Dashanga Agada 对多柔比星诱导的小鼠心肌细胞(H9c2)心脏毒性的保护活性

IF 0.1 Q4 MEDICINE, RESEARCH & EXPERIMENTAL
Neha M Saunshimath, Shrutika S Karoshi, Kishore Bhat, Vijay Kumbhar
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引用次数: 0

摘要

背景:癌症仍然是一种复杂而棘手的疾病,会给普通民众带来严重后果。目前,癌症对全球健康构成重大威胁。与癌症相关的风险因素有很多。这些因素包括吸烟、肥胖、过量食用包装食品、缺乏体育锻炼、饮酒等。要改善癌症患者的治疗效果,就必须继续开展预防、研究和早期检测工作。抗癌药物与心脏毒性有关,会损害患者的心脏功能。早期发现心脏毒性对避免不可挽回的心脏损伤至关重要。近年来,心脏肿瘤学已成为一个独特的研究和治疗领域。药物诱发的心脏毒性有几个方面仍然未知。这种毒性在阿育吠陀中被视为 garavisha。可以利用阿加达原则来治疗。根据古代和现代科学,昆虫叮咬可导致心脏症状。Acharya Vagbhata 曾提到 hrudpeeda 是 Vataja Vrischika damsha 的 lakshana 之一。Dashanga Agada(DA)是在 Keeta visha 中提到的配方。由于它是治疗被昆虫叮咬后出现心脏症状的首选配方,因此本研究对它进行了研究,以减少多柔比星(Dox)引起的心脏毒性。研究是在 H9C2 小鼠心肌细胞上进行的。材料与方法:制备阿育吠陀配方 Dashanga agada 的水醇提取物。使用 H9C2 小鼠心肌母细胞研究 Dashanga Agada 试验药物对多柔比星化疗药物诱导的心脏毒性的影响。在 H9C2 细胞株上研究了 MTT 检测、DAPI 核染色、DCFH-DA 检测细胞内 ROS 生成和 SOD 检测这四个参数。研究共分为 4 组。研究了不同浓度和不同时间间隔的 Dashanga agada 对上述参数的影响。研究结果经计算,DA 和 Dox 24 小时的 IC50 值分别为 357.15 和 5.48 µg/ml。与多柔比星处理组相比,Dashanga agada 能有效提高细胞活力、改善细胞核形态和抗氧化活性。结论Dashanga Agada 具有保护心脏的作用。在平行组和预处理组中,平行处理的效果更好。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cardioprotective activity of Dashanga Agada in Doxorubicin induced Cardiotoxicity in Mice Cardiomyoblasts (H9c2)
Background: Cancer is still a complicated and difficult condition with serious consequences for the general population. Currently, it poses a major risk to the global health. There are numerous risk factors associated with cancer. They include tobacco, obesity, excessive consumption of packed foods, lack of physical activity, alcohol consumption, etc. Continued prevention, research & early detection are required to improve outcomes for cancer patients. Anticancer medications have been associated with cardiotoxicity, compromising individuals' heart function. Early detection of cardiotoxicity is essential for avoiding irreparable heart damage. Cardio-oncology has arisen as a distinct area of study and therapeutic practice in recent years. Several aspects of drug-induced cardiotoxicity remain unknown. Such toxicities are considered as garavisha in Ayurveda. They can be treated by using Agada principles. Insect bites can lead to cardiac symptoms, according to both ancient and modern science. Acharya Vagbhata has mentioned hrudpeeda as one of the lakshana of Vataja Vrischika damsha. Dashanga Agada (DA) is the formulation mentioned in context of Keeta visha. As it is the formulation of choice in insect bites exhibiting cardiac symptoms, it is studied in present research to reduce the cardiotoxicity induced by doxorubicin (Dox). The study was carried out on H9C2 mice cardiomyoblasts. Materials and Methods: Hydroalcoholic extract of Ayurvedic formulation Dashanga agada was prepared. H9C2 mice cardiomyoblasts were used to study trial drug Dashanga Agada on cardiotoxicity induced by a chemotherapy drug named Doxorubicin. MTT assay, DAPI nuclear staining, DCFH-DA assay for intracellular ROS generation & SOD assay were the four parameters studied on H9C2 cell line. 4 groups were taken for the study. Dashanga agada was studied at different concentrations & different time intervals on the above mentioned parameters. Results: IC50 values of DA & Dox for 24hrs were calculated to be 357.15 & 5.48 µg/ml respectively. Dashanga agada was effective in increasing cell viability, improving the nuclear morphology & antioxidant activity when compared to Doxorubicin treated group. Conclusion: Dashanga Agada has cardioprotective effect. Within parallel & pre-treatment groups, parallel treatment gave better results.
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来源期刊
International Journal of Ayurvedic Medicine
International Journal of Ayurvedic Medicine MEDICINE, RESEARCH & EXPERIMENTAL-
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