快速筛查中药材中吡虫啉残留的胶体金免疫层析法

IF 2.8 3区 医学 Q2 BIOCHEMICAL RESEARCH METHODS
Yudan Wang , Yanwei Fu , Kun Miao , Mengyue Guo , Xianjun Meng , Jiaoyang Luo , Meihua Yang
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引用次数: 0

摘要

本研究开发了吡虫啉胶体金免疫层析试纸条,并对其系统分析条件进行了深入研究。该试纸可用于中药材中吡虫啉残留量的快速筛查。利用所选的五种中药材(麦芽、薏苡仁、莲子、干姜和金银花)考察了胶体金试纸条的性能。结果表明,所研制的吡虫啉胶体金免疫层析试纸条可用于快速筛查60种不同药材(包括26种根/根茎类药材、20种种子/果实/果皮类药材、11种花/叶/全草类药材和3种树皮/地上部分类药材)中吡虫啉的残留量,临界值为50 μg/kg。该方法的建立可实现现场、快速、低成本地筛查不同药材中吡虫啉的残留量,对保证药材质量具有重要意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A colloidal gold immunochromatographic method for rapid screening of imidacloprid residues in Chinese herbal medicines

An imidacloprid colloidal gold immunochromatographic strip was developed in this work, and systematic analytical conditions were deeply investigated. The test strips were used for rapid screening of imidacloprid residues in Chinese herbal medicines. The performance of the colloidal gold test strips was investigated by using five selected Chinese herbal medicines (malt, Coix seed, lotus seed, dried ginger and honeysuckle). As a result, the developed imidacloprid colloidal gold immunochromatographic test strips could be used for rapid screening of imidacloprid residues in 60 kinds of different herbs (including 26 kinds of root/rhizome medicines, 20 kinds of seed/fruit/pericarp medicines, 11 kinds of flower/leaf/whole herb medicines, and 3 kinds of bark/aboveground issues of herb medicines), and the cut-off value was 50 μg/kg. The development of this method can achieve the goal of on-site, rapid and low-cost screening of imidacloprid residues in different herbs, which is of great significance for the quality assurance of herbs.

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来源期刊
Journal of Chromatography B
Journal of Chromatography B 医学-分析化学
CiteScore
5.60
自引率
3.30%
发文量
306
审稿时长
44 days
期刊介绍: The Journal of Chromatography B publishes papers on developments in separation science relevant to biology and biomedical research including both fundamental advances and applications. Analytical techniques which may be considered include the various facets of chromatography, electrophoresis and related methods, affinity and immunoaffinity-based methodologies, hyphenated and other multi-dimensional techniques, and microanalytical approaches. The journal also considers articles reporting developments in sample preparation, detection techniques including mass spectrometry, and data handling and analysis. Developments related to preparative separations for the isolation and purification of components of biological systems may be published, including chromatographic and electrophoretic methods, affinity separations, field flow fractionation and other preparative approaches. Applications to the analysis of biological systems and samples will be considered when the analytical science contains a significant element of novelty, e.g. a new approach to the separation of a compound, novel combination of analytical techniques, or significantly improved analytical performance.
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