0.05),平均值分别为14.44厘米和2.38厘米。观察到的作用机制区分了有希望的菌株,这些菌株接种到有机肥料中后可在农业生产系统的田间水平上得到验证。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of Antarctic strains of Bacillus sp. as plant growth promoting bacteria
In agriculture, efficient microorganisms are used, among them plant growth-promoting bacteria. This work aimed to determine, in vitro, the mechanism of action in strains of Bacillus sp. isolated from Antarctica. The analyzed characteristics of the bacterium were: catalase and hemolysis tests, Gram stain, phosphate solubilization, growth without a nitrogen source, siderophore production, and survival at different values of pH, NaCl, and temperature, which confirmed the ecological plasticity and adaptation of these strains in environments other than their origin. According to the desirable characteristics, the T5, GB-70, and B-6 strains were chosen and added to two substrates: clay and clay-compost mixture, which were sterilized and placed in 200 mL glass bottles, and a corn seed was planted in each of them. After two weeks, the following parameters were evaluated: length of root (LR), seedling height (AP), and shoot diameter (DT). The simple effect of the strains as independent variables and their interaction did not significantly affect the response variables evaluated, recording the following averages: 12.84 cm (LR), 15.28 cm (AP), and 2.26 cm (DT). Considering the substrate, the compost + clay significantly (p<0.05) influenced the LR and DT characteristics of the seedlings, with averages of 14.44 and 2.38 cm, respectively. The observed mechanisms of action distinguish promising strains that could be validated at the field level in agricultural production systems when inoculated in organic fertilizers. Keywords: antarctic microorganisms, beneficial bacteria, plant growth.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信