Filipendula glaberrima Nakai 不同生长阶段的植物化学成分、抗氧化、抗原虫和抗炎活性

Pharmaceuticals Pub Date : 2024-07-11 DOI:10.3390/ph17070928
Hak-Dong Lee, Genevieve Tonog, N. Uy, Yunji Lee, Ki-Young Kim, Hangeun Kim, Sanghyun Lee
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引用次数: 0

摘要

由于特应性皮炎是一种炎症性皮肤病,因此具有抗炎特性的天然疗法,如中井扇蕨(Filipendula glaberrima Nakai,FG),可能是很有前景的治疗选择。本研究旨在调查不同生长阶段的菲草提取物的治疗潜力。采用开柱色谱法从 FG 叶提取物中分离出七种化合物,并采用高效液相色谱法对其进行分析。对提取物的总多酚和类黄酮含量(TPC 和 TFC)进行了进一步评估。使用自由基清除试验评估了 FG 提取物的体外抗氧化性,而使用 Griess 试验和 ELISA 分别评估了它们抑制炎症相关生物标志物产生的能力,从而评估了它们的抗炎活性。MTT 试验用于评估全花萃取物在角质细胞系中的活力和细胞毒性。结果表明,全花期表现出最高的 TPC、TFC 和抗氧化活性,从而表明这些特性之间存在正相关。通过抑制脂多糖刺激的巨噬细胞中促炎生物标志物的产生,所有 FG 提取物都显示出明显的抗炎活性。此外,FG 提取物还能抑制角质细胞中细胞因子和趋化因子的产生,这表明它们具有抗异位的潜力。高效液相色谱分析显示,全花期的植物化学物质(没食子酸、(+)-儿茶素、金丝桃素、米曲莲素、黄芪苷、阿夫泽林和槲皮素)含量最高。这些结果表明,由于植物化学成分和生物活性较高,全花期的 FG 是最有希望用于治疗的来源。本研究强调了 FG 提取物(尤其是盛花期)作为天然治疗剂治疗炎症相关疾病的潜力,同时也可为 FG 的进一步研究提供参考。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Phytochemical Profile, Antioxidant, Anti-Atopic, and Anti-Inflammatory Activities of Filipendula glaberrima Nakai at Different Growth Stages
Since atopic dermatitis is an inflammatory skin disease, natural remedies, such as Filipendula glaberrima Nakai (FG), with anti-inflammatory properties are possible promising therapeutic options. This study aimed to investigate the therapeutic potential of FG extracts at different growth stages. Seven compounds were isolated from the FG leaf extracts using open-column chromatography, and they were analyzed using HPLC. The extracts were further evaluated for their total polyphenol and flavonoid content (TPC and TFC). The in vitro antioxidant properties of the FG extracts were evaluated using radical scavenging assays, whereas their anti-inflammatory activities were assessed by evaluating their ability to inhibit the production of inflammation-associated biomarkers using the Griess assay and ELISA, respectively. The MTT assay was used to evaluate the viability and cytotoxicity of the FG extracts in keratinocyte cell lines. The results showed that the full-flowering stage exhibited the highest TPC, TFC, and antioxidant activities, thus suggesting a positive correlation between these properties. All FG extracts showed significant anti-inflammatory activity by inhibiting the production of pro-inflammatory biomarkers in lipopolysaccharide-stimulated macrophages. Additionally, the FG extracts suppressed the production of cytokines and chemokines in keratinocytes, indicating their anti-atopic potential. HPLC analysis revealed that the full-flowering stage had the highest content of all the analyzed phytochemicals (gallic acid, (+)-catechin, hyperin, miquelianin, astragalin, afzelin, and quercetin). These results suggest that the full-flowering stage of FG is the most promising source for therapeutic applications owing to its superior phytochemical profile and biological activities. This study highlights the potential of FG extracts, particularly in its full-flowering stage, as a natural therapeutic agent for the management of inflammation-related diseases, and it can also serve as a reference for further research on FG.
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