丙戊酸钠自闭症模型大鼠平滑肌组织中的外显子核苷酸酶活性

IF 0.9 4区医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL

Bulletin of Experimental Biology and Medicine Pub Date : 2024-07-02 DOI:10.47056/0365-9615-2024-177-1-57-60

D. V. Ivanova, R. A. Khabirov, A. Ziganshin

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引用次数: 0

摘要

外切核苷酸酶在调节细胞外核苷酸和核苷水平方面发挥着重要作用，也是调节腺苷和 ATP 分别对腺苷和 P2 受体的影响的重要组成部分。我们之前已经证实了 P2 受体激动剂对丙戊酸钠自闭症模型大鼠平滑肌组织收缩活动的模糊影响。在这项工作中，我们使用高效液相色谱法评估了丙戊酸钠自闭症模型大鼠内脏平滑肌组织中外核苷酸酶的活性。十二指肠、输精管和膀胱平滑肌组织中的外切核苷酸酶活性明显较高，而回肠和子宫中的外切核苷酸酶活性较低。根据这些结果，我们可以将此处发现的外切核苷酸酶的活性与之前实验中发现的平滑肌组织中 P2 受体介导的收缩力的变化进行比较。

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Ectonucleotidase Activity in Smooth Muscle Tissues of Rats with a Valproate Model of Autism.

Ectonucleotidases play an important role in regulating the level of extracellular nucleotides and nucleosides and are an important part of the regulation of the effects of adenosine and ATP on adenosine and P2 receptors, respectively. We have previously established the ambiguous effect of P2 receptor agonists on the contractile activity of smooth muscle tissue in rats with the valproate model of autism. In this work, HPLC was used to evaluate the activity of ectonucleotidases in the smooth muscle tissues of the internal organs of rats with a valproate model of autism. The activity of ectonucleotidases was significantly higher in the smooth muscle tissues of the duodenum, vas deferens, and bladder, but lower in the ileum and uterus. The results obtained make it possible to compare the activity of ectonucleotidases identified here with changes in P2 receptor-mediated contractility of smooth muscle tissues revealed in our previous experiments.

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来源期刊

Bulletin of Experimental Biology and Medicine 医学-医学：研究与实验

CiteScore

1.50

自引率

14.30%

发文量

265

审稿时长

2 months

期刊介绍： Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005. World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells. The Russian Volume Year is published in English from April.