{"title":"研究链格孢霉作为一种化学预防剂对人类前列腺癌(du-145)细胞系的生理作用","authors":"Sarder Mohammad Shahriar, Jahan Shayla, Kabir, Sabia Salam, Jahidul Islam, Sarder Mohammad, Shahriar Jahan","doi":"10.22159/ajpcr.2024v17i7.50977","DOIUrl":null,"url":null,"abstract":"Objectives: This study aims to explore the chemopreventive potential of Streblus asper extracts against prostate cancer cells. Specifically, it seeks to assess the efficacy of these extracts in inducing apoptosis and inhibiting cell proliferation within these cancer cells.\nMethods: The inhibitory effects of S. asper extracts were evaluated using the TBEA technique to determine the IC50 values. Morphological changes were indicative of apoptosis, such as cell shrinkage, rounding, vacuolization, formation of apoptotic bodies, membrane blebbing, and cell elongation were observed following treatment. An 8-day cell proliferation assay was conducted to examine the effects on prostate cancer cell line proliferation. In addition, the safety of S. asper root extracts was assessed in vitro using a Mouse fibroblast cell line to ensure no cytotoxic effects were present on normal cells.\nResults: Streblus asper extracts demonstrated a significant inhibitory effect on the proliferation of prostate cancer cells, with no observed cytotoxicity on normal Mouse fibroblast cell lines. The results from the TBEA technique confirmed the extract’s potency, with noticeable morphological changes supporting the induction of apoptosis in the treated cancer cells.\nConclusion: The findings from this study offer promising insights into the potential of S. asper extracts as chemopreventive agents against prostate cancer. By exhibiting significant inhibitory effects on cancer cell proliferation and inducing apoptosis without harming normal cells, S. asper presents a viable, natural alternative in the fight against prostate cancer. Further research and clinical trials are warranted to fully elucidate its therapeutic potential and application in cancer prevention strategies.","PeriodicalId":8528,"journal":{"name":"Asian Journal of Pharmaceutical and Clinical Research","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"STUDY OF THE PHYSIOLOGICAL ROLE OF STREBLUS ASPER AS A CHEMOPREVENTIVE AGENT ON HUMAN PROSTATE CANCER (DU-145) CELL LINE\",\"authors\":\"Sarder Mohammad Shahriar, Jahan Shayla, Kabir, Sabia Salam, Jahidul Islam, Sarder Mohammad, Shahriar Jahan\",\"doi\":\"10.22159/ajpcr.2024v17i7.50977\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objectives: This study aims to explore the chemopreventive potential of Streblus asper extracts against prostate cancer cells. Specifically, it seeks to assess the efficacy of these extracts in inducing apoptosis and inhibiting cell proliferation within these cancer cells.\\nMethods: The inhibitory effects of S. asper extracts were evaluated using the TBEA technique to determine the IC50 values. Morphological changes were indicative of apoptosis, such as cell shrinkage, rounding, vacuolization, formation of apoptotic bodies, membrane blebbing, and cell elongation were observed following treatment. An 8-day cell proliferation assay was conducted to examine the effects on prostate cancer cell line proliferation. In addition, the safety of S. asper root extracts was assessed in vitro using a Mouse fibroblast cell line to ensure no cytotoxic effects were present on normal cells.\\nResults: Streblus asper extracts demonstrated a significant inhibitory effect on the proliferation of prostate cancer cells, with no observed cytotoxicity on normal Mouse fibroblast cell lines. The results from the TBEA technique confirmed the extract’s potency, with noticeable morphological changes supporting the induction of apoptosis in the treated cancer cells.\\nConclusion: The findings from this study offer promising insights into the potential of S. asper extracts as chemopreventive agents against prostate cancer. By exhibiting significant inhibitory effects on cancer cell proliferation and inducing apoptosis without harming normal cells, S. asper presents a viable, natural alternative in the fight against prostate cancer. Further research and clinical trials are warranted to fully elucidate its therapeutic potential and application in cancer prevention strategies.\",\"PeriodicalId\":8528,\"journal\":{\"name\":\"Asian Journal of Pharmaceutical and Clinical Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-07-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Asian Journal of Pharmaceutical and Clinical Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.22159/ajpcr.2024v17i7.50977\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Asian Journal of Pharmaceutical and Clinical Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.22159/ajpcr.2024v17i7.50977","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
研究目的本研究旨在探索天麻提取物对前列腺癌细胞的化学预防潜力。具体而言,研究旨在评估这些提取物在诱导癌细胞凋亡和抑制癌细胞增殖方面的功效:方法:采用 TBEA 技术评估 S. asper 提取物的抑制作用,以确定 IC50 值。处理后观察到了细胞凋亡的形态学变化,如细胞萎缩、变圆、空泡化、凋亡体形成、膜脱落和细胞伸长。还进行了为期 8 天的细胞增殖试验,以研究其对前列腺癌细胞株增殖的影响。此外,还使用小鼠成纤维细胞系对 S. asper 根提取物的安全性进行了体外评估,以确保不会对正常细胞产生细胞毒性作用:结果:白茅根提取物对前列腺癌细胞的增殖有明显的抑制作用,对正常的小鼠成纤维细胞系没有观察到细胞毒性。TBEA技术的结果证实了这种提取物的功效,明显的形态学变化支持了诱导所处理癌细胞凋亡的作用:结论:本研究的结果为了解 S. asper 提取物作为前列腺癌化学预防剂的潜力提供了很好的视角。通过对癌细胞增殖的明显抑制作用以及在不伤害正常细胞的情况下诱导细胞凋亡,S. asper 为抗击前列腺癌提供了一种可行的天然选择。要充分阐明其治疗潜力和在癌症预防策略中的应用,还需要进一步的研究和临床试验。
STUDY OF THE PHYSIOLOGICAL ROLE OF STREBLUS ASPER AS A CHEMOPREVENTIVE AGENT ON HUMAN PROSTATE CANCER (DU-145) CELL LINE
Objectives: This study aims to explore the chemopreventive potential of Streblus asper extracts against prostate cancer cells. Specifically, it seeks to assess the efficacy of these extracts in inducing apoptosis and inhibiting cell proliferation within these cancer cells.
Methods: The inhibitory effects of S. asper extracts were evaluated using the TBEA technique to determine the IC50 values. Morphological changes were indicative of apoptosis, such as cell shrinkage, rounding, vacuolization, formation of apoptotic bodies, membrane blebbing, and cell elongation were observed following treatment. An 8-day cell proliferation assay was conducted to examine the effects on prostate cancer cell line proliferation. In addition, the safety of S. asper root extracts was assessed in vitro using a Mouse fibroblast cell line to ensure no cytotoxic effects were present on normal cells.
Results: Streblus asper extracts demonstrated a significant inhibitory effect on the proliferation of prostate cancer cells, with no observed cytotoxicity on normal Mouse fibroblast cell lines. The results from the TBEA technique confirmed the extract’s potency, with noticeable morphological changes supporting the induction of apoptosis in the treated cancer cells.
Conclusion: The findings from this study offer promising insights into the potential of S. asper extracts as chemopreventive agents against prostate cancer. By exhibiting significant inhibitory effects on cancer cell proliferation and inducing apoptosis without harming normal cells, S. asper presents a viable, natural alternative in the fight against prostate cancer. Further research and clinical trials are warranted to fully elucidate its therapeutic potential and application in cancer prevention strategies.