五种法医 PCR 检测试剂盒的双联对比分析

DNA Pub Date : 2024-07-11 DOI:10.3390/dna4030014
Tamás Cseppentő, Norbert G. Valis, G. Bárány, Bálint Megadja, Attila Heinrich, Nóra M. Magonyi
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引用次数: 0

摘要

在法医 DNA 实验室中,对市场上销售的短串联重复(STR)位点试剂盒进行内部验证,并研究其单独和组合的有效性非常重要。本研究旨在报告我们实验室在分析低拷贝数(LCN)人类 DNA 样本时使用的法医试剂盒及其组合的比较调查。我们使用了五种部分重叠的多重试剂盒,它们具有不同的标记配置,分别来自不同的生产商:NGM SelectTM PCR扩增试剂盒、NGM DetectTM、GlobalFilerTM扩增试剂盒(Applied BiosystemTM,美国加利福尼亚州福斯特市)、PowerPlex® Fusion 6C系统(Promega Co.,美国威斯康星州麦迪逊)和Investigator® 24plex QS试剂盒(Qiagen GmbH,德国希尔登)。试剂盒的功效通过特定的标准进行检验,如等位基因丢失率、单独计算的共识图谱的似然比(LR)以及由两个试剂盒的组合图谱产生的复合图谱的 LR 值。结果显示,PowerPlex® Fusion 6C 系统和 Investigator® 24plex QS 配对的 LR 值最低,而 NGM DetectTM 和 GlobalFilerTM 试剂盒配对的 LR 值最高。总之,我们的研究有助于为不同质量的样本选择最佳的试剂盒组合。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparative Analysis of Five Forensic PCR Kits in Duplets
In forensic DNA laboratories, it is important to conduct internal validations of the commercially available kits of short tandem repeat (STR) loci and to investigate their individual and combined effectiveness. This study aims to report on a comparative investigation of the forensic kits used in our laboratory and their combinations in analysing low-copy-number (LCN) human DNA samples. We used five partly overlapping multiplex kits with different marker configurations from different manufacturers: the NGM SelectTM PCR Amplification Kit, NGM DetectTM, the GlobalFilerTM Amplification Kit (Applied BiosystemTM, Foster City, CA, USA), the PowerPlex® Fusion 6C System (Promega Co., Madison, WI, USA) and the Investigator® 24plex QS Kit (Qiagen GmbH, Hilden, Germany). The efficacy of the kits was scrutinised by specific criteria, such as allelic dropout rate, the individually calculated Likelihood Ratio (LR) of consensus profiles and the LR value of the composite profile produced by the combined profiles of two kits. According to the results, the pairing of PowerPlex® Fusion 6C System and Investigator® 24plex QS produced the lowest, while the pairing of the NGM DetectTM and GlobalFilerTM kits provided the highest LR value. In summary, our study is meant to aid the selection of the optimal kit combination for samples of different qualities.
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DNA
DNA
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